##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064133_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3620
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.4853591160221	32.0	32.0	32.0	14.0	32.0
2	26.664088397790056	32.0	14.0	32.0	14.0	32.0
3	27.106077348066297	32.0	21.0	32.0	14.0	32.0
4	26.677071823204418	32.0	21.0	32.0	14.0	32.0
5	26.869337016574587	32.0	21.0	32.0	14.0	32.0
6	30.25414364640884	36.0	21.0	36.0	14.0	36.0
7	30.028729281767955	36.0	21.0	36.0	14.0	36.0
8	30.039226519337017	36.0	21.0	36.0	14.0	36.0
9	30.25220994475138	36.0	21.0	36.0	14.0	36.0
10	28.900276243093924	36.0	21.0	36.0	14.0	36.0
11	30.608839779005525	36.0	21.0	36.0	14.0	36.0
12	29.746408839779004	36.0	21.0	36.0	14.0	36.0
13	30.4232044198895	36.0	21.0	36.0	14.0	36.0
14	29.457734806629833	36.0	21.0	36.0	14.0	36.0
15	29.468508287292817	36.0	21.0	36.0	14.0	36.0
16	29.630939226519338	36.0	21.0	36.0	14.0	36.0
17	29.516022099447515	36.0	21.0	36.0	14.0	36.0
18	29.20331491712707	36.0	21.0	36.0	14.0	36.0
19	29.425690607734808	36.0	21.0	36.0	14.0	36.0
20	29.184806629834256	36.0	21.0	36.0	14.0	36.0
21	29.046132596685084	36.0	21.0	36.0	14.0	36.0
22	29.173756906077347	36.0	21.0	36.0	14.0	36.0
23	28.840883977900553	36.0	21.0	36.0	14.0	36.0
24	29.040055248618785	36.0	21.0	36.0	14.0	36.0
25	28.400828729281766	36.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	16.0
16	28.0
17	72.0
18	103.0
19	111.0
20	144.0
21	135.0
22	112.0
23	111.0
24	131.0
25	126.0
26	133.0
27	138.0
28	145.0
29	189.0
30	176.0
31	176.0
32	253.0
33	268.0
34	453.0
35	596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94528875379939	19.204200055263883	14.03702680298425	24.81348438795247
2	14.502762430939226	20.0	41.68508287292818	23.8121546961326
3	17.707182320441987	25.16574585635359	29.530386740331494	27.59668508287293
4	12.762430939226519	15.6353591160221	35.88397790055249	35.71823204419889
5	12.596685082872927	37.07182320441989	35.27624309392265	15.05524861878453
6	33.17679558011049	37.67955801104972	16.243093922651934	12.900552486187845
7	27.127071823204417	31.933701657458563	21.132596685082873	19.806629834254146
8	27.209944751381215	34.69613259668508	19.281767955801104	18.812154696132595
9	27.375690607734803	13.342541436464089	19.917127071823202	39.364640883977906
10	15.27624309392265	27.265193370165747	31.933701657458563	25.524861878453038
11	36.32596685082873	20.994475138121548	24.171270718232044	18.50828729281768
12	25.13812154696133	25.41436464088398	29.917127071823202	19.530386740331494
13	30.754352030947775	21.055540204476376	24.48190107764576	23.70820668693009
14	21.685082872928177	21.353591160220994	26.933701657458563	30.027624309392266
15	23.480662983425415	29.337016574585633	24.005524861878452	23.1767955801105
16	23.535911602209943	26.32596685082873	26.49171270718232	23.646408839779006
17	21.353591160220994	26.712707182320443	28.45303867403315	23.480662983425415
18	21.8568665377176	25.338491295938105	30.892511743575575	21.912130422768723
19	24.34374136501796	23.901630284609006	29.455650732246475	22.298977618126553
20	23.376623376623375	25.44901906604034	28.73722022658193	22.437137330754354
21	24.696132596685082	24.392265193370164	26.988950276243095	23.92265193370166
22	25.11743575573363	25.006907985631386	26.66482453716496	23.21083172147002
23	21.663442940038685	24.37137330754352	29.897761812655432	24.067421939762365
24	24.509533020171318	24.177949709864606	28.460900801326332	22.851616468637744
25	22.409505388228794	25.034539928156953	27.631942525559545	24.924012158054712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	4.5
3	9.0
4	9.0
5	9.0
6	11.0
7	13.0
8	13.0
9	13.0
10	13.0
11	13.0
12	13.0
13	13.0
14	11.0
15	9.0
16	9.0
17	9.0
18	18.0
19	27.0
20	27.0
21	27.0
22	49.5
23	72.0
24	72.0
25	72.0
26	131.5
27	191.0
28	191.0
29	191.0
30	235.0
31	279.0
32	279.0
33	279.0
34	284.5
35	290.0
36	290.0
37	290.0
38	298.5
39	307.0
40	307.0
41	307.0
42	337.5
43	368.0
44	368.0
45	368.0
46	407.0
47	446.0
48	446.0
49	446.0
50	453.5
51	461.0
52	461.0
53	461.0
54	416.0
55	371.0
56	371.0
57	371.0
58	322.0
59	273.0
60	273.0
61	273.0
62	230.0
63	187.0
64	187.0
65	187.0
66	165.5
67	144.0
68	144.0
69	144.0
70	110.5
71	77.0
72	77.0
73	77.0
74	54.5
75	32.0
76	32.0
77	32.0
78	33.0
79	34.0
80	34.0
81	34.0
82	20.0
83	6.0
84	6.0
85	6.0
86	4.0
87	2.0
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	3.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027624309392265196
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.027624309392265196
14	0.0
15	0.0
16	0.0
17	0.0
18	0.027624309392265196
19	0.027624309392265196
20	0.027624309392265196
21	0.0
22	0.027624309392265196
23	0.027624309392265196
24	0.027624309392265196
25	0.027624309392265196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.4696132596685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.20610687022901	86.13259668508287
2	3.2061068702290076	5.801104972375691
3	0.9160305343511451	2.4861878453038675
4	0.3053435114503817	1.1049723756906076
5	0.0916030534351145	0.4143646408839779
6	0.06106870229007634	0.3314917127071823
7	0.0	0.0
8	0.06106870229007634	0.44198895027624313
9	0.0	0.0
>10	0.15267175572519084	3.287292817679558
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	42	1.1602209944751383	No Hit
TATCAACGCAGAGTACTTTTTTTTT	38	1.0497237569060773	No Hit
GGTATCAACGCAGAGTACTTTTTTT	18	0.49723756906077343	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	11	0.30386740331491713	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	10	0.2762430939226519	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8	0.22099447513812157	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8	0.22099447513812157	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6	0.16574585635359115	No Hit
CTATTGGAGCTGGAATTACCGCGGC	6	0.16574585635359115	No Hit
GTCCTACAGTGGACATTTCTAAATT	5	0.13812154696132595	No Hit
GTATCAACGCAGAGTACATGGGCAG	5	0.13812154696132595	No Hit
ATTTAGAAATGTCCACTGTAGGACG	5	0.13812154696132595	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	4	0.11049723756906078	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	4	0.11049723756906078	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	4	0.11049723756906078	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	4	0.11049723756906078	No Hit
CTGTAGGACATGGAATATGGCAAGA	4	0.11049723756906078	No Hit
GTATCAACGCAGAGTACATGGGAGT	4	0.11049723756906078	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	4	0.11049723756906078	No Hit
GAAATATGGCGAGGAAAACTGAAAA	4	0.11049723756906078	No Hit
GTATTAGCTCTAGAATTACCACAGT	4	0.11049723756906078	No Hit
CATCTAATATGTTCTACAGTGTGGT	4	0.11049723756906078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE