##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064138_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 764301 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19416564939729 32.0 32.0 32.0 32.0 32.0 2 30.793260770298613 32.0 32.0 32.0 32.0 32.0 3 30.807464598371585 32.0 32.0 32.0 32.0 32.0 4 30.8292649100289 32.0 32.0 32.0 32.0 32.0 5 30.747742054504705 32.0 32.0 32.0 32.0 32.0 6 34.39652571434553 36.0 36.0 36.0 32.0 36.0 7 34.314124932454625 36.0 36.0 36.0 32.0 36.0 8 34.280471960654246 36.0 36.0 36.0 32.0 36.0 9 34.410856455768084 36.0 36.0 36.0 32.0 36.0 10 34.11037012904602 36.0 36.0 36.0 32.0 36.0 11 34.41162709456091 36.0 36.0 36.0 32.0 36.0 12 34.22782516312291 36.0 36.0 36.0 32.0 36.0 13 34.296686776544846 36.0 36.0 36.0 32.0 36.0 14 34.194479661808636 36.0 36.0 36.0 32.0 36.0 15 34.125865333160625 36.0 36.0 36.0 32.0 36.0 16 34.124190600300146 36.0 36.0 36.0 32.0 36.0 17 34.05682708775731 36.0 36.0 36.0 32.0 36.0 18 34.064139651786405 36.0 36.0 36.0 32.0 36.0 19 34.06545065360375 36.0 36.0 36.0 32.0 36.0 20 34.04750353591059 36.0 36.0 36.0 32.0 36.0 21 34.019115505540356 36.0 36.0 36.0 32.0 36.0 22 34.00005626055703 36.0 36.0 36.0 32.0 36.0 23 33.95589695682722 36.0 36.0 36.0 32.0 36.0 24 33.947094142229304 36.0 36.0 36.0 32.0 36.0 25 33.564127222128455 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 8.0 6 27.0 7 7.0 8 37.0 9 47.0 10 67.0 11 20.0 12 33.0 13 52.0 14 83.0 15 161.0 16 249.0 17 297.0 18 353.0 19 502.0 20 796.0 21 1229.0 22 1888.0 23 2985.0 24 4420.0 25 6355.0 26 9011.0 27 12071.0 28 16495.0 29 21893.0 30 28376.0 31 38261.0 32 53529.0 33 74195.0 34 157391.0 35 333452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.197260177148685 17.459760651630713 11.544376831664248 25.798602339556354 2 16.363343744316374 20.117185711089334 37.59298246532253 25.926488079271763 3 18.824971738402276 24.35469351867359 28.776586836375817 28.043747906548315 4 12.54780661985118 15.81010549839914 36.11151454250226 35.53057333924742 5 14.443723323240546 36.93293485571324 33.666561115458194 14.956780705588022 6 33.30253896512603 35.869899464675235 17.302334832486924 13.525226737711805 7 29.531053291421184 30.83513710874214 21.29052605681173 18.343283543024953 8 27.948228654594192 32.75732541764173 19.90532058027464 19.38912534748944 9 27.55476435130341 14.206857733767041 18.750449961057917 39.487927953871626 10 15.750007527370173 27.105339981855764 32.0295491825276 25.11510330824646 11 36.441610975474056 21.53858237823756 22.782225073145828 19.237581573142553 12 24.55078061074199 23.894609367328904 29.24269302523484 22.31191699669427 13 29.43362039395994 19.939410919126406 25.41821914185543 25.208749545058218 14 23.492445864208843 19.722447697101412 25.962006755518313 30.823099683171428 15 24.924985075564773 27.20790000104733 22.903902347063816 24.963212576324086 16 25.18731973380501 26.026805411357145 24.223194261332466 24.562680593505377 17 23.71144817907368 25.839636992990695 25.62768216463614 24.82123266329948 18 24.19002576833637 25.473205120147487 26.516639431839607 23.820129679676533 19 25.09819992981978 25.128314574964516 26.04249045496682 23.730995040248878 20 25.51721609419356 24.560693671893823 25.514466337743418 24.4076238961692 21 26.45328549824219 24.418810189398155 25.057382469050125 24.070521843309525 22 25.64971795798438 24.424524299608755 25.420313310183367 24.5054444322235 23 24.364067766426338 24.58628067703573 25.80183898533683 25.247812571201102 24 24.428752309410612 25.227209341606837 25.617272230900472 24.72676611808208 25 24.55776024385709 24.838227322364304 25.908244579862412 24.695767853916195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 425.0 1 425.0 2 500.0 3 575.0 4 575.0 5 575.0 6 1307.0 7 2039.0 8 2039.0 9 2039.0 10 2050.5 11 2062.0 12 2062.0 13 2062.0 14 2212.0 15 2362.0 16 2362.0 17 2362.0 18 3933.5 19 5505.0 20 5505.0 21 5505.0 22 8789.0 23 12073.0 24 12073.0 25 12073.0 26 17604.0 27 23135.0 28 23135.0 29 23135.0 30 29009.5 31 34884.0 32 34884.0 33 34884.0 34 42282.0 35 49680.0 36 49680.0 37 49680.0 38 57032.5 39 64385.0 40 64385.0 41 64385.0 42 73304.5 43 82224.0 44 82224.0 45 82224.0 46 90859.5 47 99495.0 48 99495.0 49 99495.0 50 101882.5 51 104270.0 52 104270.0 53 104270.0 54 96208.5 55 88147.0 56 88147.0 57 88147.0 58 80205.5 59 72264.0 60 72264.0 61 72264.0 62 62672.0 63 53080.0 64 53080.0 65 53080.0 66 43168.5 67 33257.0 68 33257.0 69 33257.0 70 25013.0 71 16769.0 72 16769.0 73 16769.0 74 12730.5 75 8692.0 76 8692.0 77 8692.0 78 6891.5 79 5091.0 80 5091.0 81 5091.0 82 3497.0 83 1903.0 84 1903.0 85 1903.0 86 1443.0 87 983.0 88 983.0 89 983.0 90 708.5 91 434.0 92 434.0 93 434.0 94 302.5 95 171.0 96 171.0 97 171.0 98 283.5 99 396.0 100 396.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0405599364648221 2 0.005756894207910234 3 0.0017009005614280236 4 0.0031401241134055824 5 0.007719471778788723 6 0.012037142434721398 7 0.021326676270212914 8 0.03257878767658292 9 0.04527012263493048 10 0.05521384899404816 11 0.054428817965696756 12 0.062279128249210725 13 0.061101581706683616 14 0.06293332077283688 15 0.05926984264053037 16 0.0654192523626163 17 0.06005487366888176 18 0.07497046320755828 19 0.07235369311305363 20 0.07811058732096386 21 0.07405459367448165 22 0.07654052526426107 23 0.08098903442491898 24 0.07562465573118443 25 0.07523214021700875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 764301.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.22048345722916 #Duplication Level Percentage of deduplicated Percentage of total 1 79.57301110545984 49.51071221129176 2 13.013376061769055 16.19397099948007 3 3.7239977038292333 6.951268125775985 4 1.4067329221722038 3.5011041005102093 5 0.6801278389433243 2.115894147588706 6 0.38051537763688337 1.4205510455686203 7 0.2421064367378851 1.0544785679356792 8 0.16603907125287082 0.8264825028914353 9 0.12567752562439805 0.7037744763652517 >10 0.5627630968652202 6.3612593229430985 >50 0.06642319746109893 2.907483328614028 >100 0.05563357561420076 6.5203454916654735 >500 0.0029614831101406197 1.1937733868076768 >1k 6.346035236015614E-4 0.7389022925621056 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2290 0.29962017582078265 No Hit TATCAACGCAGAGTACTTTTTTTTT 1771 0.2317149918683869 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1553 0.2031921978382862 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 998 0.13057682771578213 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 992 0.12979179668743074 No Hit GGTATCAACGCAGAGTACTTTTTTT 868 0.1135678221015019 No Hit GTACATGGGGTGGTATCAACGCAAA 804 0.105194157799087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.308385047252326E-4 7 0.0 0.0 0.0 0.0 1.308385047252326E-4 8 0.0 0.0 0.0 0.0 1.308385047252326E-4 9 0.0 0.0 0.0 0.0 1.308385047252326E-4 10 0.0 0.0 0.0 0.0 1.308385047252326E-4 11 0.0 0.0 0.0 0.0 1.308385047252326E-4 12 0.0 0.0 0.0 0.0 2.616770094504652E-4 13 0.0 0.0 0.0 0.0 2.616770094504652E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACCAC 40 0.0052812817 14.24779 5 GGTCTAC 60 2.571815E-5 14.245926 1 TCGAACT 75 9.621199E-7 13.93756 19 CGAGACT 55 1.9628546E-4 13.814231 4 CCGATAA 70 7.2478815E-6 13.57199 9 CTAAGCT 50 0.0015015624 13.296198 4 CGAACGA 65 5.436607E-5 13.156113 16 CGGTCCA 130 1.4551915E-11 13.15439 10 CGATAAC 65 5.4432938E-5 13.15439 10 TAAGGTT 65 5.4533368E-5 13.151807 5 ACGAACG 75 1.4716548E-5 12.670509 15 ACACGCT 75 1.475625E-5 12.667191 9 TAGGCTG 75 1.4786088E-5 12.664702 5 TTACCCT 60 4.0976677E-4 12.663045 4 GCGCCGA 55 0.0030590303 12.094578 19 TACCGTC 110 3.799869E-8 12.09141 7 TATCCTC 55 0.0030695521 12.089035 5 GCCTTAT 55 0.003072564 12.087452 1 CTAAATG 55 0.0030770868 12.085079 3 CGTCGTA 120 9.9717E-9 11.875491 10 >>END_MODULE