FastQCFastQC Report
Thu 2 Feb 2017
SRR4064136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962568
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT37640.39103730853300755No Hit
CTGTAGGACGTGGAATATGGCAAGA32290.3354568196740386No Hit
GTCCTACAGTGGACATTTCTAAATT31910.3315090466335885No Hit
GTATCAACGCAGAGTACTTTTTTTT29240.3037707465862152No Hit
CTTTAGGACGTGAAATATGGCGAGG28160.29255076005019903No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23160.24060637793901313No Hit
TATCAACGCAGAGTACTTTTTTTTT21930.2278280599396614No Hit
GATATACACTGTTCTACAAATCCCG20360.21151752395674903No Hit
TTGTAGAACAGTGTATATCAATGAG20320.21110196889985955No Hit
GTCCTACAGTGTGCATTTCTCATTT20130.2091280823796345No Hit
CTGTAGGACCTGGAATATGGCGAGA16390.17027368456046743No Hit
CTGAAGGACCTGGAATATGGCGAGA15860.1647675800566817No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15800.16414424747134748No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15150.1573914777968933No Hit
ATTTAGAAATGTCCACTGTAGGACG15100.15687203397578145No Hit
GTGTATATCAATGAGTTACAATGAA14970.15552148004089061No Hit
GTATCTGATCGTCTTCGAACCTCCG14740.15313203846377607No Hit
GAATAGGACCGCGGTTCTATTTTGT14690.15261259464266422No Hit
GAATAACGCCGCCGCATCGCCAGTC14600.15167759576466286No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14430.14991148677288255No Hit
GTCCTTCAGTGTGCATTTCTCATTT14320.14876871036643646No Hit
GAACTACGACGGTATCTGATCGTCT14210.14762593395999035No Hit
GCGCAAGACGGACCAGAGCGAAAGC13980.1452364923828758No Hit
GATTAAGAGGGACGGCCGGGGGCAT13980.1452364923828758No Hit
GTACATGGGGTGGTATCAACGCAAA13720.14253538451309414No Hit
CTATTGGAGCTGGAATTACCGCGGC13480.14004205417175722No Hit
GTGTATATCAATGAGTTACAATGAG13170.13682150248086367No Hit
GTCCTATTCCATTATTCCTAGCTGC13030.13536705978175048No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12480.12965317774952No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG11660.12113429908328555No Hit
GATATACACTGTTCTACAATGCCGG11560.12009541144106183No Hit
ATCAGATACCGTCGTAGTTCCGACC11460.11905652379883812No Hit
CTTTAATATACGCTATTGGAGCTGG11440.11884874627039335No Hit
TTTCTAAATTTTCCACCTTTTTCAG11140.11573208334372222No Hit
CTCTTAATCATGGCCTCAGTTCCGA11080.11510875075838797No Hit
GTTCTACAGTGTGGTTTTTATCATT11050.11479708446572087No Hit
GTCCTGTATTGTTATTTTTCGTCAC11020.11448541817305376No Hit
GCTTTGAACACTCTAATTTTTTCAA10610.11022597883993651No Hit
GAATAATGGAATAGGACCGCGGTTC10600.11012209007571414No Hit
GGGTAGGCACACGCTGAGCCAGTCA10250.10648598332793112No Hit
GAATATGGCAAGAAAACTGAAAATC10050.10440820804348368No Hit
ATACAGGACTCTTTCGAGGCCCTGT9930.10316154287281522No Hit
GGTATCAACGCAGAGTACTTTTTTT9790.101707100173702No Hit
GTGCATGGCCGTTCTTAGTTGGTGG9730.10108376758836779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC250.006050584418.9890353
CCCAATT653.3654524E-614.61682413
GTTCTAA851.7163984E-814.5278211
CCAACGT1058.731149E-1114.47987619
GCGTTAT400.005280999514.2484411
TCCAACG2900.014.08979218
CCAACGA1900.014.00356419
AAATCGC759.645282E-713.93543114
AATCGCT759.645282E-713.93543115
CGGTCCA2750.013.82098110
AGAATTT3000.013.61871617
TCCCCGC1051.369699E-913.5699431
ATAGGGT852.714405E-713.4040243
ACGGTAT2350.013.3431289
GTCTTCG2500.013.30200311
AATCGTA500.001497709513.301311513
GTCTTAG1001.0068106E-813.2985451
TCGCCAG2450.013.18565817
CGCCAGT2600.013.15719518
CGCCCCT1301.4551915E-1113.1496725
Produced by FastQC (version 0.11.5)