FastQCFastQC Report
Thu 2 Feb 2017
SRR4064135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005596
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28380.282220693001961No Hit
TATCAACGCAGAGTACTTTTTTTTT17280.17183839235637374No Hit
GTCCTAAAGTGTGTATTTCTCATTT11390.11326616255434586No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10740.10680233413816284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT907.2759576E-1216.8773042
GTTATTC1200.015.8279833
GCGTTAT1004.1836756E-1115.1918411
GCGTGTA456.756298E-414.7786689
TAACACA653.3723736E-614.6140824
CGAACGA759.640098E-713.936254516
CTCTTAC551.9680097E-413.8107641
ATCGCCA903.6236088E-813.72509916
CGCCAGT852.6873022E-713.41524418
CGCGGGA500.001498222913.30080211
CGCATCG905.3913936E-712.6680613
TTGCGCG1457.2759576E-1212.45150618
ACGAACG853.930172E-612.29669515
GCCTTAT701.0956379E-412.2077291
CGACCCG1103.812056E-812.0898325
TCCCGAC1103.8438884E-812.0826162
TGGCGAG5950.011.81819118
TAGAAAT1701.8189894E-1211.7342494
GTATCAA17750.011.7148351
TTTGCGC1552.5465852E-1111.64760217
Produced by FastQC (version 0.11.5)