##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874975
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3748827109346	32.0	32.0	32.0	32.0	32.0
2	31.4964073259236	32.0	32.0	32.0	32.0	32.0
3	31.537055344438414	32.0	32.0	32.0	32.0	32.0
4	31.59808794536987	32.0	32.0	32.0	32.0	32.0
5	31.578705677305067	32.0	32.0	32.0	32.0	32.0
6	35.16274064973285	36.0	36.0	36.0	36.0	36.0
7	35.17488613960399	36.0	36.0	36.0	36.0	36.0
8	35.128041372610646	36.0	36.0	36.0	36.0	36.0
9	35.22225549301409	36.0	36.0	36.0	36.0	36.0
10	35.117470784879565	36.0	36.0	36.0	36.0	36.0
11	35.21970113431812	36.0	36.0	36.0	36.0	36.0
12	35.15910283150947	36.0	36.0	36.0	36.0	36.0
13	35.181422326352184	36.0	36.0	36.0	36.0	36.0
14	35.14996085602446	36.0	36.0	36.0	36.0	36.0
15	35.12024114974714	36.0	36.0	36.0	36.0	36.0
16	35.141007457355926	36.0	36.0	36.0	36.0	36.0
17	35.13416954770136	36.0	36.0	36.0	36.0	36.0
18	35.12966999057116	36.0	36.0	36.0	36.0	36.0
19	35.10385096717049	36.0	36.0	36.0	36.0	36.0
20	35.10300180005143	36.0	36.0	36.0	36.0	36.0
21	35.09047801365753	36.0	36.0	36.0	36.0	36.0
22	35.084322409211694	36.0	36.0	36.0	36.0	36.0
23	35.05042315494728	36.0	36.0	36.0	36.0	36.0
24	35.02772079202263	36.0	36.0	36.0	36.0	36.0
25	35.002350924312125	36.0	36.0	36.0	36.0	36.0
26	34.963360096002745	36.0	36.0	36.0	36.0	36.0
27	34.93253978685105	36.0	36.0	36.0	36.0	36.0
28	34.92520129146547	36.0	36.0	36.0	36.0	36.0
29	34.90043944112689	36.0	36.0	36.0	36.0	36.0
30	34.866620189148264	36.0	36.0	36.0	36.0	36.0
31	34.86048058515958	36.0	36.0	36.0	36.0	36.0
32	34.84286179605132	36.0	36.0	36.0	32.0	36.0
33	34.83545929883711	36.0	36.0	36.0	32.0	36.0
34	34.82292751221463	36.0	36.0	36.0	32.0	36.0
35	34.79417126203606	36.0	36.0	36.0	32.0	36.0
36	34.76599102831509	36.0	36.0	36.0	32.0	36.0
37	34.75458498814252	36.0	36.0	36.0	32.0	36.0
38	34.71921254892997	36.0	36.0	36.0	32.0	36.0
39	34.6763701820052	36.0	36.0	36.0	32.0	36.0
40	34.69104831566616	36.0	36.0	36.0	32.0	36.0
41	34.671724334981	36.0	36.0	36.0	32.0	36.0
42	34.65607131632332	36.0	36.0	36.0	32.0	36.0
43	34.64255435869596	36.0	36.0	36.0	32.0	36.0
44	34.58184290979742	36.0	36.0	36.0	32.0	36.0
45	34.557522214920425	36.0	36.0	36.0	32.0	36.0
46	34.51673133518101	36.0	36.0	36.0	32.0	36.0
47	34.516134746707046	36.0	36.0	36.0	32.0	36.0
48	34.479764564701846	36.0	36.0	36.0	32.0	36.0
49	34.44067544786994	36.0	36.0	36.0	32.0	36.0
50	33.906293322666365	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	3.0
20	6.0
21	14.0
22	56.0
23	198.0
24	528.0
25	1217.0
26	2661.0
27	4929.0
28	8724.0
29	13502.0
30	19815.0
31	28437.0
32	40336.0
33	64345.0
34	133552.0
35	556651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.42086999458225	21.14956875170019	12.792404177839348	25.637157075878214
2	13.516333684202106	22.178792211399006	41.58080667816419	22.724067426234697
3	17.037459484417788	26.52839176567936	31.064194054209736	25.36995469569312
4	11.093002657218777	18.350467156204463	39.889025400725735	30.667504785851023
5	11.853138661104603	39.2212348924255	36.10434583845253	12.821280608017371
6	29.94438679180591	39.88527632884252	18.351672967820683	11.818663911530884
7	26.888913270565755	32.916863815381944	22.894508865406284	17.299714048646017
8	24.20857738792537	38.38703962970371	20.8441384039544	16.560244578416526
9	26.06941053844255	15.29941666438087	21.03928372237613	37.59188907480045
10	14.460268605643895	29.467874641556275	34.79964295633072	21.27221379646911
11	33.4768804558764	23.092914760895752	25.6215613263937	17.808643456834147
12	22.94479607394048	25.93939934215188	32.297302548418585	18.818502035489054
13	28.224835908097106	23.122814917591178	26.715636132562715	21.936713041749005
14	20.51331869669121	22.868248505953897	28.25252893517857	28.365903862176317
15	22.535462391178378	32.19062771089708	23.648337430752473	21.62557246717207
16	21.759906373611805	29.397565851054953	27.737846113053404	21.104681662279834
17	20.47681362324638	29.732963798965685	28.282865224720705	21.50735735306723
18	20.504656148996766	28.667545932898424	30.668067092432672	20.15973082567214
19	23.205718112766778	27.12183447736733	28.043004706425563	21.62944270344033
20	23.70478564432421	28.163333413335984	28.00344239980525	20.128438542534553
21	23.576102174347838	27.138146804194406	26.78807965941884	22.497671362038915
22	22.857795937026772	29.223463527529358	27.544786993914112	20.373953541529758
23	21.81364761602165	29.254736755485354	27.990001897216576	20.941613731276416
24	22.657436646471712	27.668802737462155	28.546660403601948	21.127100212464185
25	22.35942355629058	28.20512234399412	28.246838965839228	21.18861513387607
26	21.012820556780426	29.135249616251947	28.73567159624333	21.116258230724302
27	21.467816505792054	29.024636960584292	28.274498374109193	21.23304815951446
28	20.957623234587174	29.117639327174416	27.817660014378177	22.107077423860233
29	21.843162175321336	27.77041221018079	27.69680733122881	22.689618283269063
30	21.666558086427585	28.09050791549943	27.997128909888687	22.245805088184298
31	22.79121813613992	27.886235361083745	27.664837111695817	21.65770939108052
32	21.127626623692045	29.03381394046415	28.357992308000025	21.480567127843784
33	21.095841248288426	29.098460898407406	28.834095683520662	20.971602169783502
34	21.489278048401363	28.114806373966495	29.187458711171356	21.208456866460786
35	21.56680995317377	28.984829764885927	28.050819659015445	21.39754062292485
36	21.244762246570456	28.564929854680205	28.700717112166217	21.489590786583122
37	21.569039808899	28.249688546512292	27.491456459373893	22.68981518521482
38	21.679435905583453	29.14095388100662	27.755978186947143	21.423632026462787
39	22.191133878750886	27.509869406098343	28.03551386060078	22.263482854549984
40	22.94272547728769	27.61113854143808	28.348900043888527	21.097235937385708
41	22.891029223684797	27.111770965127935	28.93372238813817	21.063477423049097
42	21.597910749695696	28.629129498088474	28.770165322788028	21.002794429427798
43	20.66485021098011	28.843472417150878	28.898788978746552	21.592888393122458
44	20.453002530395604	28.013902318043527	28.95908622317312	22.574008928387748
45	20.959490904768654	28.22676132960899	28.49568667968054	22.31806108594182
46	21.677651918199782	27.6179166842865	28.22731527462367	22.47711612289005
47	22.109580993678506	27.72875281015737	28.509367953176806	21.65229824298732
48	20.92416181403615	28.14316164831334	28.18350658033842	22.74916995731209
49	21.69627162655463	26.97316238573842	28.830605989261297	22.499959998445654
50	20.867053806846688	28.56406499474835	28.036728410229916	22.53215278817504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	18.5
4	33.0
5	35.5
6	38.0
7	44.5
8	51.0
9	90.5
10	130.0
11	210.5
12	291.0
13	538.0
14	785.0
15	1084.0
16	1383.0
17	1770.5
18	2158.0
19	2476.0
20	2794.0
21	3480.5
22	4167.0
23	5260.5
24	6354.0
25	8599.0
26	10844.0
27	14586.5
28	18329.0
29	22269.5
30	26210.0
31	29958.0
32	33706.0
33	40264.5
34	46823.0
35	55209.0
36	63595.0
37	67661.5
38	71728.0
39	70529.0
40	69330.0
41	68452.5
42	67575.0
43	66111.0
44	64647.0
45	63651.5
46	62656.0
47	61150.5
48	59645.0
49	57192.5
50	54740.0
51	52435.0
52	50130.0
53	45979.0
54	41828.0
55	38053.0
56	34278.0
57	30198.5
58	26119.0
59	22728.5
60	19338.0
61	16338.0
62	13338.0
63	10949.5
64	8561.0
65	6941.5
66	5322.0
67	4231.5
68	3141.0
69	2457.5
70	1774.0
71	1465.5
72	1157.0
73	947.0
74	737.0
75	609.5
76	482.0
77	391.5
78	301.0
79	248.0
80	195.0
81	152.5
82	110.0
83	72.5
84	35.0
85	46.0
86	57.0
87	39.0
88	21.0
89	18.0
90	15.0
91	12.5
92	10.0
93	8.0
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008343095517014771
2	4.571559187405354E-4
3	3.428669390554016E-4
4	0.0
5	0.0
6	3.428669390554016E-4
7	1.1428897968513385E-4
8	0.0
9	8.000228577959371E-4
10	0.0013714677562216063
11	1.1428897968513385E-4
12	1.1428897968513385E-4
13	9.143118374810708E-4
14	2.285779593702677E-4
15	0.003885825309294551
16	9.143118374810708E-4
17	0.0
18	3.428669390554016E-4
19	1.1428897968513385E-4
20	4.571559187405354E-4
21	0.0
22	0.0
23	0.0010286008171662049
24	4.571559187405354E-4
25	0.0027429355124432126
26	0.005943026943626961
27	0.00800022857795937
28	0.004342981228035087
29	0.0037715363296094177
30	0.005257293065516157
31	0.009257407354495843
32	0.0045715591874053545
33	0.005600160004571559
34	0.004685848167090488
35	0.004342981228035087
36	0.008800251435755309
37	0.005143004085831024
38	0.009828852252921513
39	0.005600160004571559
40	0.00354295837023915
41	0.003885825309294551
42	0.0022857795937026772
43	0.0012571787765364723
44	0.0014857567359067402
45	0.0012571787765364723
46	0.0016000457155918742
47	0.0027429355124432126
48	0.0022857795937026772
49	0.0010286008171662049
50	0.0013714677562216063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	874975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.0677713911363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.01946047321463	28.40146637799587
2	15.822511160720476	14.26170631650106
3	7.6992136560167985	10.409592028226394
4	4.223442753940345	7.613646100725383
5	2.6381800507829563	5.944844770867131
6	1.750906522969508	4.7345672942663475
7	1.2024032686394304	3.7932744941697822
8	0.8221780289571354	2.9642985161484177
9	0.5948822645244308	2.4129016112005663
>10	2.1432593407460345	14.650105503204621
>50	0.04866084583651854	1.5090476576100926
>100	0.03362785140597298	2.8108531964150005
>500	0.001019025796618242	0.37383130417940447
>1k	2.547564491545605E-4	0.11986482848995647
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1044	0.11931769479127975	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	961	0.10983170947741364	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1428897968513387E-4	0.0
5	0.0	0.0	0.0	1.1428897968513387E-4	0.0
6	0.0	0.0	0.0	1.1428897968513387E-4	0.0
7	0.0	0.0	0.0	2.2857795937026773E-4	0.0
8	0.0	0.0	0.0	2.2857795937026773E-4	0.0
9	0.0	0.0	0.0	4.5715591874053547E-4	0.0
10	0.0	0.0	0.0	4.5715591874053547E-4	0.0
11	0.0	0.0	0.0	4.5715591874053547E-4	0.0
12	0.0	0.0	0.0	4.5715591874053547E-4	0.0
13	0.0	0.0	0.0	5.714448984256693E-4	0.0
14	0.0	0.0	0.0	5.714448984256693E-4	0.0
15	0.0	0.0	0.0	8.00022857795937E-4	0.0
16	0.0	0.0	0.0	8.00022857795937E-4	0.0
17	0.0	0.0	0.0	9.143118374810709E-4	0.0
18	0.0	0.0	0.0	9.143118374810709E-4	0.0
19	0.0	0.0	0.0	9.143118374810709E-4	0.0
20	0.0	0.0	0.0	0.0012571787765364726	0.0
21	0.0	0.0	0.0	0.0012571787765364726	0.0
22	0.0	0.0	0.0	0.0012571787765364726	0.0
23	0.0	0.0	0.0	0.0014857567359067402	0.0
24	0.0	0.0	0.0	0.0019429126546472756	0.0
25	0.0	0.0	0.0	0.0021714906140175433	0.0
26	0.0	0.0	0.0	0.0029715134718134805	0.0
27	0.0	0.0	0.0	0.003428669390554016	0.0
28	0.0	0.0	0.0	0.006057315923312095	0.0
29	0.0	0.0	0.0	0.012571787765364724	0.0
30	0.0	0.0	0.0	0.029486556758764534	0.0
31	0.0	0.0	0.0	0.07463070373439241	0.0
32	0.0	0.0	0.0	0.13531815194719848	0.0
33	0.0	0.0	0.0	0.19051972913511814	0.0
34	0.0	0.0	0.0	0.25395011286036745	0.0
35	0.0	0.0	0.0	0.3274379267979085	0.0
36	0.0	0.0	0.0	0.43326952198634244	0.0
37	0.0	0.0	0.0	0.5973884968141947	0.0
38	0.0	0.0	0.0	0.8220806308751679	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGCC	30	0.0057454337	29.331999	13
ATCGGGC	30	0.0057454337	29.331999	12
ATCGGAT	30	0.005747039	29.330322	17
CACCGTG	55	4.9629525E-6	27.997126	7
GTATCAA	1160	0.0	26.934427	1
GGTATCA	535	0.0	24.264738	1
TATACGT	85	6.1156807E-6	20.703758	4
GGCGGTC	55	0.00447937	20.00252	35
ATATCGC	55	0.0044838176	19.999092	15
TAGGACC	470	0.0	19.657557	4
TAGGACG	1270	0.0	18.879951	4
GACCGTT	70	8.126477E-4	18.855207	7
TATCAAC	1695	0.0	18.55963	2
TTAGGAC	895	0.0	18.433863	3
CATGCGA	60	0.007412451	18.332499	10
ACACGCC	60	0.0074148946	18.331451	8
GTCTTAC	110	2.791272E-6	18.002268	1
AGGACGT	1365	0.0	17.72712	5
TGTAGGA	1240	0.0	17.563719	2
GTAGGAC	1265	0.0	17.563416	3
>>END_MODULE