FastQCFastQC Report
Thu 2 Feb 2017
SRR4064135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005596
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA300.00574430529.333622
TGCAACG450.00139919224.441025
GTATCAA22450.022.8343031
TGTAGGA8800.020.7481252
TAGGACG8900.020.0196674
CTATAAG1452.9467628E-1019.7252961
GGCGTTA2250.019.5557342
TCCTACA8400.019.37922
GTAGGAC9450.019.3200443
TATAAGG1605.2750693E-1119.2482592
GGACGTG8850.019.1385616
TAGGACC6700.019.0421094
GTCTAGG1404.0763553E-918.8582521
CTATATT708.1296806E-418.8545024
TAGACAG1759.094947E-1218.85455
AGGACGT9350.018.585635
CTGTAGG9850.018.5391621
CTAGATA1901.8189894E-1218.523723
GTCCTAC7550.018.3586941
CCTACAG8200.018.2413463
Produced by FastQC (version 0.11.5)