FastQCFastQC Report
Thu 2 Feb 2017
SRR4064131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146123
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC752.0608222E-623.47097436
GTATCAA16400.022.9382271
GGTATCA7850.020.1776431
ACCGTAT550.004486636719.997568
ATAGACC1101.2677083E-719.9966893
TTAACGG808.975399E-519.2535335
AGGATCG808.999491E-519.246815
GTATAGT1303.5881385E-818.6147381
AATCCCG600.00741708918.33109719
GTTCGCG600.007418955718.33029716
GTAGGAC3300.017.9970193
TTAGGAC1852.1827873E-1117.8348853
GTACTAT1002.4835716E-517.5993881
TATTAGG1002.4863668E-517.5970862
AACGCAG21900.017.3761186
TATATGG1154.27924E-617.214542
TCCTATA1154.27924E-617.214542
CTAGTAC1154.27924E-617.214543
GTTTAAT1954.9112714E-1116.922491
TAGGACG1702.4720066E-916.8207444
Produced by FastQC (version 0.11.5)