FastQCFastQC Report
Thu 2 Feb 2017
SRR4064138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764301
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.10938098995029447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCG450.001397041524.44655428
CCTATAT450.001397041524.4465541
TAGGACG755.4711396E-520.5324174
ACGGTAT1750.020.1133889
CTATATG550.004483285419.999112
ACGCTTT550.004483285419.9991144
GTAAACG1452.9467628E-1019.7258427
GACGGTA1800.019.5546848
CCCTAGT808.9771136E-519.2516611
ACGACGG1851.8189894E-1219.0261786
CGCTTCG1557.185008E-1018.45320532
CGACGGT1951.8189894E-1218.0504767
ATACACT2700.017.9251274
TACGACG2003.6379788E-1217.5992155
GCACCGA902.2147839E-417.1103486
TCTACAC1802.8558134E-1017.1103483
CGAATGC1802.8558134E-1017.11034843
GTATCAA18850.017.0412581
AAACGCT1702.4592737E-916.82498229
AAGGCGT1053.772859E-516.7611566
Produced by FastQC (version 0.11.5)