##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064134_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633813
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18922300426151	32.0	32.0	32.0	32.0	32.0
2	30.74731190429985	32.0	32.0	32.0	32.0	32.0
3	30.768925534818628	32.0	32.0	32.0	32.0	32.0
4	30.78450110679333	32.0	32.0	32.0	32.0	32.0
5	30.64196695239763	32.0	32.0	32.0	32.0	32.0
6	34.314930429006665	36.0	36.0	36.0	32.0	36.0
7	34.24880209146862	36.0	36.0	36.0	32.0	36.0
8	34.17253827232953	36.0	36.0	36.0	32.0	36.0
9	34.35157530691229	36.0	36.0	36.0	32.0	36.0
10	33.98386905917045	36.0	36.0	36.0	32.0	36.0
11	34.3499013115856	36.0	36.0	36.0	32.0	36.0
12	34.12918794660255	36.0	36.0	36.0	32.0	36.0
13	34.226361718677275	36.0	36.0	36.0	32.0	36.0
14	34.09732208080301	36.0	36.0	36.0	32.0	36.0
15	34.01972032760452	36.0	36.0	36.0	32.0	36.0
16	34.027822086325145	36.0	36.0	36.0	32.0	36.0
17	33.93885893788862	36.0	36.0	36.0	32.0	36.0
18	33.93673370536736	36.0	36.0	36.0	32.0	36.0
19	33.959650559392124	36.0	36.0	36.0	32.0	36.0
20	33.93147032326569	36.0	36.0	36.0	32.0	36.0
21	33.91021010929091	36.0	36.0	36.0	32.0	36.0
22	33.905806602262814	36.0	36.0	36.0	32.0	36.0
23	33.83805633522822	36.0	36.0	36.0	32.0	36.0
24	33.82247602999623	36.0	36.0	36.0	32.0	36.0
25	33.415199751346215	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	11.0
6	31.0
7	13.0
8	34.0
9	43.0
10	49.0
11	29.0
12	43.0
13	40.0
14	93.0
15	115.0
16	202.0
17	246.0
18	317.0
19	461.0
20	670.0
21	992.0
22	1641.0
23	2577.0
24	3724.0
25	5582.0
26	7757.0
27	10193.0
28	14115.0
29	18919.0
30	25133.0
31	33960.0
32	47784.0
33	66803.0
34	136454.0
35	255777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.291747359050724	17.33359218029638	11.474496471631793	25.900163989021102
2	16.396856953516995	20.22768152986841	37.87923254126038	25.496228975354224
3	18.551447211681825	24.68219720576518	28.85603616254211	27.910319420010886
4	12.430556103410463	15.597245122557336	36.3598065590066	35.6123922150256
5	14.335913708387112	37.024700279917575	33.54308742453745	15.096298587157875
6	33.20730600812655	35.89080437098111	17.4720896287822	13.429799992110143
7	29.736609538238174	30.656030047659627	21.336836789445442	18.270523624656757
8	28.083607964948165	32.58867128805384	19.898796671591096	19.428924075406897
9	27.932436165594538	13.831958640830342	18.673665101227357	39.56194009234776
10	15.668159112868086	26.853769742284644	31.987711000107353	25.490360144739917
11	36.7793064797493	21.09119325581946	23.083347146905364	19.046153117525872
12	24.692159156755288	23.81730539990495	29.1889485176747	22.301586925665063
13	29.624600538089403	19.703577157725878	25.419513951167755	25.25230835301696
14	23.513539117391645	19.55048551353912	25.949475013815427	30.98650035525381
15	25.00106569580463	27.176348184133285	22.986348042040518	24.836238078021562
16	25.169616903427706	25.72319089776565	24.41836046730108	24.68883173150556
17	23.609758100040892	25.72861290796244	25.748033112237728	24.913595879758937
18	24.33835697772837	25.074217713715434	26.74253717202087	23.844888136535328
19	25.262635976194197	24.935140401489996	25.918258746768863	23.883964875546944
20	25.712805871726523	24.105439684685255	25.584425018001745	24.59732942558648
21	26.790631868825955	24.095367726939692	24.87541370859756	24.238586695636798
22	25.705677157931138	24.370832063443437	25.352739882859566	24.57075089576586
23	24.274208830775063	24.431653085717894	25.831438317225697	25.46269976628135
24	24.494990967318113	24.896408403552435	25.908321437143904	24.700279191985548
25	24.6443409589411	24.583070803349646	25.845425503860177	24.927162733849077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	299.0
1	299.0
2	387.5
3	476.0
4	476.0
5	476.0
6	1196.0
7	1916.0
8	1916.0
9	1916.0
10	1820.5
11	1725.0
12	1725.0
13	1725.0
14	1727.5
15	1730.0
16	1730.0
17	1730.0
18	3013.0
19	4296.0
20	4296.0
21	4296.0
22	6846.5
23	9397.0
24	9397.0
25	9397.0
26	14188.0
27	18979.0
28	18979.0
29	18979.0
30	23847.5
31	28716.0
32	28716.0
33	28716.0
34	34682.5
35	40649.0
36	40649.0
37	40649.0
38	46870.0
39	53091.0
40	53091.0
41	53091.0
42	60550.5
43	68010.0
44	68010.0
45	68010.0
46	75859.5
47	83709.0
48	83709.0
49	83709.0
50	85710.0
51	87711.0
52	87711.0
53	87711.0
54	79888.5
55	72066.0
56	72066.0
57	72066.0
58	65609.5
59	59153.0
60	59153.0
61	59153.0
62	51722.0
63	44291.0
64	44291.0
65	44291.0
66	36151.0
67	28011.0
68	28011.0
69	28011.0
70	21073.0
71	14135.0
72	14135.0
73	14135.0
74	10807.5
75	7480.0
76	7480.0
77	7480.0
78	5942.5
79	4405.0
80	4405.0
81	4405.0
82	3067.0
83	1729.0
84	1729.0
85	1729.0
86	1315.5
87	902.0
88	902.0
89	902.0
90	644.0
91	386.0
92	386.0
93	386.0
94	286.0
95	186.0
96	186.0
97	186.0
98	275.5
99	365.0
100	365.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03691940682819696
2	0.005206583014232905
3	0.0012622019428443405
4	0.004417706799955192
5	0.00867763835705484
6	0.013884221371287747
7	0.024139612156898013
8	0.037392732556763585
9	0.05332803208517339
10	0.06326787238507257
11	0.05963904179939509
12	0.07099885928499415
13	0.07273438695640513
14	0.0730499374421162
15	0.06689670297075005
16	0.07478546511352717
17	0.07131440977070524
18	0.08535640638484854
19	0.08235867677059322
20	0.08535640638484854
21	0.0815698005563155
22	0.08772303502768167
23	0.09040521415622589
24	0.08788081027053721
25	0.0870919340562595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	633813.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.80271848346689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8870380898705	48.37264767849989
2	11.788240299000368	14.099376320331569
3	3.213305723027936	5.76493252666658
4	1.2965015824313444	3.1013727659004413
5	0.7093729615330973	2.12112157591735
6	0.45786185309433125	1.6428830102951257
7	0.28092135145529473	1.1759902347953235
8	0.20871155800461375	0.998521483807655
9	0.16351463281998876	0.880075759901512
>10	0.8435635054256035	9.05412093356716
>50	0.08012739417870413	3.3452403955504035
>100	0.06712653721730877	7.467324578576279
>500	0.0029185450963970915	1.124335174104676
>1k	7.95966844471934E-4	0.852057562086101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2193	0.34600110758220487	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1643	0.2592247240116564	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1534	0.2420272225404023	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	918	0.14483767294138808	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	814	0.12842904768441166	No Hit
GTACATGGGGTGGTATCAACGCAAA	768	0.12117138651305669	No Hit
GGTATCAACGCAGAGTACTTTTTTT	764	0.12054028554163451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.5777524285554256E-4
7	0.0	0.0	0.0	0.0	1.5777524285554256E-4
8	0.0	0.0	0.0	0.0	1.5777524285554256E-4
9	0.0	0.0	0.0	0.0	1.5777524285554256E-4
10	0.0	0.0	0.0	1.5777524285554256E-4	1.5777524285554256E-4
11	0.0	0.0	0.0	1.5777524285554256E-4	1.5777524285554256E-4
12	0.0	0.0	0.0	1.5777524285554256E-4	1.5777524285554256E-4
13	0.0	0.0	0.0	1.5777524285554256E-4	1.5777524285554256E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAGTC	25	0.0060367635	18.99534	3
ATTATAC	35	0.0021718447	16.281721	3
GTTTATA	45	6.771127E-4	14.772988	1
GCGTTAT	45	6.771127E-4	14.772988	1
TGCGAAT	40	0.0052692946	14.252133	19
AACCGCG	60	2.5637215E-5	14.249881	7
ACTTTGC	60	2.5637215E-5	14.249881	8
CTAATGT	40	0.005277586	14.248756	4
TTTAAAC	40	0.00528312	14.246506	3
CCAAGAC	60	2.569457E-5	14.246506	3
CGTTATT	40	0.0052942024	14.242008	2
CTAGAAC	55	1.9613419E-4	13.814794	3
TAAGACT	90	3.6294296E-8	13.7210245	4
ACCGTCG	135	1.8189894E-12	13.370258	8
AATTAAC	50	0.0015041835	13.292541	2
CTTAATC	65	5.4550794E-5	13.1506195	3
GTGCTAC	65	5.4591233E-5	13.149582	1
ATACCGT	145	0.0	13.103338	6
TCGCACG	75	1.4730396E-5	12.668563	18
CGCACGC	75	1.4730396E-5	12.668563	19
>>END_MODULE