##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	764301
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302416194666762	32.0	32.0	32.0	32.0	32.0
2	31.41030824243328	32.0	32.0	32.0	32.0	32.0
3	31.473878746724132	32.0	32.0	32.0	32.0	32.0
4	31.557723985707202	32.0	32.0	32.0	32.0	32.0
5	31.50840310296598	32.0	32.0	32.0	32.0	32.0
6	35.08343571446328	36.0	36.0	36.0	36.0	36.0
7	35.09374186348049	36.0	36.0	36.0	36.0	36.0
8	35.05487236049672	36.0	36.0	36.0	36.0	36.0
9	35.157033681756275	36.0	36.0	36.0	36.0	36.0
10	35.0063325836287	36.0	36.0	36.0	36.0	36.0
11	35.161382753653335	36.0	36.0	36.0	36.0	36.0
12	35.06318714747201	36.0	36.0	36.0	36.0	36.0
13	35.099562868555715	36.0	36.0	36.0	36.0	36.0
14	35.05125598422611	36.0	36.0	36.0	36.0	36.0
15	35.025397062152216	36.0	36.0	36.0	36.0	36.0
16	35.03448117953529	36.0	36.0	36.0	36.0	36.0
17	34.99675258831272	36.0	36.0	36.0	36.0	36.0
18	34.99324219123094	36.0	36.0	36.0	36.0	36.0
19	34.98908152678068	36.0	36.0	36.0	36.0	36.0
20	34.979773675554526	36.0	36.0	36.0	36.0	36.0
21	34.979560408791826	36.0	36.0	36.0	36.0	36.0
22	34.96436875000818	36.0	36.0	36.0	36.0	36.0
23	34.91416470736006	36.0	36.0	36.0	32.0	36.0
24	34.891604223990285	36.0	36.0	36.0	32.0	36.0
25	34.86733368136375	36.0	36.0	36.0	32.0	36.0
26	34.811129384888936	36.0	36.0	36.0	32.0	36.0
27	34.795937726105294	36.0	36.0	36.0	32.0	36.0
28	34.78896534218848	36.0	36.0	36.0	32.0	36.0
29	34.7490543647071	36.0	36.0	36.0	32.0	36.0
30	34.729696807933	36.0	36.0	36.0	32.0	36.0
31	34.72349244603893	36.0	36.0	36.0	32.0	36.0
32	34.68947443481037	36.0	36.0	36.0	32.0	36.0
33	34.6466038903521	36.0	36.0	36.0	32.0	36.0
34	34.630938596181345	36.0	36.0	36.0	32.0	36.0
35	34.59584247567385	36.0	36.0	36.0	32.0	36.0
36	34.568641150541474	36.0	36.0	36.0	32.0	36.0
37	34.56402778486486	36.0	36.0	36.0	32.0	36.0
38	34.49921954831932	36.0	36.0	36.0	32.0	36.0
39	34.5126540459845	36.0	36.0	36.0	32.0	36.0
40	34.481944940540444	36.0	36.0	36.0	32.0	36.0
41	34.454828660436135	36.0	36.0	36.0	32.0	36.0
42	34.40211775203748	36.0	36.0	36.0	32.0	36.0
43	34.377147223410674	36.0	36.0	36.0	32.0	36.0
44	34.31346943154595	36.0	36.0	36.0	32.0	36.0
45	34.27648007787508	36.0	36.0	36.0	32.0	36.0
46	34.24133423873579	36.0	36.0	36.0	32.0	36.0
47	34.20128849759453	36.0	36.0	36.0	32.0	36.0
48	34.15983885929758	36.0	36.0	36.0	32.0	36.0
49	34.11956938431325	36.0	36.0	36.0	32.0	36.0
50	33.54397024209049	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	10.0
21	36.0
22	79.0
23	233.0
24	624.0
25	1475.0
26	3167.0
27	5727.0
28	9199.0
29	14115.0
30	19918.0
31	28094.0
32	39873.0
33	62700.0
34	128799.0
35	450250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4330719376733	18.405953173844033	12.049890674416504	26.111084214066167
2	15.831238821456001	21.02855034482578	36.99677743919592	26.143433394522305
3	17.975117133171356	25.082788063864893	29.160762579140943	27.78133222382281
4	12.284034693137913	16.46170814901459	36.277723043669965	34.97653411417753
5	13.927497150991561	37.51951129201715	33.47974162012087	15.073249936870422
6	33.43630364072652	36.132110773546444	17.08600572028188	13.345579865445153
7	29.401897943349542	31.156573130219638	21.255630962147112	18.185897964283704
8	28.010430445596697	33.156832190459	19.580374747645234	19.252362616299077
9	27.797287431276445	13.943194634525458	18.588265772072006	39.671252162126095
10	15.623278628895616	27.307471388440764	32.09793677522508	24.97131320743855
11	36.69854769069737	21.51380348030878	22.760041868376295	19.027606960617558
12	24.517565723451888	24.02482791465666	29.22578931598938	22.231817045902073
13	29.655487330187086	20.01093815624031	25.38895220051891	24.944622313053692
14	23.69619259453094	19.698809368049194	25.924244406646608	30.680753630773257
15	25.089365621843417	27.2414677420199	22.89814150908834	24.771025127048343
16	25.202048947068867	25.9488809949038	24.29677022615613	24.552299831871203
17	23.70675950966962	25.910603283261434	25.69890658261601	24.68373062445293
18	24.3458384742097	25.318756141248382	26.66731213831236	23.668093246229553
19	25.2575883061778	25.067349120307313	26.13342125680851	23.54164131670638
20	25.56617536422455	24.52796367894596	25.67058531064576	24.235275646183737
21	26.655728567671638	24.3563726856304	25.08605902648302	23.90183972021494
22	25.66495573067608	24.37488469826599	25.652526040201547	24.30763353085638
23	24.427217239416716	24.372526315100846	26.04517889034993	25.15507755513251
24	24.59711553432483	24.983874154292614	25.795203722093785	24.623806589288773
25	24.623442349259182	24.665442003841463	25.969524736618986	24.741590910280365
26	24.440187681381286	25.36838882390347	26.2290749129239	23.96234858179135
27	25.02548213241288	24.941086880005653	25.591519127627866	24.441911859953603
28	24.28955174277187	25.10379171785924	26.18011150495047	24.42654503441842
29	24.397867808301626	25.111607726970604	25.92674974224241	24.56377472248536
30	24.382846488502175	25.424487259167183	25.99836446305322	24.19430178927742
31	24.683020912114685	25.202748853117974	25.437882566608135	24.676347668159202
32	24.56675419900064	25.294881909853473	25.284414665133216	24.853949226012674
33	24.378857403794697	24.91518127589866	25.69238639917203	25.013574921134612
34	24.839882608928892	24.980537314057752	25.829830049863727	24.349750027149632
35	25.301034567129104	24.87435903810248	25.886552197424766	23.938054197343646
36	24.53359746890432	25.45815919838169	25.43041972956635	24.577823603147646
37	25.137811064399934	25.38510554502264	25.3569741623063	24.120109228271126
38	24.471797582706024	25.13755441659263	25.692351778164795	24.698296222536552
39	24.96437828495017	25.20316480456795	25.2787914392743	24.553665471207577
40	25.21785095592738	25.243888397506687	25.496150645571543	24.042110000994395
41	24.335124130224866	25.49321058567694	25.858653526596342	24.31301175750185
42	25.194199591516842	25.65985992246409	25.377899255650004	23.76804123036906
43	24.667275505824968	24.99162628091734	25.688869233909372	24.652228979348315
44	24.561458599101133	25.39451389842927	25.21853472808274	24.825492774386852
45	24.57898889297401	25.530851468877692	25.32896501463448	24.561194623513817
46	24.355842589164105	25.456205931494857	25.16652644993478	25.021425029406263
47	24.47552630545997	25.349016734485602	25.566865980190766	24.608590979863664
48	25.10025579128478	25.729593939512885	24.78336244512917	24.386787824073167
49	24.473537188979705	25.84682172814566	24.98263114304742	24.697009939827215
50	24.573626675563755	25.858994236518623	24.97909838478598	24.588280703131645
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	120.0
2	47.0
3	105.5
4	164.0
5	188.5
6	213.0
7	196.5
8	180.0
9	264.5
10	349.0
11	543.5
12	738.0
13	1319.0
14	1900.0
15	2414.0
16	2928.0
17	3089.0
18	3250.0
19	3207.0
20	3164.0
21	3376.5
22	3589.0
23	3986.5
24	4384.0
25	4829.5
26	5275.0
27	6468.0
28	7661.0
29	8795.0
30	9929.0
31	11424.5
32	12920.0
33	15123.0
34	17326.0
35	19856.5
36	22387.0
37	25352.0
38	28317.0
39	31282.5
40	34248.0
41	37215.5
42	40183.0
43	41938.0
44	43693.0
45	47258.5
46	50824.0
47	53726.5
48	56629.0
49	58292.0
50	59955.0
51	57868.5
52	55782.0
53	55397.0
54	55012.0
55	54292.0
56	53572.0
57	51844.5
58	50117.0
59	45344.0
60	40571.0
61	35414.0
62	30257.0
63	26110.0
64	21963.0
65	18463.0
66	14963.0
67	12666.0
68	10369.0
69	9306.0
70	8243.0
71	6333.0
72	4423.0
73	3841.0
74	3259.0
75	2588.5
76	1918.0
77	1661.5
78	1405.0
79	1144.0
80	883.0
81	712.0
82	541.0
83	418.0
84	295.0
85	216.0
86	137.0
87	95.5
88	54.0
89	40.0
90	26.0
91	21.5
92	17.0
93	17.5
94	18.0
95	17.0
96	16.0
97	13.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009158695330766282
2	2.616770094504652E-4
3	0.0
4	0.0
5	0.0
6	3.925155141756978E-4
7	0.0
8	0.0
9	9.158695330766281E-4
10	0.0015700620567027912
11	1.308385047252326E-4
12	0.0
13	5.233540189009304E-4
14	1.308385047252326E-4
15	0.003270962618130815
16	7.850310283513956E-4
17	0.0
18	2.616770094504652E-4
19	0.0
20	7.850310283513956E-4
21	0.0
22	2.616770094504652E-4
23	7.850310283513956E-4
24	0.0
25	0.0011775465425270934
26	0.00353263962758128
27	0.005233540189009304
28	0.003925155141756978
29	0.0022242545803289543
30	0.0034018011228560473
31	0.0071961177598877935
32	0.0015700620567027912
33	0.0031401241134055824
34	0.0023550930850541867
35	0.002616770094504652
36	0.0061494097220859325
37	0.003925155141756978
38	0.00758863327406349
39	0.002616770094504652
40	0.0022242545803289543
41	0.0030092856086803496
42	0.001308385047252326
43	6.54192523626163E-4
44	7.850310283513956E-4
45	0.001308385047252326
46	0.0010467080378018607
47	0.0014392235519775586
48	7.850310283513956E-4
49	2.616770094504652E-4
50	7.850310283513956E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	764301.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.19871871400925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4110778717377	52.42699247649719
2	12.721475453832321	16.58847799483173
3	3.4517254389735115	6.751442279208721
4	1.3445626961722783	3.50655060084345
5	0.6297209269612123	2.0528498792634613
6	0.3613464498040481	1.4135595323447996
7	0.20811836587509694	0.9498335557136793
8	0.14561749749421427	0.7595259407170576
9	0.11407784076339848	0.6693956146290919
>10	0.5080145699708395	5.930996186402962
>50	0.05758769165198186	2.6703940682573504
>100	0.04465462547948622	5.5210354580516565
>500	0.00202057128391025	0.75894641323889
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	836	0.10938098995029447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.308385047252326E-4
2	0.0	0.0	0.0	0.0	1.308385047252326E-4
3	0.0	0.0	0.0	0.0	1.308385047252326E-4
4	0.0	0.0	0.0	0.0	1.308385047252326E-4
5	0.0	0.0	0.0	0.0	1.308385047252326E-4
6	1.308385047252326E-4	0.0	0.0	0.0	2.616770094504652E-4
7	1.308385047252326E-4	0.0	0.0	0.0	2.616770094504652E-4
8	1.308385047252326E-4	0.0	0.0	0.0	2.616770094504652E-4
9	1.308385047252326E-4	0.0	0.0	0.0	2.616770094504652E-4
10	1.308385047252326E-4	0.0	0.0	0.0	2.616770094504652E-4
11	1.308385047252326E-4	0.0	0.0	0.0	2.616770094504652E-4
12	1.308385047252326E-4	0.0	0.0	0.0	3.925155141756978E-4
13	1.308385047252326E-4	0.0	0.0	0.0	3.925155141756978E-4
14	1.308385047252326E-4	0.0	0.0	0.0	6.54192523626163E-4
15	1.308385047252326E-4	0.0	0.0	0.0	7.850310283513956E-4
16	5.233540189009304E-4	0.0	0.0	0.0	9.158695330766282E-4
17	5.233540189009304E-4	0.0	0.0	0.0	9.158695330766282E-4
18	6.54192523626163E-4	0.0	0.0	0.0	9.158695330766282E-4
19	6.54192523626163E-4	0.0	0.0	0.0	9.158695330766282E-4
20	6.54192523626163E-4	0.0	0.0	0.0	0.001308385047252326
21	6.54192523626163E-4	0.0	0.0	0.0	0.001308385047252326
22	6.54192523626163E-4	0.0	0.0	0.0	0.001308385047252326
23	6.54192523626163E-4	0.0	0.0	2.616770094504652E-4	0.001308385047252326
24	6.54192523626163E-4	0.0	0.0	0.0010467080378018607	0.001308385047252326
25	6.54192523626163E-4	0.0	0.0	0.001308385047252326	0.001308385047252326
26	6.54192523626163E-4	0.0	0.0	0.0018317390661532565	0.0014392235519775586
27	6.54192523626163E-4	0.0	0.0	0.0023550930850541867	0.0014392235519775586
28	9.158695330766282E-4	0.0	0.0	0.004055993646482211	0.0014392235519775586
29	9.158695330766282E-4	0.0	0.0	0.00706527925516256	0.0014392235519775586
30	9.158695330766282E-4	0.0	0.0	0.01556978206230268	0.0014392235519775586
31	9.158695330766282E-4	0.0	0.0	0.030485371600979194	0.0014392235519775586
32	9.158695330766282E-4	0.0	0.0	0.049718631795588386	0.0014392235519775586
33	9.158695330766282E-4	0.0	0.0	0.06816686096184618	0.0014392235519775586
34	9.158695330766282E-4	0.0	0.0	0.08517586657612643	0.0015700620567027912
35	9.158695330766282E-4	0.0	0.0	0.10951182845501968	0.0015700620567027912
36	9.158695330766282E-4	0.0	0.0	0.14418403220720633	0.0015700620567027912
37	9.158695330766282E-4	0.0	0.0	0.19651943409729936	0.0015700620567027912
38	9.158695330766282E-4	0.0	0.0	0.2768542759985922	0.0017009005614280238
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCGCG	45	0.0013970415	24.446554	28
CCTATAT	45	0.0013970415	24.446554	1
TAGGACG	75	5.4711396E-5	20.532417	4
ACGGTAT	175	0.0	20.113388	9
CTATATG	55	0.0044832854	19.99911	2
ACGCTTT	55	0.0044832854	19.99911	44
GTAAACG	145	2.9467628E-10	19.72584	27
GACGGTA	180	0.0	19.554684	8
CCCTAGT	80	8.9771136E-5	19.251661	1
ACGACGG	185	1.8189894E-12	19.026178	6
CGCTTCG	155	7.185008E-10	18.453205	32
CGACGGT	195	1.8189894E-12	18.050476	7
ATACACT	270	0.0	17.925127	4
TACGACG	200	3.6379788E-12	17.599215	5
GCACCGA	90	2.2147839E-4	17.110348	6
TCTACAC	180	2.8558134E-10	17.110348	3
CGAATGC	180	2.8558134E-10	17.110348	43
GTATCAA	1885	0.0	17.041258	1
AAACGCT	170	2.4592737E-9	16.824982	29
AAGGCGT	105	3.772859E-5	16.761156	6
>>END_MODULE