FastQCFastQC Report
Thu 2 Feb 2017
SRR4064134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633813
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21930.34600110758220487No Hit
TATCAACGCAGAGTACTTTTTTTTT16430.2592247240116564No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15340.2420272225404023No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9180.14483767294138808No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8140.12842904768441166No Hit
GTACATGGGGTGGTATCAACGCAAA7680.12117138651305669No Hit
GGTATCAACGCAGAGTACTTTTTTT7640.12054028554163451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGTC250.006036763518.995343
ATTATAC350.002171844716.2817213
GTTTATA456.771127E-414.7729881
GCGTTAT456.771127E-414.7729881
TGCGAAT400.005269294614.25213319
AACCGCG602.5637215E-514.2498817
ACTTTGC602.5637215E-514.2498818
CTAATGT400.00527758614.2487564
TTTAAAC400.0052831214.2465063
CCAAGAC602.569457E-514.2465063
CGTTATT400.005294202414.2420082
CTAGAAC551.9613419E-413.8147943
TAAGACT903.6294296E-813.72102454
ACCGTCG1351.8189894E-1213.3702588
AATTAAC500.001504183513.2925412
CTTAATC655.4550794E-513.15061953
GTGCTAC655.4591233E-513.1495821
ATACCGT1450.013.1033386
TCGCACG751.4730396E-512.66856318
CGCACGC751.4730396E-512.66856319
Produced by FastQC (version 0.11.5)