FastQCFastQC Report
Thu 2 Feb 2017
SRR4064131_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064131_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146123
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22690.19797177091813006No Hit
TATCAACGCAGAGTACTTTTTTTTT15600.13611104567310836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGT508.7109176E-515.19996556
TCGCGTA655.445698E-513.1549649
CGCGGTC655.44793E-513.1543910
AAGACGG2300.012.80325
TAGTACT751.4791411E-512.6655314
TTGGAAC604.101205E-412.6627663
CAAGACG2300.012.3901944
CGCATCG1001.4403486E-712.35051213
CGACCAT2000.012.35051210
AACCGCG701.0895515E-412.2153247
ATTAAAC1405.2750693E-1112.21052553
GTAGTAC550.003074680712.0871873
GGTATCA6250.011.8544211
GAACCGC1052.7173337E-711.7618776
CCGACCA1950.011.6933039
CGGAATT658.014874E-411.69330215
ACCGTGT658.014874E-411.6933028
GCATAGG1157.0782335E-811.5636761
GCGTAAC752.0712696E-411.400969511
CGCGTAA752.07211E-411.40047210
Produced by FastQC (version 0.11.5)