FastQCFastQC Report
Thu 2 Feb 2017
SRR4064133_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064133_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3620
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT421.1602209944751383No Hit
TATCAACGCAGAGTACTTTTTTTTT381.0497237569060773No Hit
GGTATCAACGCAGAGTACTTTTTTT180.49723756906077343No Hit
ACGCAGAGTACTTTTTTTTTTTTTT110.30386740331491713No Hit
GTACTTTTTTTTTTTTTTTTTTTTT100.2762430939226519No Hit
GAGTACTTTTTTTTTTTTTTTTTTT80.22099447513812157No Hit
CTGTAGGACGTGGAATATGGCAAGA80.22099447513812157No Hit
GTCCTAAAGTGTGTATTTCTCATTT60.16574585635359115No Hit
CTATTGGAGCTGGAATTACCGCGGC60.16574585635359115No Hit
GTCCTACAGTGGACATTTCTAAATT50.13812154696132595No Hit
GTATCAACGCAGAGTACATGGGCAG50.13812154696132595No Hit
ATTTAGAAATGTCCACTGTAGGACG50.13812154696132595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG40.11049723756906078No Hit
GATTAAGAGGGACGGCCGGGGGCAT40.11049723756906078No Hit
TTCCAGGTCCTTCAGTGTGCATTTC40.11049723756906078No Hit
ATCAACGCAGAGTACTTTTTTTTTT40.11049723756906078No Hit
CTGTAGGACATGGAATATGGCAAGA40.11049723756906078No Hit
GTATCAACGCAGAGTACATGGGAGT40.11049723756906078No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT40.11049723756906078No Hit
GAAATATGGCGAGGAAAACTGAAAA40.11049723756906078No Hit
GTATTAGCTCTAGAATTACCACAGT40.11049723756906078No Hit
CATCTAATATGTTCTACAGTGTGGT40.11049723756906078No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)