##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3620
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.585082872928176	32.0	32.0	32.0	21.0	32.0
2	29.61243093922652	32.0	32.0	32.0	14.0	32.0
3	30.579005524861877	32.0	32.0	32.0	32.0	32.0
4	31.097513812154695	32.0	32.0	32.0	32.0	32.0
5	30.736187845303867	32.0	32.0	32.0	32.0	32.0
6	34.274033149171274	36.0	36.0	36.0	32.0	36.0
7	34.22209944751381	36.0	36.0	36.0	32.0	36.0
8	34.00082872928177	36.0	36.0	36.0	32.0	36.0
9	34.57292817679558	36.0	36.0	36.0	32.0	36.0
10	34.13756906077348	36.0	36.0	36.0	32.0	36.0
11	34.658839779005525	36.0	36.0	36.0	32.0	36.0
12	34.41657458563536	36.0	36.0	36.0	32.0	36.0
13	34.611049723756906	36.0	36.0	36.0	32.0	36.0
14	34.28591160220994	36.0	36.0	36.0	32.0	36.0
15	34.22983425414365	36.0	36.0	36.0	32.0	36.0
16	34.2	36.0	36.0	36.0	32.0	36.0
17	34.212983425414365	36.0	36.0	36.0	32.0	36.0
18	34.327900552486184	36.0	36.0	36.0	32.0	36.0
19	34.31325966850829	36.0	36.0	36.0	32.0	36.0
20	34.25	36.0	36.0	36.0	32.0	36.0
21	34.26906077348066	36.0	36.0	36.0	32.0	36.0
22	34.24972375690608	36.0	36.0	36.0	32.0	36.0
23	34.036740331491714	36.0	36.0	36.0	32.0	36.0
24	34.20303867403315	36.0	36.0	36.0	32.0	36.0
25	34.209668508287294	36.0	36.0	36.0	32.0	36.0
26	33.77790055248619	36.0	36.0	36.0	32.0	36.0
27	33.91546961325967	36.0	36.0	36.0	32.0	36.0
28	33.91933701657459	36.0	36.0	36.0	32.0	36.0
29	33.8914364640884	36.0	36.0	36.0	32.0	36.0
30	33.82762430939226	36.0	36.0	36.0	32.0	36.0
31	33.764088397790054	36.0	36.0	36.0	32.0	36.0
32	33.67817679558011	36.0	36.0	36.0	27.0	36.0
33	33.67624309392265	36.0	36.0	36.0	27.0	36.0
34	33.68895027624309	36.0	36.0	36.0	32.0	36.0
35	33.572375690607736	36.0	36.0	36.0	21.0	36.0
36	33.24751381215469	36.0	36.0	36.0	21.0	36.0
37	33.37872928176795	36.0	36.0	36.0	21.0	36.0
38	33.434254143646406	36.0	36.0	36.0	21.0	36.0
39	33.56823204419889	36.0	36.0	36.0	21.0	36.0
40	33.430939226519335	36.0	36.0	36.0	21.0	36.0
41	33.35966850828729	36.0	36.0	36.0	21.0	36.0
42	33.32265193370166	36.0	36.0	36.0	21.0	36.0
43	33.18812154696133	36.0	36.0	36.0	21.0	36.0
44	33.277348066298345	36.0	36.0	36.0	21.0	36.0
45	32.96049723756906	36.0	36.0	36.0	14.0	36.0
46	32.918232044198895	36.0	36.0	36.0	14.0	36.0
47	32.988950276243095	36.0	36.0	36.0	14.0	36.0
48	32.99751381215469	36.0	36.0	36.0	14.0	36.0
49	32.79696132596685	36.0	36.0	36.0	14.0	36.0
50	31.85441988950276	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	4.0
24	8.0
25	18.0
26	18.0
27	44.0
28	74.0
29	134.0
30	195.0
31	283.0
32	408.0
33	544.0
34	794.0
35	1095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51933701657459	20.801104972375693	13.066298342541435	24.613259668508285
2	16.022099447513813	21.85082872928177	38.370165745856355	23.756906077348066
3	21.2707182320442	23.8121546961326	30.193370165745858	24.723756906077348
4	15.248618784530388	17.34806629834254	36.38121546961326	31.022099447513813
5	17.87292817679558	35.469613259668506	32.09944751381216	14.558011049723756
6	34.64088397790056	37.348066298342545	14.944751381215472	13.066298342541435
7	29.64088397790055	30.82872928176796	20.05524861878453	19.475138121546962
8	27.40331491712707	35.165745856353595	18.39779005524862	19.03314917127072
9	26.353591160220997	13.563535911602209	18.23204419889503	41.85082872928177
10	15.497237569060774	28.14917127071823	31.574585635359114	24.779005524861876
11	37.23756906077348	20.828729281767956	22.40331491712707	19.530386740331494
12	24.116022099447516	23.314917127071823	30.607734806629832	21.96132596685083
13	29.806629834254146	20.248618784530386	24.19889502762431	25.74585635359116
14	22.734806629834253	20.96685082872928	24.88950276243094	31.408839779005525
15	25.193370165745854	29.806629834254146	22.4585635359116	22.5414364640884
16	24.558011049723756	26.10497237569061	25.0	24.337016574585636
17	22.29281767955801	26.574585635359117	26.32596685082873	24.806629834254142
18	22.40331491712707	26.215469613259668	28.784530386740332	22.596685082872927
19	24.944751381215468	25.41436464088398	26.629834254143645	23.01104972375691
20	25.856353591160218	26.464088397790054	25.41436464088398	22.265193370165743
21	25.58011049723757	24.585635359116022	24.226519337016576	25.607734806629836
22	23.287292817679557	26.76795580110497	25.91160220994475	24.03314917127072
23	24.53038674033149	26.022099447513813	25.110497237569064	24.337016574585636
24	24.392265193370164	26.74033149171271	25.386740331491715	23.480662983425415
25	23.480662983425415	26.96132596685083	25.16574585635359	24.392265193370164
26	23.259668508287294	29.005524861878452	26.10497237569061	21.629834254143645
27	24.03314917127072	26.850828729281766	26.381215469613263	22.734806629834253
28	23.204419889502763	28.011049723756905	25.524861878453038	23.259668508287294
29	23.67403314917127	25.662983425414364	26.13259668508287	24.53038674033149
30	22.955801104972377	27.09944751381215	26.1878453038674	23.756906077348066
31	24.005524861878452	27.09944751381215	24.834254143646408	24.060773480662984
32	22.430939226519335	27.486187845303867	25.635359116022098	24.447513812154696
33	22.486187845303867	27.154696132596683	26.602209944751383	23.756906077348066
34	23.259668508287294	27.59668508287293	26.243093922651934	22.900552486187845
35	24.392265193370164	28.425414364640883	24.779005524861876	22.40331491712707
36	23.61878453038674	26.906077348066297	26.160220994475136	23.314917127071823
37	22.900552486187845	27.40331491712707	25.46961325966851	24.226519337016576
38	22.955801104972377	27.81767955801105	25.96685082872928	23.259668508287294
39	23.92265193370166	27.23756906077348	24.88950276243094	23.950276243093924
40	24.19889502762431	27.430939226519335	24.806629834254142	23.56353591160221
41	22.76243093922652	26.712707182320443	26.906077348066297	23.61878453038674
42	24.364640883977902	28.259668508287294	25.91160220994475	21.464088397790054
43	22.845303867403317	28.535911602209946	25.41436464088398	23.204419889502763
44	24.03314917127072	27.569060773480665	24.668508287292816	23.7292817679558
45	22.734806629834253	26.49171270718232	26.574585635359117	24.19889502762431
46	22.65193370165746	27.01657458563536	25.74585635359116	24.585635359116022
47	24.475138121546962	28.342541436464085	24.613259668508285	22.56906077348066
48	24.19889502762431	26.712707182320443	24.41988950276243	24.668508287292816
49	23.950276243093924	25.552486187845304	26.54696132596685	23.950276243093924
50	23.591160220994475	27.76243093922652	24.806629834254142	23.83977900552486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	3.0
7	3.0
8	3.0
9	5.0
10	7.0
11	6.5
12	6.0
13	7.0
14	8.0
15	10.5
16	13.0
17	13.5
18	14.0
19	17.0
20	20.0
21	22.5
22	25.0
23	25.5
24	26.0
25	30.0
26	34.0
27	45.0
28	56.0
29	61.5
30	67.0
31	81.5
32	96.0
33	119.5
34	143.0
35	155.0
36	167.0
37	189.0
38	211.0
39	201.5
40	192.0
41	181.0
42	170.0
43	190.0
44	210.0
45	199.0
46	188.0
47	208.0
48	228.0
49	225.0
50	222.0
51	208.5
52	195.0
53	210.0
54	225.0
55	227.5
56	230.0
57	200.5
58	171.0
59	172.5
60	174.0
61	154.5
62	135.0
63	115.5
64	96.0
65	86.5
66	77.0
67	62.0
68	47.0
69	49.0
70	51.0
71	37.5
72	24.0
73	21.0
74	18.0
75	20.5
76	23.0
77	14.5
78	6.0
79	8.0
80	10.0
81	10.5
82	11.0
83	7.5
84	4.0
85	3.5
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.65745856353591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.45641611889111	94.1988950276243
2	1.886253215204344	3.6464088397790055
3	0.48585310088596745	1.408839779005525
4	0.11431837667905116	0.44198895027624313
5	0.02857959416976279	0.13812154696132595
6	0.02857959416976279	0.16574585635359115
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	6	0.16574585635359115	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	5	0.13812154696132595	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	4	0.11049723756906078	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	4	0.11049723756906078	No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC	4	0.11049723756906078	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	4	0.11049723756906078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.027624309392265192	0.0
30	0.0	0.0	0.0	0.027624309392265192	0.0
31	0.0	0.0	0.0	0.027624309392265192	0.0
32	0.0	0.0	0.0	0.027624309392265192	0.0
33	0.0	0.0	0.0	0.055248618784530384	0.0
34	0.0	0.0	0.0	0.08287292817679558	0.0
35	0.0	0.0	0.0	0.11049723756906077	0.0
36	0.0	0.0	0.0	0.11049723756906077	0.0
37	0.0	0.0	0.0	0.16574585635359115	0.0
38	0.0	0.0	0.0	0.16574585635359115	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE