FastQCFastQC Report
Thu 2 Feb 2017
SRR4064139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120967
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11900.10615834364437132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18100.024.920411
TAGGACG4600.020.5642594
GATATAC3150.020.256641
TCGACGC550.00448230720.0008514
GTATTAG2150.019.4444241
CGGATTT708.120493E-418.85794635
TTAGGAC3350.018.3872013
CGTCGTA3000.018.33329610
TTCGAGT600.007411859518.33329616
AGGACGT5250.018.0182085
ATTACAC1751.9099389E-1017.599183
ACTGTTC3500.017.599188
AACGCAG25900.017.3273776
ATCGTTT2800.017.28490829
TAATAGT1154.274223E-617.2165894
ATATAGG902.2159795E-417.1103133
TACCGTC3600.017.1103137
CCGTCGT3100.017.0322259
GGTATCA8450.016.9253081
GTAGGAC5850.016.9222873
Produced by FastQC (version 0.11.5)