##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633813
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181103890264165	32.0	32.0	32.0	32.0	32.0
2	31.32012123449661	32.0	32.0	32.0	32.0	32.0
3	31.413218094295953	32.0	32.0	32.0	32.0	32.0
4	31.523643409018117	32.0	32.0	32.0	32.0	32.0
5	31.44614736523233	32.0	32.0	32.0	32.0	32.0
6	35.034951949549786	36.0	36.0	36.0	36.0	36.0
7	35.05574199330086	36.0	36.0	36.0	36.0	36.0
8	34.99928685590229	36.0	36.0	36.0	36.0	36.0
9	35.12867833256812	36.0	36.0	36.0	36.0	36.0
10	34.95316757466319	36.0	36.0	36.0	36.0	36.0
11	35.138047026488884	36.0	36.0	36.0	36.0	36.0
12	35.0311290554154	36.0	36.0	36.0	36.0	36.0
13	35.07254663441741	36.0	36.0	36.0	36.0	36.0
14	35.01488924966828	36.0	36.0	36.0	36.0	36.0
15	34.987371669561846	36.0	36.0	36.0	36.0	36.0
16	35.00328803606111	36.0	36.0	36.0	36.0	36.0
17	34.958189560643284	36.0	36.0	36.0	36.0	36.0
18	34.960709231271686	36.0	36.0	36.0	36.0	36.0
19	34.9585997762747	36.0	36.0	36.0	36.0	36.0
20	34.94822605405695	36.0	36.0	36.0	36.0	36.0
21	34.9545938628586	36.0	36.0	36.0	36.0	36.0
22	34.923224989074065	36.0	36.0	36.0	32.0	36.0
23	34.8706101010866	36.0	36.0	36.0	32.0	36.0
24	34.858567116799435	36.0	36.0	36.0	32.0	36.0
25	34.82901739156502	36.0	36.0	36.0	32.0	36.0
26	34.76042776023843	36.0	36.0	36.0	32.0	36.0
27	34.75329631926136	36.0	36.0	36.0	32.0	36.0
28	34.72830629854547	36.0	36.0	36.0	32.0	36.0
29	34.703649183592006	36.0	36.0	36.0	32.0	36.0
30	34.682564100136794	36.0	36.0	36.0	32.0	36.0
31	34.67371764226988	36.0	36.0	36.0	32.0	36.0
32	34.62262212987111	36.0	36.0	36.0	32.0	36.0
33	34.580395163873256	36.0	36.0	36.0	32.0	36.0
34	34.575762882743014	36.0	36.0	36.0	32.0	36.0
35	34.52557142248581	36.0	36.0	36.0	32.0	36.0
36	34.48782369563262	36.0	36.0	36.0	32.0	36.0
37	34.47831615949815	36.0	36.0	36.0	32.0	36.0
38	34.42420240670356	36.0	36.0	36.0	32.0	36.0
39	34.41847358763547	36.0	36.0	36.0	32.0	36.0
40	34.407337810994726	36.0	36.0	36.0	32.0	36.0
41	34.379870718966	36.0	36.0	36.0	32.0	36.0
42	34.30030781949881	36.0	36.0	36.0	32.0	36.0
43	34.296374482694425	36.0	36.0	36.0	32.0	36.0
44	34.21494983536154	36.0	36.0	36.0	32.0	36.0
45	34.168002234097436	36.0	36.0	36.0	32.0	36.0
46	34.13626574399704	36.0	36.0	36.0	32.0	36.0
47	34.089329186999954	36.0	36.0	36.0	32.0	36.0
48	34.04156115447301	36.0	36.0	36.0	32.0	36.0
49	34.00920934092548	36.0	36.0	36.0	32.0	36.0
50	33.39830202283639	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	12.0
21	30.0
22	87.0
23	209.0
24	621.0
25	1336.0
26	2782.0
27	4764.0
28	7902.0
29	12071.0
30	17207.0
31	24266.0
32	34736.0
33	56328.0
34	115042.0
35	356417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.314214483514924	18.456816908742336	12.084638385495973	26.14433022224677
2	15.663344435486287	21.00326438007545	37.33415797453815	25.99923320990011
3	17.8231646708004	25.229958504914723	29.24756630535965	27.69931051892523
4	12.16036906784809	16.440811406518957	36.39101753987375	35.00780198575921
5	13.963740093686939	37.41971212329189	33.348479756647464	15.26806802637371
6	33.304092229386995	36.22704560192234	17.131132151382666	13.337730017308
7	29.461102030255027	31.060629965983605	21.283440515484088	18.194827488277284
8	27.87340725641042	32.9047099139808	19.77889342581081	19.442989403797974
9	27.827170817522738	13.773005892979704	18.533776161437665	39.8660471280599
10	15.542999954244458	27.072292179115593	32.059961849344354	25.324746017295595
11	36.911833616539894	21.100387653771698	22.98580180589543	19.00197692379298
12	24.70981188457794	23.962903884899806	29.134460795218782	22.19282343530347
13	29.703048404324978	19.68217454209247	25.515811595643466	25.098965457939087
14	23.554776495238336	19.562741002063703	25.93750197219365	30.944980530504314
15	25.016685574354547	27.34177894256849	22.85726457561839	24.78427090745857
16	25.221557982161762	25.681950499126703	24.35851923377306	24.737972284938476
17	23.582349999132234	25.857311225866304	25.780474682595656	24.779864092405806
18	24.273286084832726	24.97838475762529	26.963673770771145	23.78465538677084
19	25.330973015700213	24.813785769619745	26.061945715849944	23.793295498830098
20	25.886072753893924	24.14800696740969	25.640414762830382	24.325505515866002
21	26.702197651357736	24.058200131584552	25.006429341146365	24.23317287591135
22	25.687229514068026	24.360024171167204	25.554856085312228	24.397890229452535
23	24.312405727953752	24.34822121665373	25.870458375144366	25.46891468024815
24	24.669577112347728	24.707601185846208	25.997264160022972	24.625557541783092
25	24.61920284252811	24.428449263334606	26.135449241245688	24.8168986528916
26	24.336243390834973	25.073566653360242	26.3574526767217	24.23273727908309
27	24.880517910874016	24.848961069903137	25.63535754689741	24.635163472325438
28	24.216495447208604	24.84399332898908	26.225056683081228	24.714454540721096
29	24.355112789191423	24.862060641250377	25.956108697950274	24.826717871607922
30	24.254052203328857	25.221405794654707	26.087779725493103	24.436762276523332
31	24.90118577075099	24.833337278013143	25.29549596459255	24.969980986643314
32	24.532573880938482	25.078338250840183	25.401000331340036	24.988087536881302
33	24.39770584661991	24.63942819725932	25.779089123283132	25.183776832837633
34	24.915034948484514	24.7690875526594	25.94502911058868	24.370848388267408
35	25.315638835331356	24.658958589068373	25.93082294878147	24.094579626818806
36	24.4141700551787	25.23782699732864	25.578334982225105	24.769667965267555
37	25.01830253458548	25.204956831263253	25.34191027971322	24.43483035443805
38	24.5129457727622	25.144731672449623	25.635770064014867	24.706552490773305
39	24.99404374024712	24.99025697907972	25.117586823333394	24.898112457339767
40	25.17201717252837	25.12799718524526	25.639512593029696	24.060473049196673
41	24.199813818457216	25.41772511399675	25.966645103267645	24.41581596427839
42	25.151980359703945	25.71383040995647	25.504931374142277	23.62925785619731
43	24.606422826056086	24.747160785229458	25.70597757659332	24.940438812121137
44	24.406952949102482	25.443394871908147	25.147403846305554	25.002248332683813
45	24.44387644819322	25.56014685858629	25.18889819771634	24.80707849550415
46	24.3758746747827	25.19852257863987	25.21382692207565	25.211775824501782
47	24.55407646296843	25.207440237173735	25.519685325111986	24.718797974745858
48	25.0640984173016	25.550851936995002	24.857247894021846	24.527801751681547
49	24.578266110455466	25.697841000170396	24.85847538386777	24.865417505506365
50	24.503396938495374	26.007958271142904	24.802699879773936	24.685944910587782
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	124.0
1	86.5
2	49.0
3	124.0
4	199.0
5	188.0
6	177.0
7	190.0
8	203.0
9	266.0
10	329.0
11	483.0
12	637.0
13	1144.0
14	1651.0
15	2082.0
16	2513.0
17	2666.0
18	2819.0
19	2786.5
20	2754.0
21	2923.5
22	3093.0
23	3364.0
24	3635.0
25	3857.0
26	4079.0
27	4868.0
28	5657.0
29	6796.5
30	7936.0
31	9185.5
32	10435.0
33	12477.0
34	14519.0
35	16663.5
36	18808.0
37	21554.5
38	24301.0
39	26022.5
40	27744.0
41	30498.5
42	33253.0
43	34365.0
44	35477.0
45	38676.0
46	41875.0
47	44146.0
48	46417.0
49	47993.0
50	49569.0
51	47806.0
52	46043.0
53	45517.0
54	44991.0
55	44464.5
56	43938.0
57	42389.0
58	40840.0
59	37287.5
60	33735.0
61	29560.0
62	25385.0
63	22219.5
64	19054.0
65	16109.0
66	13164.0
67	11286.0
68	9408.0
69	8321.0
70	7234.0
71	5681.0
72	4128.0
73	3485.0
74	2842.0
75	2208.5
76	1575.0
77	1422.0
78	1269.0
79	1043.5
80	818.0
81	648.5
82	479.0
83	401.5
84	324.0
85	229.0
86	134.0
87	114.0
88	94.0
89	65.5
90	37.0
91	29.0
92	21.0
93	15.5
94	10.0
95	15.0
96	20.0
97	14.0
98	8.0
99	8.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00915096408562147
2	3.155504857110851E-4
3	4.733257285666277E-4
4	0.0
5	0.0
6	3.155504857110851E-4
7	1.5777524285554256E-4
8	1.5777524285554256E-4
9	0.0012622019428443405
10	0.0015777524285554255
11	0.0
12	0.0
13	9.466514571332554E-4
14	1.5777524285554256E-4
15	0.005048807771377362
16	9.466514571332554E-4
17	0.0
18	1.5777524285554256E-4
19	0.0
20	7.888762142777127E-4
21	0.0
22	0.0
23	0.001419977185699883
24	6.311009714221702E-4
25	0.0017355276714109684
26	0.005364358257088447
27	0.005679908742799532
28	0.0041021563142441065
29	0.003155504857110851
30	0.003471055342821937
31	0.007573211657066043
32	0.003628830585677479
33	0.004417706799955192
34	0.003628830585677479
35	0.0029977296142553087
36	0.008519863114199298
37	0.0033132800999663937
38	0.008519863114199298
39	0.0041021563142441065
40	0.0015777524285554255
41	0.003628830585677479
42	0.002208853399977596
43	0.0017355276714109684
44	0.0015777524285554255
45	9.466514571332554E-4
46	9.466514571332554E-4
47	0.002524403885688681
48	0.003471055342821937
49	1.5777524285554256E-4
50	0.001104426699988798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	633813.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.773876098035984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.95632721116787	52.26672657015114
2	11.441371093699034	14.593211650424253
3	2.944698337038821	5.633844806772192
4	1.2117614383523783	3.091148953194498
5	0.6326674116553236	2.0173826561085866
6	0.3836372053117672	1.467961895888966
7	0.2640626391339429	1.1788208621173923
8	0.18536309276518084	0.9457058328924314
9	0.14016468882328315	0.8044960948502244
>10	0.7183745048907985	8.213953361921963
>50	0.06975469232681766	3.1290610017624525
>100	0.050821190895682856	6.277496757798344
>500	9.964939391199823E-4	0.38018955611768657
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	720	0.11359817485599065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.5777524285554256E-4
7	0.0	0.0	0.0	0.0	1.5777524285554256E-4
8	0.0	0.0	0.0	0.0	1.5777524285554256E-4
9	0.0	0.0	0.0	0.0	1.5777524285554256E-4
10	0.0	0.0	0.0	0.0	1.5777524285554256E-4
11	0.0	0.0	0.0	0.0	1.5777524285554256E-4
12	0.0	0.0	0.0	0.0	4.733257285666277E-4
13	0.0	0.0	0.0	0.0	4.733257285666277E-4
14	0.0	0.0	0.0	0.0	4.733257285666277E-4
15	0.0	0.0	0.0	0.0	4.733257285666277E-4
16	0.0	0.0	0.0	0.0	4.733257285666277E-4
17	0.0	0.0	0.0	0.0	6.311009714221702E-4
18	0.0	0.0	0.0	0.0	6.311009714221702E-4
19	0.0	0.0	0.0	0.0	6.311009714221702E-4
20	0.0	0.0	0.0	1.5777524285554256E-4	6.311009714221702E-4
21	0.0	0.0	0.0	1.5777524285554256E-4	6.311009714221702E-4
22	0.0	0.0	0.0	6.311009714221702E-4	6.311009714221702E-4
23	0.0	0.0	0.0	0.0015777524285554257	6.311009714221702E-4
24	0.0	0.0	0.0	0.0033132800999663937	6.311009714221702E-4
25	0.0	0.0	0.0	0.0037866058285330214	6.311009714221702E-4
26	0.0	0.0	0.0	0.004417706799955192	6.311009714221702E-4
27	0.0	0.0	0.0	0.006311009714221703	6.311009714221702E-4
28	0.0	0.0	0.0	0.008046537385632671	6.311009714221702E-4
29	0.0	0.0	0.0	0.014988648071276544	6.311009714221702E-4
30	0.0	0.0	0.0	0.028399543713997662	6.311009714221702E-4
31	0.0	0.0	0.0	0.05774573888512858	6.311009714221702E-4
32	0.0	0.0	0.0	0.08661860832769287	6.311009714221702E-4
33	0.0	0.0	0.0	0.11722700544166813	6.311009714221702E-4
34	0.0	0.0	0.0	0.14467989769853254	6.311009714221702E-4
35	0.0	0.0	0.0	0.18175707976958502	6.311009714221702E-4
36	0.0	0.0	0.0	0.22735412495483684	6.311009714221702E-4
37	0.0	0.0	0.0	0.30750394832545247	6.311009714221702E-4
38	0.0	0.0	0.0	0.40343129598162236	6.311009714221702E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	55	1.3705358E-7	32.003845	1
CTAGTAC	30	0.0057462505	29.329916	3
CCTAATA	40	7.033206E-4	27.496794	2
ACCGTCG	175	0.0	21.368938	8
TAGGACG	125	9.949872E-10	21.117538	4
GATATAC	105	7.721792E-8	20.954899	1
ATACCGT	170	0.0	20.703468	6
TACCGTC	175	0.0	20.111942	7
GCTTACT	55	0.004484322	19.99767	2
ATTATCC	55	0.004484322	19.99767	3
GTCCTAA	145	2.9467628E-10	19.726507	1
TAGGACA	145	2.9467628E-10	19.72184	4
GTCCTAC	180	0.0	19.557905	1
GTCTTAG	125	2.2795575E-8	19.362326	1
TTAGGAC	150	4.656613E-10	19.064444	3
GTATCAA	1695	0.0	18.95213	1
GTCGTAG	175	9.094947E-12	18.854946	11
CCGTCGT	190	1.8189894E-12	18.524157	9
CGTCGTA	190	1.8189894E-12	18.524157	10
GTATATC	120	3.1599484E-7	18.331198	3
>>END_MODULE