FastQCFastQC Report
Thu 2 Feb 2017
SRR4064132_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064132_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874975
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT20020.22880653732963802No Hit
CTTTAGGACGTGAAATATGGCGAGG19370.2213777536501043No Hit
CTGTAGGACGTGGAATATGGCAAGA18950.21657761650332868No Hit
GTCCTACAGTGGACATTTCTAAATT18250.20857738792536928No Hit
GTATCAACGCAGAGTACTTTTTTTT12020.13737535358153088No Hit
CTGAAGGACCTGGAATATGGCGAGA10410.11897482785222435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA250.006023614719.00349210
ACTTCCG250.006025606319.00240315
GCTCGCG250.006025606319.00240313
GGTCGTG307.7154377E-419.0013167
TAGCGAA601.4622146E-615.83624310
GCGAAAT551.1252199E-515.5483112
GTAGCGA551.1252199E-515.548319
GGTATCA3000.014.8801081
TAACGAT400.005284252614.2469114
GTATCAA11200.014.0773061
TCCAACG759.651212E-713.934298518
TATTCCG551.962213E-413.81518655
TAGGACC3200.013.356484
GTTCGTT500.001497849113.30092119
ACACCGC500.001499141613.29946
TGTACCG500.001501082113.2971175
GGTAGCG655.4407017E-513.155518
GATCTAT604.0963283E-412.663921
CGTAAAT853.957408E-612.288643
AGCGAAA701.0879787E-412.2165311
Produced by FastQC (version 0.11.5)