##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064132_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874975
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37737535358153	32.0	32.0	32.0	32.0	32.0
2	31.102113774679278	32.0	32.0	32.0	32.0	32.0
3	31.100760593159805	32.0	32.0	32.0	32.0	32.0
4	31.125972742078346	32.0	32.0	32.0	32.0	32.0
5	31.10424983570959	32.0	32.0	32.0	32.0	32.0
6	34.77020372010629	36.0	36.0	36.0	36.0	36.0
7	34.718147375639305	36.0	36.0	36.0	32.0	36.0
8	34.687017343352665	36.0	36.0	36.0	32.0	36.0
9	34.76893968399097	36.0	36.0	36.0	32.0	36.0
10	34.573844966999054	36.0	36.0	36.0	32.0	36.0
11	34.75253235806737	36.0	36.0	36.0	36.0	36.0
12	34.632658075945024	36.0	36.0	36.0	32.0	36.0
13	34.66839509700277	36.0	36.0	36.0	32.0	36.0
14	34.617896511328894	36.0	36.0	36.0	32.0	36.0
15	34.583202948655675	36.0	36.0	36.0	32.0	36.0
16	34.568299665704735	36.0	36.0	36.0	32.0	36.0
17	34.54932426640761	36.0	36.0	36.0	32.0	36.0
18	34.532474642132634	36.0	36.0	36.0	32.0	36.0
19	34.51452670076288	36.0	36.0	36.0	32.0	36.0
20	34.50351038601103	36.0	36.0	36.0	32.0	36.0
21	34.47823309237407	36.0	36.0	36.0	32.0	36.0
22	34.459434840995456	36.0	36.0	36.0	32.0	36.0
23	34.43463641818337	36.0	36.0	36.0	32.0	36.0
24	34.40593159804566	36.0	36.0	36.0	32.0	36.0
25	34.05864967570502	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	10.0
5	17.0
6	38.0
7	15.0
8	50.0
9	61.0
10	84.0
11	24.0
12	51.0
13	51.0
14	55.0
15	130.0
16	196.0
17	260.0
18	350.0
19	420.0
20	569.0
21	754.0
22	1241.0
23	1883.0
24	3061.0
25	4567.0
26	6809.0
27	9417.0
28	13240.0
29	18807.0
30	25449.0
31	35471.0
32	50647.0
33	74279.0
34	172521.0
35	454445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01501675579078	20.273306327411923	12.345890743783322	25.365786173013973
2	13.866690135371758	21.44266244839552	42.317618444573476	22.373028971659252
3	17.703444981107577	25.81564287951138	30.86322252369839	25.617689615682654
4	11.30815634693677	17.533711422451727	39.87788681557069	31.280245415040813
5	12.166139546154382	38.620312066011415	36.38937498785547	12.824173399978738
6	29.679005628432044	39.63906707938787	18.72368102198792	11.958246270192172
7	26.881138995079656	32.812185619432604	22.89685116423698	17.409824221250755
8	24.141743518914456	38.06661620677275	21.14341967158384	16.64822060272895
9	26.181162013907393	15.432836401185135	21.033757614359764	37.35224397054771
10	14.547305101609048	29.243621559187126	34.84269752867583	21.366375810528
11	33.36009267423019	23.05431574421272	25.716870163449258	17.868721418107842
12	23.019572681047872	25.717135073686332	32.291607100786614	18.971685144479185
13	28.05253087612837	23.060999724376444	26.81474094244957	22.071728457045616
14	20.356158429883454	22.80283188269875	28.376585499754096	28.464424187663695
15	22.36761300283043	32.07936644080396	23.778368642250623	21.77465191411499
16	21.486427445678903	29.370220669809616	27.958867165787144	21.184484718724338
17	20.379062362758017	29.473859976577366	28.62101540770019	21.526062252964426
18	20.380340604550774	28.590513903615477	30.88923432032933	20.139911171504423
19	23.0431018015988	27.004994973000613	28.08188232818128	21.87002089721931
20	23.419626179983094	28.09883891836742	28.158317043571156	20.32321785807833
21	23.411668088785998	27.183677577715198	26.902189453746043	22.502464879752758
22	22.709439953150234	29.213390614491402	27.54402459612666	20.533144836231703
23	21.604327499962825	29.051054479234708	28.363243703265645	20.98137431753683
24	22.481610561388297	27.55424755712993	28.71749178465913	21.246650096822638
25	22.110825788814477	28.142475593505328	28.473500294536493	21.273198323143706
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	50.0
2	141.5
3	233.0
4	233.0
5	233.0
6	662.5
7	1092.0
8	1092.0
9	1092.0
10	1469.0
11	1846.0
12	1846.0
13	1846.0
14	3154.0
15	4462.0
16	4462.0
17	4462.0
18	7557.0
19	10652.0
20	10652.0
21	10652.0
22	17259.5
23	23867.0
24	23867.0
25	23867.0
26	35229.0
27	46591.0
28	46591.0
29	46591.0
30	61074.5
31	75558.0
32	75558.0
33	75558.0
34	86279.0
35	97000.0
36	97000.0
37	97000.0
38	101944.5
39	106889.0
40	106889.0
41	106889.0
42	111061.5
43	115234.0
44	115234.0
45	115234.0
46	116711.5
47	118189.0
48	118189.0
49	118189.0
50	109904.5
51	101620.0
52	101620.0
53	101620.0
54	86964.5
55	72309.0
56	72309.0
57	72309.0
58	59973.0
59	47637.0
60	47637.0
61	47637.0
62	37076.0
63	26515.0
64	26515.0
65	26515.0
66	20228.0
67	13941.0
68	13941.0
69	13941.0
70	10168.5
71	6396.0
72	6396.0
73	6396.0
74	4546.0
75	2696.0
76	2696.0
77	2696.0
78	1918.5
79	1141.0
80	1141.0
81	1141.0
82	754.5
83	368.0
84	368.0
85	368.0
86	260.0
87	152.0
88	152.0
89	152.0
90	112.0
91	72.0
92	72.0
93	72.0
94	59.5
95	47.0
96	47.0
97	47.0
98	232.5
99	418.0
100	418.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040229720849167115
2	0.005828737963941827
3	0.002400068573387811
4	0.005600160004571559
5	0.01097174204977285
6	0.014971856338752535
7	0.02731506614474699
8	0.04034400982885225
9	0.05440155433012371
10	0.062287493928397955
11	0.058744535558158804
12	0.06777336495328437
13	0.06788765393296951
14	0.07371639189691134
15	0.06285893882682363
16	0.07280208005943027
17	0.06937341066887626
18	0.0806880196577045
19	0.07954512986085316
20	0.08045944169833423
21	0.07920226292179776
22	0.0789736849624275
23	0.08594531272322066
24	0.07954512986085316
25	0.08228806537329639
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	874975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.89461327934268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.89742369184657	22.51897904364625
2	17.46994243673704	13.58973310178529
3	8.677547048964094	10.12529510048279
4	4.961164677532183	7.718503261909962
5	3.024123953558574	5.881106584122879
6	2.020150331585254	4.7143779547886595
7	1.3981659547252234	3.8066786876566354
8	0.9931274194685612	3.0901845533872976
9	0.697778239405782	2.442583329878563
>10	2.697340831143178	16.306361150582298
>50	0.09032610750100646	2.5122743167522024
>100	0.06700571988024386	5.133793850061836
>500	0.004132511356617874	1.0232915416347337
>1k	0.0017710762956933746	1.1368375233105725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	2002	0.22880653732963802	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1937	0.2213777536501043	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1895	0.21657761650332868	No Hit
GTCCTACAGTGGACATTTCTAAATT	1825	0.20857738792536928	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1202	0.13737535358153088	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1041	0.11897482785222435	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1428897968513387E-4	0.0
3	0.0	0.0	0.0	1.1428897968513387E-4	0.0
4	0.0	0.0	0.0	1.1428897968513387E-4	0.0
5	0.0	0.0	0.0	1.1428897968513387E-4	0.0
6	0.0	0.0	0.0	1.1428897968513387E-4	0.0
7	0.0	0.0	0.0	2.2857795937026773E-4	0.0
8	0.0	0.0	0.0	2.2857795937026773E-4	0.0
9	0.0	0.0	0.0	5.714448984256693E-4	0.0
10	0.0	0.0	0.0	5.714448984256693E-4	0.0
11	0.0	0.0	0.0	5.714448984256693E-4	0.0
12	0.0	0.0	0.0	5.714448984256693E-4	0.0
13	0.0	0.0	0.0	6.857338781108031E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCTA	25	0.0060236147	19.003492	10
ACTTCCG	25	0.0060256063	19.002403	15
GCTCGCG	25	0.0060256063	19.002403	13
GGTCGTG	30	7.7154377E-4	19.001316	7
TAGCGAA	60	1.4622146E-6	15.836243	10
GCGAAAT	55	1.1252199E-5	15.54831	12
GTAGCGA	55	1.1252199E-5	15.54831	9
GGTATCA	300	0.0	14.880108	1
TAACGAT	40	0.0052842526	14.246911	4
GTATCAA	1120	0.0	14.077306	1
TCCAACG	75	9.651212E-7	13.9342985	18
TATTCCG	55	1.962213E-4	13.8151865	5
TAGGACC	320	0.0	13.35648	4
GTTCGTT	50	0.0014978491	13.300921	19
ACACCGC	50	0.0014991416	13.2994	6
TGTACCG	50	0.0015010821	13.297117	5
GGTAGCG	65	5.4407017E-5	13.15551	8
GATCTAT	60	4.0963283E-4	12.66392	1
CGTAAAT	85	3.957408E-6	12.28864	3
AGCGAAA	70	1.0879787E-4	12.21653	11
>>END_MODULE