FastQCFastQC Report
Thu 2 Feb 2017
SRR4064132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874975
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA10440.11931769479127975No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA9610.10983170947741364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC300.005745433729.33199913
ATCGGGC300.005745433729.33199912
ATCGGAT300.00574703929.33032217
CACCGTG554.9629525E-627.9971267
GTATCAA11600.026.9344271
GGTATCA5350.024.2647381
TATACGT856.1156807E-620.7037584
GGCGGTC550.0044793720.0025235
ATATCGC550.004483817619.99909215
TAGGACC4700.019.6575574
TAGGACG12700.018.8799514
GACCGTT708.126477E-418.8552077
TATCAAC16950.018.559632
TTAGGAC8950.018.4338633
CATGCGA600.00741245118.33249910
ACACGCC600.007414894618.3314518
GTCTTAC1102.791272E-618.0022681
AGGACGT13650.017.727125
TGTAGGA12400.017.5637192
GTAGGAC12650.017.5634163
Produced by FastQC (version 0.11.5)