##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064139_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1120967
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34356854394465	32.0	32.0	32.0	32.0	32.0
2	31.403192065422086	32.0	32.0	32.0	32.0	32.0
3	31.478277237420905	32.0	32.0	32.0	32.0	32.0
4	31.564210186383722	32.0	32.0	32.0	32.0	32.0
5	31.510959733872628	32.0	32.0	32.0	32.0	32.0
6	35.11050191486458	36.0	36.0	36.0	36.0	36.0
7	35.11743610650447	36.0	36.0	36.0	36.0	36.0
8	35.064246315904036	36.0	36.0	36.0	36.0	36.0
9	35.176951685464424	36.0	36.0	36.0	36.0	36.0
10	35.033463072507935	36.0	36.0	36.0	36.0	36.0
11	35.177227340323135	36.0	36.0	36.0	36.0	36.0
12	35.08066428360514	36.0	36.0	36.0	36.0	36.0
13	35.12240146230888	36.0	36.0	36.0	36.0	36.0
14	35.05976536329794	36.0	36.0	36.0	36.0	36.0
15	35.037630902604626	36.0	36.0	36.0	36.0	36.0
16	35.04490587144849	36.0	36.0	36.0	36.0	36.0
17	35.01019744559831	36.0	36.0	36.0	36.0	36.0
18	35.00529275170455	36.0	36.0	36.0	36.0	36.0
19	35.001454993768775	36.0	36.0	36.0	36.0	36.0
20	34.99308008175085	36.0	36.0	36.0	36.0	36.0
21	34.99695263107656	36.0	36.0	36.0	36.0	36.0
22	34.963708119864364	36.0	36.0	36.0	36.0	36.0
23	34.92408697133814	36.0	36.0	36.0	32.0	36.0
24	34.89215561207422	36.0	36.0	36.0	32.0	36.0
25	34.860571274622714	36.0	36.0	36.0	32.0	36.0
26	34.80097094740523	36.0	36.0	36.0	32.0	36.0
27	34.792953762242774	36.0	36.0	36.0	32.0	36.0
28	34.765713888098404	36.0	36.0	36.0	32.0	36.0
29	34.74034918066277	36.0	36.0	36.0	32.0	36.0
30	34.72032182927776	36.0	36.0	36.0	32.0	36.0
31	34.71123592398349	36.0	36.0	36.0	32.0	36.0
32	34.66589382203044	36.0	36.0	36.0	32.0	36.0
33	34.65047053124668	36.0	36.0	36.0	32.0	36.0
34	34.645497146659984	36.0	36.0	36.0	32.0	36.0
35	34.61076909489753	36.0	36.0	36.0	32.0	36.0
36	34.57499016474169	36.0	36.0	36.0	32.0	36.0
37	34.566419885687985	36.0	36.0	36.0	32.0	36.0
38	34.53029036537204	36.0	36.0	36.0	32.0	36.0
39	34.52218664777821	36.0	36.0	36.0	32.0	36.0
40	34.5004402448957	36.0	36.0	36.0	32.0	36.0
41	34.489154453253306	36.0	36.0	36.0	32.0	36.0
42	34.41591411700791	36.0	36.0	36.0	32.0	36.0
43	34.415452908069554	36.0	36.0	36.0	32.0	36.0
44	34.33933915985038	36.0	36.0	36.0	32.0	36.0
45	34.316894252908426	36.0	36.0	36.0	32.0	36.0
46	34.289374263470734	36.0	36.0	36.0	32.0	36.0
47	34.265568923973674	36.0	36.0	36.0	32.0	36.0
48	34.2105708731836	36.0	36.0	36.0	32.0	36.0
49	34.19660703660322	36.0	36.0	36.0	32.0	36.0
50	33.60925165504426	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	16.0
21	45.0
22	143.0
23	412.0
24	980.0
25	2193.0
26	4328.0
27	7873.0
28	12797.0
29	19713.0
30	28325.0
31	39840.0
32	57656.0
33	93371.0
34	194790.0
35	658482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7646817413337	17.882974310019495	11.796335865603796	26.556008083043007
2	16.122102701439307	20.47168582587394	36.974266654058404	26.43194481862835
3	18.464586667523076	24.194218894128436	28.67554832367199	28.665646114676502
4	12.457637022320906	16.10002792232064	35.59551708480267	35.846817970555776
5	14.533790914451542	36.76682721257629	33.17020037164341	15.529181501328765
6	34.49206710289795	35.40904488543353	16.59882333525132	13.500064676417194
7	30.219979713943406	30.366817221202762	20.612471196743527	18.8007318681103
8	28.232142427029522	33.0192592645457	19.127770933488677	19.620827374936102
9	27.901976876962603	13.77123180131316	18.024728803882386	40.302062517841854
10	16.136868276416397	26.688974906352676	31.1528233364051	26.021333480825824
11	37.9035243678003	20.59132873670679	22.272020496589107	19.233126398903806
12	25.20939510262122	23.137969271173905	28.60378583847696	23.048849787727917
13	30.10560553238106	19.15467401272476	24.91079106911551	25.82892938577867
14	24.014330522657286	19.424531028650343	24.67643920768196	31.884699241010416
15	25.374114338834204	27.18549326710167	21.824511068645766	25.615881325418364
16	25.944861448867844	25.3148646068809	23.710344187739416	25.029929756511844
17	24.48109534000555	25.569352175398564	24.864335881430943	25.085216603164945
18	25.13938920320225	24.586382053985773	25.966625095230704	24.307603647581274
19	26.379925885352456	24.19065341856934	24.72251611556461	24.70690458051359
20	26.40781116186126	23.92520696545818	24.475092777619185	25.191889095061377
21	27.438274275692326	23.710064613855717	23.85886471234211	24.992796398109846
22	26.517979167930463	23.913524430757814	24.467779518343143	25.100716882968587
23	24.966724831304706	23.714222502934994	25.06904820528192	26.250004460478376
24	25.427177727701743	24.44230937355593	24.82278614261679	25.307726756125543
25	25.467549011911306	24.017531526773674	24.969401009142228	25.545518452172793
26	25.041171758047447	24.969266152443453	25.23458534731478	24.754976742194316
27	25.728131520888702	24.37914671648302	24.51921061059829	25.373511152029987
28	25.049757657237322	24.47505783128876	25.065726497480224	25.409458013993703
29	25.01802039694831	24.37223003304334	24.845310057879374	25.764439512128977
30	24.916364985877948	24.695836180640924	25.12083586840354	25.26696296507759
31	25.758377322771704	24.30233211676553	24.226679501442128	25.71261105902064
32	25.52661159384088	24.507645369065248	24.199867967955466	25.7658750691384
33	25.009032670164355	24.105854865286087	24.961750544859584	25.923361919689974
34	25.706035008024493	24.27322352697684	25.082096056623755	24.938645408374914
35	26.020211535991095	24.1944289812872	24.99776973693601	24.787589745785695
36	25.02774585864065	24.746451028113427	24.623691216249973	25.602111896995954
37	25.929576962192446	24.59132513783075	24.394079968597783	25.08501793137902
38	25.370172942157833	24.40763853884619	24.73309275177425	25.48909576722173
39	25.952778580681073	24.02982518118851	24.38988270474926	25.62751353338115
40	26.321513002364068	24.35175520763638	24.6069851465275	24.719746643472053
41	25.18352851126239	24.535502697282556	25.389248159808304	24.891720631646745
42	26.35286309186088	24.81658512912193	24.551454294370163	24.279097484647032
43	25.365580807810478	24.12244548403327	25.101788116571928	25.410185591584327
44	25.081515169627384	24.716826782829315	24.437511876013083	25.764146171530218
45	25.190908474552074	24.89285930937521	24.55198955177545	25.364242664297258
46	25.070296637536977	24.370987828333472	24.642182978222316	25.916532555907235
47	25.561377011799824	24.262566807232087	24.977050695437526	25.199005485530563
48	26.096447796399087	24.729735275278383	24.015333586692574	25.158483341629957
49	25.27980331250011	25.01476405171968	24.21224194132561	25.493190694454604
50	25.320419321773525	25.338796541871066	24.059617129353327	25.281167007002075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	23.5
2	26.0
3	53.0
4	80.0
5	97.5
6	115.0
7	114.0
8	113.0
9	157.0
10	201.0
11	323.0
12	445.0
13	815.0
14	1185.0
15	1602.0
16	2019.0
17	2172.5
18	2326.0
19	2306.5
20	2287.0
21	2527.0
22	2767.0
23	3229.5
24	3692.0
25	4451.0
26	5210.0
27	6938.5
28	8667.0
29	10373.0
30	12079.0
31	14115.5
32	16152.0
33	19235.5
34	22319.0
35	26475.0
36	30631.0
37	35145.0
38	39659.0
39	42561.5
40	45464.0
41	49604.5
42	53745.0
43	55613.5
44	57482.0
45	63714.0
46	69946.0
47	75462.5
48	80979.0
49	84426.5
50	87874.0
51	86076.0
52	84278.0
53	84011.0
54	83744.0
55	84538.5
56	85333.0
57	82980.0
58	80627.0
59	73781.5
60	66936.0
61	59098.5
62	51261.0
63	44525.0
64	37789.0
65	32114.0
66	26439.0
67	22705.5
68	18972.0
69	17064.0
70	15156.0
71	11906.0
72	8656.0
73	7350.5
74	6045.0
75	4731.0
76	3417.0
77	3029.5
78	2642.0
79	2227.0
80	1812.0
81	1433.0
82	1054.0
83	862.0
84	670.0
85	500.0
86	330.0
87	227.5
88	125.0
89	100.5
90	76.0
91	57.0
92	38.0
93	30.5
94	23.0
95	29.0
96	35.0
97	25.0
98	15.0
99	12.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00909928659808897
2	8.028782292431445E-4
3	4.4604346069063584E-4
4	0.0
5	0.0
6	1.7841738427625434E-4
7	0.0
8	0.0
9	6.244608449668902E-4
10	5.35252152828763E-4
11	0.0
12	0.0
13	2.676260764143815E-4
14	2.676260764143815E-4
15	0.00472806068332074
16	8.028782292431445E-4
17	0.0
18	4.4604346069063584E-4
19	8.920869213812717E-5
20	6.244608449668902E-4
21	0.0
22	2.676260764143815E-4
23	9.81295613519399E-4
24	4.4604346069063584E-4
25	0.0013381303820719076
26	0.00472806068332074
27	0.004460434606906359
28	0.0030330955326963237
29	0.0013381303820719076
30	0.0022302173034531793
31	0.004817269375458867
32	0.0024086346877294335
33	0.003211512916972578
34	0.002141008611315052
35	0.0020517999191769247
36	0.007047486678912047
37	0.0024086346877294335
38	0.006423025833945156
39	0.0031223042248344508
40	0.0015165477663481618
41	0.002141008611315052
42	9.81295613519399E-4
43	9.81295613519399E-4
44	8.920869213812717E-4
45	9.81295613519399E-4
46	2.676260764143815E-4
47	0.001605756458486289
48	0.0025870520720056877
49	8.920869213812717E-5
50	0.0012489216899337803
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1120967.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.44980166262637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41354284802614	49.214325178149124
2	11.3976426046224	13.779704697418484
3	3.131374917725082	5.678729781234125
4	1.336886226203681	3.2325802887803823
5	0.7095565145792441	2.144627528736988
6	0.4398639407741067	1.5953812787001587
7	0.30052466205234435	1.2716659351054422
8	0.20230096497871777	0.9783242567297129
9	0.1634611021763062	0.8893072085500809
>10	0.7799212320162482	8.331560037372896
>50	0.05676185904117837	2.375937052827284
>100	0.06133196765993889	7.5308122575048015
>500	0.005940139256316568	2.397848071943594
>1k	8.910208884474852E-4	0.5791964269469533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1190	0.10615834364437132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.920869213812717E-5
2	0.0	0.0	0.0	0.0	8.920869213812717E-5
3	0.0	0.0	0.0	0.0	8.920869213812717E-5
4	0.0	0.0	0.0	0.0	8.920869213812717E-5
5	0.0	0.0	0.0	0.0	8.920869213812717E-5
6	0.0	0.0	0.0	0.0	8.920869213812717E-5
7	0.0	0.0	0.0	0.0	8.920869213812717E-5
8	0.0	0.0	0.0	8.920869213812717E-5	8.920869213812717E-5
9	0.0	0.0	0.0	1.7841738427625434E-4	8.920869213812717E-5
10	0.0	0.0	0.0	1.7841738427625434E-4	1.7841738427625434E-4
11	0.0	0.0	0.0	1.7841738427625434E-4	1.7841738427625434E-4
12	0.0	0.0	0.0	1.7841738427625434E-4	3.568347685525087E-4
13	0.0	0.0	0.0	1.7841738427625434E-4	5.35252152828763E-4
14	0.0	0.0	0.0	1.7841738427625434E-4	5.35252152828763E-4
15	0.0	0.0	0.0	1.7841738427625434E-4	6.244608449668902E-4
16	0.0	0.0	0.0	1.7841738427625434E-4	8.920869213812717E-4
17	0.0	0.0	0.0	3.568347685525087E-4	8.920869213812717E-4
18	0.0	0.0	0.0	3.568347685525087E-4	9.812956135193988E-4
19	0.0	0.0	0.0	3.568347685525087E-4	9.812956135193988E-4
20	0.0	0.0	0.0	4.4604346069063584E-4	9.812956135193988E-4
21	0.0	0.0	0.0	7.136695371050174E-4	0.001070504305657526
22	0.0	0.0	0.0	8.028782292431446E-4	0.001070504305657526
23	0.0	0.0	0.0	0.0011597129977956532	0.001070504305657526
24	0.0	0.0	0.0	0.0016949651506244163	0.001070504305657526
25	0.0	0.0	0.0	0.0018733825349006707	0.001070504305657526
26	0.0	0.0	0.0	0.0024086346877294335	0.0011597129977956532
27	0.0	0.0	0.0	0.0027654694562819424	0.0011597129977956532
28	0.0	0.0	0.0	0.004817269375458867	0.0011597129977956532
29	0.0	0.0	0.0	0.008117990984569572	0.0011597129977956532
30	0.0	0.0	0.0	0.014630225510652855	0.0011597129977956532
31	0.0	0.0	0.0	0.032204337861863906	0.0012489216899337803
32	0.0	0.0	0.0	0.05084895451873249	0.0012489216899337803
33	0.0	0.0	0.0	0.06824464948566729	0.0012489216899337803
34	0.0	0.0	0.0	0.08804897914033152	0.0013381303820719076
35	0.0	0.0	0.0	0.11133244778838271	0.0013381303820719076
36	0.0	0.0	0.0	0.1450533334165948	0.0014273390742100347
37	0.0	0.0	0.0	0.1881411317193102	0.0014273390742100347
38	0.0	0.0	0.0	0.2528174335194524	0.0014273390742100347
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1810	0.0	24.92041	1
TAGGACG	460	0.0	20.564259	4
GATATAC	315	0.0	20.25664	1
TCGACGC	55	0.004482307	20.00085	14
GTATTAG	215	0.0	19.444424	1
CGGATTT	70	8.120493E-4	18.857946	35
TTAGGAC	335	0.0	18.387201	3
CGTCGTA	300	0.0	18.333296	10
TTCGAGT	60	0.0074118595	18.333296	16
AGGACGT	525	0.0	18.018208	5
ATTACAC	175	1.9099389E-10	17.59918	3
ACTGTTC	350	0.0	17.59918	8
AACGCAG	2590	0.0	17.327377	6
ATCGTTT	280	0.0	17.284908	29
TAATAGT	115	4.274223E-6	17.216589	4
ATATAGG	90	2.2159795E-4	17.110313	3
TACCGTC	360	0.0	17.110313	7
CCGTCGT	310	0.0	17.032225	9
GGTATCA	845	0.0	16.925308	1
GTAGGAC	585	0.0	16.922287	3
>>END_MODULE