FastQCFastQC Report
Thu 2 Feb 2017
SRR4064134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633813
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.11359817485599065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC551.3705358E-732.0038451
CTAGTAC300.005746250529.3299163
CCTAATA407.033206E-427.4967942
ACCGTCG1750.021.3689388
TAGGACG1259.949872E-1021.1175384
GATATAC1057.721792E-820.9548991
ATACCGT1700.020.7034686
TACCGTC1750.020.1119427
GCTTACT550.00448432219.997672
ATTATCC550.00448432219.997673
GTCCTAA1452.9467628E-1019.7265071
TAGGACA1452.9467628E-1019.721844
GTCCTAC1800.019.5579051
GTCTTAG1252.2795575E-819.3623261
TTAGGAC1504.656613E-1019.0644443
GTATCAA16950.018.952131
GTCGTAG1759.094947E-1218.85494611
CCGTCGT1901.8189894E-1218.5241579
CGTCGTA1901.8189894E-1218.52415710
GTATATC1203.1599484E-718.3311983
Produced by FastQC (version 0.11.5)