##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064131_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1146123
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22083231904429	32.0	32.0	32.0	32.0	32.0
2	30.749999781873324	32.0	32.0	32.0	32.0	32.0
3	30.770276837651807	32.0	32.0	32.0	32.0	32.0
4	30.80716991108284	32.0	32.0	32.0	32.0	32.0
5	30.68988494254107	32.0	32.0	32.0	32.0	32.0
6	34.35072151941807	36.0	36.0	36.0	32.0	36.0
7	34.28659751178539	36.0	36.0	36.0	32.0	36.0
8	34.245675202399745	36.0	36.0	36.0	32.0	36.0
9	34.41820031532392	36.0	36.0	36.0	32.0	36.0
10	34.059491869546285	36.0	36.0	36.0	32.0	36.0
11	34.3887907318848	36.0	36.0	36.0	32.0	36.0
12	34.17734832997854	36.0	36.0	36.0	32.0	36.0
13	34.28073950178122	36.0	36.0	36.0	32.0	36.0
14	34.1649927625569	36.0	36.0	36.0	32.0	36.0
15	34.08254349664041	36.0	36.0	36.0	32.0	36.0
16	34.090594988496	36.0	36.0	36.0	32.0	36.0
17	34.00797994630594	36.0	36.0	36.0	32.0	36.0
18	33.99782484078934	36.0	36.0	36.0	32.0	36.0
19	34.02466401948133	36.0	36.0	36.0	32.0	36.0
20	34.005672166076415	36.0	36.0	36.0	32.0	36.0
21	33.98573189788531	36.0	36.0	36.0	32.0	36.0
22	33.95925568198178	36.0	36.0	36.0	32.0	36.0
23	33.90497791249281	36.0	36.0	36.0	32.0	36.0
24	33.892747986036404	36.0	36.0	36.0	32.0	36.0
25	33.46440827031654	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	9.0
5	14.0
6	53.0
7	20.0
8	65.0
9	72.0
10	101.0
11	29.0
12	73.0
13	66.0
14	103.0
15	203.0
16	314.0
17	421.0
18	578.0
19	816.0
20	1263.0
21	1926.0
22	2963.0
23	4641.0
24	6922.0
25	9901.0
26	14122.0
27	18397.0
28	24971.0
29	33185.0
30	43114.0
31	58288.0
32	81666.0
33	114937.0
34	241119.0
35	485767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.827045059970274	16.85347457471778	11.065586291113087	26.253894074198865
2	17.0456320311607	19.63171180541316	36.98771180192294	26.334944361503204
3	19.061949219090828	23.571067097111946	28.284181136026525	29.082802547770704
4	12.955347599415395	15.293327225530614	35.368278690312586	36.3830464847414
5	14.933639322519001	36.337466514253805	33.36532840027574	15.36356576295146
6	34.569413793314084	34.8896690201499	16.815493720990503	13.72542346554551
7	30.628341930973935	30.04388854804744	20.491387668792875	18.836381852185742
8	28.45200189925566	32.598070021087324	19.233280265895793	19.716647813761224
9	27.801657163408322	13.995541092135632	18.023659796154234	40.17914194830181
10	16.20119779298785	26.391430367369743	31.30552451459701	26.101847325045398
11	37.47399573106588	20.75059909121464	22.347040773122302	19.42836440459718
12	25.1631986855052	23.23284001945201	28.46934169618372	23.13461959885907
13	30.10285732246177	19.193398133184562	24.76477095234478	25.93897359200889
14	23.979401623807224	19.268457295825755	24.810862866956597	31.941278213410424
15	25.539389653531693	26.781210303689058	21.856183130131875	25.82321691264738
16	26.01072695507618	25.28412985151121	23.39333594682448	25.31180724658813
17	24.516125089380115	25.384214445921867	24.80083185712452	25.298828607573498
18	25.24460557923387	24.600706769320237	25.671077207606285	24.483610443839606
19	26.1115607910525	24.427836847385613	24.864855897224995	24.595746464336887
20	26.271223441227527	23.98724759779112	24.44935190133392	25.29217705964743
21	27.12030309565867	23.843985019851576	23.903186119350465	25.132525765139285
22	26.37406873137899	23.960541803613655	24.29445377799045	25.3709356870169
23	24.965528804297048	23.83695189925591	24.964044294285774	26.23347500216127
24	25.311596797149917	24.358425818394558	24.813880181273632	25.516097203181893
25	25.376062786134728	24.10381926283224	24.748760282949753	25.77135766808328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	84.0
2	203.0
3	322.0
4	322.0
5	322.0
6	798.0
7	1274.0
8	1274.0
9	1274.0
10	1438.0
11	1602.0
12	1602.0
13	1602.0
14	1991.0
15	2380.0
16	2380.0
17	2380.0
18	4103.0
19	5826.0
20	5826.0
21	5826.0
22	9632.0
23	13438.0
24	13438.0
25	13438.0
26	20653.5
27	27869.0
28	27869.0
29	27869.0
30	35866.5
31	43864.0
32	43864.0
33	43864.0
34	55483.0
35	67102.0
36	67102.0
37	67102.0
38	78434.5
39	89767.0
40	89767.0
41	89767.0
42	104264.0
43	118761.0
44	118761.0
45	118761.0
46	133270.5
47	147780.0
48	147780.0
49	147780.0
50	152657.5
51	157535.0
52	157535.0
53	157535.0
54	149108.5
55	140682.0
56	140682.0
57	140682.0
58	129897.5
59	119113.0
60	119113.0
61	119113.0
62	104112.5
63	89112.0
64	89112.0
65	89112.0
66	73311.5
67	57511.0
68	57511.0
69	57511.0
70	43615.0
71	29719.0
72	29719.0
73	29719.0
74	22806.5
75	15894.0
76	15894.0
77	15894.0
78	12538.5
79	9183.0
80	9183.0
81	9183.0
82	6443.5
83	3704.0
84	3704.0
85	3704.0
86	2781.5
87	1859.0
88	1859.0
89	1859.0
90	1343.5
91	828.0
92	828.0
93	828.0
94	559.0
95	290.0
96	290.0
97	290.0
98	457.0
99	624.0
100	624.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04118231638314561
2	0.005584042899409575
3	0.002006765416975316
4	0.004188032174557181
5	0.00985932574427003
6	0.014134608589130486
7	0.023208678300671044
8	0.03586002549464586
9	0.0480751193371043
10	0.059592207817136554
11	0.055665927653489204
12	0.06456549602442321
13	0.06543800272745595
14	0.06892802953958693
15	0.061773474574718414
16	0.0684045255177673
17	0.06404199200260356
18	0.07957261131658644
19	0.07538457914202927
20	0.08088137137113556
21	0.076257085845062
22	0.07948536064628317
23	0.08410964617235672
24	0.07791484858082422
25	0.0787001046135537
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1146123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.35059952051791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15811182197193	48.16782592587266
2	11.41100784723469	13.545003137334263
3	3.1480047524454413	5.605079080532295
4	1.3412026205610108	3.1840471843514275
5	0.7464762331127038	2.2151905981528426
6	0.46838577820042204	1.6679386045847626
7	0.31235220347096787	1.2976803376289716
8	0.22496117863140302	1.0681264656492875
9	0.17245121000525904	0.9211574431665774
>10	0.8819628609223412	9.243729491412507
>50	0.06652906385453977	2.799678906929688
>100	0.062053581962642886	7.4258991763124405
>500	0.0062053545527288485	2.5228845521070147
>1k	2.95493073939469E-4	0.33575909596530396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2269	0.19797177091813006	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1560	0.13611104567310836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.72506703032746E-5
2	0.0	0.0	0.0	0.0	8.72506703032746E-5
3	0.0	0.0	0.0	0.0	8.72506703032746E-5
4	0.0	0.0	0.0	0.0	8.72506703032746E-5
5	0.0	0.0	0.0	0.0	8.72506703032746E-5
6	0.0	0.0	0.0	8.72506703032746E-5	8.72506703032746E-5
7	0.0	0.0	0.0	8.72506703032746E-5	8.72506703032746E-5
8	0.0	0.0	0.0	8.72506703032746E-5	8.72506703032746E-5
9	0.0	0.0	0.0	8.72506703032746E-5	8.72506703032746E-5
10	0.0	0.0	0.0	8.72506703032746E-5	8.72506703032746E-5
11	0.0	0.0	0.0	8.72506703032746E-5	8.72506703032746E-5
12	0.0	0.0	0.0	8.72506703032746E-5	6.107546921229223E-4
13	0.0	0.0	0.0	8.72506703032746E-5	6.107546921229223E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCCGT	50	8.7109176E-5	15.1999655	6
TCGCGTA	65	5.445698E-5	13.154964	9
CGCGGTC	65	5.44793E-5	13.15439	10
AAGACGG	230	0.0	12.8032	5
TAGTACT	75	1.4791411E-5	12.665531	4
TTGGAAC	60	4.101205E-4	12.662766	3
CAAGACG	230	0.0	12.390194	4
CGCATCG	100	1.4403486E-7	12.350512	13
CGACCAT	200	0.0	12.350512	10
AACCGCG	70	1.0895515E-4	12.215324	7
ATTAAAC	140	5.2750693E-11	12.2105255	3
GTAGTAC	55	0.0030746807	12.087187	3
GGTATCA	625	0.0	11.854421	1
GAACCGC	105	2.7173337E-7	11.761877	6
CCGACCA	195	0.0	11.693303	9
CGGAATT	65	8.014874E-4	11.693302	15
ACCGTGT	65	8.014874E-4	11.693302	8
GCATAGG	115	7.0782335E-8	11.563676	1
GCGTAAC	75	2.0712696E-4	11.4009695	11
CGCGTAA	75	2.07211E-4	11.400472	10
>>END_MODULE