##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064136_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	962568
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278883154229103	32.0	32.0	32.0	32.0	32.0
2	30.997573158467766	32.0	32.0	32.0	32.0	32.0
3	31.017073079512304	32.0	32.0	32.0	32.0	32.0
4	31.0387099924369	32.0	32.0	32.0	32.0	32.0
5	30.986603543853526	32.0	32.0	32.0	32.0	32.0
6	34.63121774253871	36.0	36.0	36.0	32.0	36.0
7	34.56435077833462	36.0	36.0	36.0	32.0	36.0
8	34.52316199998338	36.0	36.0	36.0	32.0	36.0
9	34.60298804863656	36.0	36.0	36.0	32.0	36.0
10	34.38424298335286	36.0	36.0	36.0	32.0	36.0
11	34.583023744815954	36.0	36.0	36.0	32.0	36.0
12	34.42218316004688	36.0	36.0	36.0	32.0	36.0
13	34.50155729257569	36.0	36.0	36.0	32.0	36.0
14	34.42742746486482	36.0	36.0	36.0	32.0	36.0
15	34.37313623556985	36.0	36.0	36.0	32.0	36.0
16	34.375476849427784	36.0	36.0	36.0	32.0	36.0
17	34.29547003432485	36.0	36.0	36.0	32.0	36.0
18	34.304200846070096	36.0	36.0	36.0	32.0	36.0
19	34.28299922706759	36.0	36.0	36.0	32.0	36.0
20	34.24448038995686	36.0	36.0	36.0	32.0	36.0
21	34.20745235661273	36.0	36.0	36.0	32.0	36.0
22	34.176047822075944	36.0	36.0	36.0	32.0	36.0
23	34.16337339284082	36.0	36.0	36.0	32.0	36.0
24	34.12100547701565	36.0	36.0	36.0	32.0	36.0
25	33.751723514598446	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	9.0
5	13.0
6	41.0
7	8.0
8	50.0
9	63.0
10	91.0
11	32.0
12	68.0
13	51.0
14	100.0
15	166.0
16	244.0
17	359.0
18	435.0
19	577.0
20	860.0
21	1241.0
22	1792.0
23	2729.0
24	4163.0
25	6055.0
26	8756.0
27	11703.0
28	16883.0
29	22992.0
30	31919.0
31	44176.0
32	63840.0
33	92720.0
34	204335.0
35	446094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.90421631149653	18.079483000889656	11.343029491731174	25.67327119588263
2	14.80299008327143	20.338799914806522	41.7468818667761	23.111328135145946
3	19.194059963451362	25.243700866341907	29.338020867422365	26.224218302784365
4	11.22214903577432	15.814714077632583	38.171223042510704	34.79191384408239
5	12.55558557060926	38.78844235724379	34.80311279195411	13.852859280192837
6	32.001799575873626	37.78932701888619	17.84323117380765	12.365642231432522
7	28.37047828454335	31.218800527039893	21.638354205720127	18.77236698269663
8	25.894536649241868	36.76179291853299	19.894723714704384	17.44894671752076
9	27.48302105659232	13.65175651768991	19.369923727280856	39.49529869843692
10	15.532201310817623	27.333510049428533	33.020961647409806	24.113326992344035
11	37.10030342688389	20.155382838657772	24.934642119476806	17.809671614981536
12	25.42965436369987	23.48825701501971	31.29750835814441	19.784580263136007
13	30.203260991604257	20.077740675901016	26.131803608631106	23.58719472386362
14	21.759502316776118	22.55480576402671	25.96598180470466	29.71971011449251
15	23.05949055617178	32.19706617073011	22.623917434463916	22.119525838634193
16	22.87654471151697	26.007291734890238	28.73737799027145	22.378785563321347
17	21.38741243038872	27.6506342851856	28.023938899047664	22.93801438537802
18	22.33446803732254	25.139169330136475	31.584523663464957	20.941838969076024
19	25.413176740665573	24.414025135784804	26.16165669941028	24.01114142413934
20	24.932780186531083	25.92137413310875	26.850598375911083	22.295247304449088
21	25.839536721284205	24.559178241703403	24.706292106795306	24.894992930217082
22	24.717896678391295	27.44655034919156	25.824163827351626	22.011389145065518
23	22.11085058929342	26.666735985110396	27.495334002256318	23.727079423339866
24	24.466682608831267	25.938906852704797	27.555962196275775	22.038448342188165
25	23.31399779784921	26.418219418644668	27.768206473990602	22.49957630951552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129.0
1	129.0
2	344.5
3	560.0
4	560.0
5	560.0
6	1820.5
7	3081.0
8	3081.0
9	3081.0
10	2958.0
11	2835.0
12	2835.0
13	2835.0
14	2742.0
15	2649.0
16	2649.0
17	2649.0
18	4832.0
19	7015.0
20	7015.0
21	7015.0
22	13179.0
23	19343.0
24	19343.0
25	19343.0
26	32772.0
27	46201.0
28	46201.0
29	46201.0
30	62086.0
31	77971.0
32	77971.0
33	77971.0
34	83648.5
35	89326.0
36	89326.0
37	89326.0
38	90957.5
39	92589.0
40	92589.0
41	92589.0
42	98811.5
43	105034.0
44	105034.0
45	105034.0
46	114489.0
47	123944.0
48	123944.0
49	123944.0
50	118318.0
51	112692.0
52	112692.0
53	112692.0
54	95951.0
55	79210.0
56	79210.0
57	79210.0
58	72797.5
59	66385.0
60	66385.0
61	66385.0
62	59460.0
63	52535.0
64	52535.0
65	52535.0
66	44431.5
67	36328.0
68	36328.0
69	36328.0
70	27263.5
71	18199.0
72	18199.0
73	18199.0
74	14688.5
75	11178.0
76	11178.0
77	11178.0
78	9772.5
79	8367.0
80	8367.0
81	8367.0
82	5783.5
83	3200.0
84	3200.0
85	3200.0
86	2596.5
87	1993.0
88	1993.0
89	1993.0
90	1443.5
91	894.0
92	894.0
93	894.0
94	598.0
95	302.0
96	302.0
97	302.0
98	455.0
99	608.0
100	608.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04155550568894873
2	0.005506104503785706
3	0.0015583314633355772
4	0.003532217983560642
5	0.00914221125156872
6	0.012362762942462246
7	0.022024418015142825
8	0.03511440230716168
9	0.051321049525851675
10	0.05952826189941906
11	0.05786604167186111
12	0.06565769898853899
13	0.06773547427298643
14	0.0697093607932115
15	0.06409936752520341
16	0.06825491809409828
17	0.06430714505364815
18	0.08207212373567374
19	0.07812435069522361
20	0.08290323384945272
21	0.07563102035388669
22	0.08051379227233815
23	0.08550045295501202
24	0.07874768328055784
25	0.0811371248576724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	962568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.51800422303836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.62292401238416	32.22748886950752
2	13.387870984748076	11.116753681645267
3	4.048551807994313	5.042633731844924
4	1.6071269671614412	2.6689881683827017
5	0.7966245541252834	1.6537130801174784
6	0.44982284708135095	1.12054481188456
7	0.28941893765555393	0.8411267673067393
8	0.19552338061343036	0.6494192433608911
9	0.15129460709040532	0.5653305122492138
>10	1.0537280671603386	8.914274843158749
>50	0.17158960781522387	5.088698404507647
>100	0.1914071018563226	16.522034162940066
>500	0.023831814778274257	6.59869003556598
>1k	0.010285309535886786	6.9903036875283515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3764	0.39103730853300755	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3229	0.3354568196740386	No Hit
GTCCTACAGTGGACATTTCTAAATT	3191	0.3315090466335885	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2924	0.3037707465862152	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2816	0.29255076005019903	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2316	0.24060637793901313	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2193	0.2278280599396614	No Hit
GATATACACTGTTCTACAAATCCCG	2036	0.21151752395674903	No Hit
TTGTAGAACAGTGTATATCAATGAG	2032	0.21110196889985955	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2013	0.2091280823796345	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1639	0.17027368456046743	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1586	0.1647675800566817	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1580	0.16414424747134748	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1515	0.1573914777968933	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1510	0.15687203397578145	No Hit
GTGTATATCAATGAGTTACAATGAA	1497	0.15552148004089061	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1474	0.15313203846377607	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1469	0.15261259464266422	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1460	0.15167759576466286	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1443	0.14991148677288255	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1432	0.14876871036643646	No Hit
GAACTACGACGGTATCTGATCGTCT	1421	0.14762593395999035	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1398	0.1452364923828758	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1398	0.1452364923828758	No Hit
GTACATGGGGTGGTATCAACGCAAA	1372	0.14253538451309414	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1348	0.14004205417175722	No Hit
GTGTATATCAATGAGTTACAATGAG	1317	0.13682150248086367	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1303	0.13536705978175048	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1248	0.12965317774952	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1166	0.12113429908328555	No Hit
GATATACACTGTTCTACAATGCCGG	1156	0.12009541144106183	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1146	0.11905652379883812	No Hit
CTTTAATATACGCTATTGGAGCTGG	1144	0.11884874627039335	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1114	0.11573208334372222	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1108	0.11510875075838797	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1105	0.11479708446572087	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1102	0.11448541817305376	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1061	0.11022597883993651	No Hit
GAATAATGGAATAGGACCGCGGTTC	1060	0.11012209007571414	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1025	0.10648598332793112	No Hit
GAATATGGCAAGAAAACTGAAAATC	1005	0.10440820804348368	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	993	0.10316154287281522	No Hit
GGTATCAACGCAGAGTACTTTTTTT	979	0.101707100173702	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	973	0.10108376758836779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.0777752844474364E-4
6	0.0	0.0	0.0	0.0	2.0777752844474364E-4
7	0.0	0.0	0.0	0.0	2.0777752844474364E-4
8	0.0	0.0	0.0	0.0	2.0777752844474364E-4
9	0.0	0.0	0.0	1.0388876422237182E-4	2.0777752844474364E-4
10	0.0	0.0	0.0	2.0777752844474364E-4	2.0777752844474364E-4
11	0.0	0.0	0.0	3.1166629266711547E-4	2.0777752844474364E-4
12	0.0	0.0	0.0	3.1166629266711547E-4	2.0777752844474364E-4
13	0.0	0.0	0.0	3.1166629266711547E-4	2.0777752844474364E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGAC	25	0.0060505844	18.989035	3
CCCAATT	65	3.3654524E-6	14.616824	13
GTTCTAA	85	1.7163984E-8	14.527821	1
CCAACGT	105	8.731149E-11	14.479876	19
GCGTTAT	40	0.0052809995	14.248441	1
TCCAACG	290	0.0	14.089792	18
CCAACGA	190	0.0	14.003564	19
AAATCGC	75	9.645282E-7	13.935431	14
AATCGCT	75	9.645282E-7	13.935431	15
CGGTCCA	275	0.0	13.820981	10
AGAATTT	300	0.0	13.618716	17
TCCCCGC	105	1.369699E-9	13.569943	1
ATAGGGT	85	2.714405E-7	13.404024	3
ACGGTAT	235	0.0	13.343128	9
GTCTTCG	250	0.0	13.302003	11
AATCGTA	50	0.0014977095	13.3013115	13
GTCTTAG	100	1.0068106E-8	13.298545	1
TCGCCAG	245	0.0	13.185658	17
CGCCAGT	260	0.0	13.157195	18
CGCCCCT	130	1.4551915E-11	13.149672	5
>>END_MODULE