FastQCFastQC Report
Thu 2 Feb 2017
SRR4064138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764301
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22900.29962017582078265No Hit
TATCAACGCAGAGTACTTTTTTTTT17710.2317149918683869No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15530.2031921978382862No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9980.13057682771578213No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9920.12979179668743074No Hit
GGTATCAACGCAGAGTACTTTTTTT8680.1135678221015019No Hit
GTACATGGGGTGGTATCAACGCAAA8040.105194157799087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCAC400.005281281714.247795
GGTCTAC602.571815E-514.2459261
TCGAACT759.621199E-713.9375619
CGAGACT551.9628546E-413.8142314
CCGATAA707.2478815E-613.571999
CTAAGCT500.001501562413.2961984
CGAACGA655.436607E-513.15611316
CGGTCCA1301.4551915E-1113.1543910
CGATAAC655.4432938E-513.1543910
TAAGGTT655.4533368E-513.1518075
ACGAACG751.4716548E-512.67050915
ACACGCT751.475625E-512.6671919
TAGGCTG751.4786088E-512.6647025
TTACCCT604.0976677E-412.6630454
GCGCCGA550.003059030312.09457819
TACCGTC1103.799869E-812.091417
TATCCTC550.003069552112.0890355
GCCTTAT550.00307256412.0874521
CTAAATG550.003077086812.0850793
CGTCGTA1209.9717E-911.87549110
Produced by FastQC (version 0.11.5)