##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1146123
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331857924498504	32.0	32.0	32.0	32.0	32.0
2	31.400482321705436	32.0	32.0	32.0	32.0	32.0
3	31.477811718288525	32.0	32.0	32.0	32.0	32.0
4	31.55875940016909	32.0	32.0	32.0	32.0	32.0
5	31.510972208043988	32.0	32.0	32.0	32.0	32.0
6	35.09879044395758	36.0	36.0	36.0	36.0	36.0
7	35.11415528699799	36.0	36.0	36.0	36.0	36.0
8	35.05956341509594	36.0	36.0	36.0	36.0	36.0
9	35.16571607061371	36.0	36.0	36.0	36.0	36.0
10	35.02544316796714	36.0	36.0	36.0	36.0	36.0
11	35.16693932501136	36.0	36.0	36.0	36.0	36.0
12	35.07482791986549	36.0	36.0	36.0	36.0	36.0
13	35.11359950022816	36.0	36.0	36.0	36.0	36.0
14	35.05246731808017	36.0	36.0	36.0	36.0	36.0
15	35.022742759721254	36.0	36.0	36.0	36.0	36.0
16	35.04642259164156	36.0	36.0	36.0	36.0	36.0
17	35.007696381627454	36.0	36.0	36.0	36.0	36.0
18	35.004374748609	36.0	36.0	36.0	36.0	36.0
19	34.997246368845225	36.0	36.0	36.0	36.0	36.0
20	34.98949763681559	36.0	36.0	36.0	36.0	36.0
21	34.99082908204442	36.0	36.0	36.0	36.0	36.0
22	34.97593364761025	36.0	36.0	36.0	36.0	36.0
23	34.92543993969234	36.0	36.0	36.0	32.0	36.0
24	34.89236757311388	36.0	36.0	36.0	32.0	36.0
25	34.86459917478316	36.0	36.0	36.0	32.0	36.0
26	34.80128310835748	36.0	36.0	36.0	32.0	36.0
27	34.80248280507415	36.0	36.0	36.0	32.0	36.0
28	34.77437151160914	36.0	36.0	36.0	32.0	36.0
29	34.74839087951293	36.0	36.0	36.0	32.0	36.0
30	34.724214591278596	36.0	36.0	36.0	32.0	36.0
31	34.723810620675096	36.0	36.0	36.0	32.0	36.0
32	34.68838423101185	36.0	36.0	36.0	32.0	36.0
33	34.6648361476037	36.0	36.0	36.0	32.0	36.0
34	34.65715547109691	36.0	36.0	36.0	32.0	36.0
35	34.61329106910864	36.0	36.0	36.0	32.0	36.0
36	34.600568176365016	36.0	36.0	36.0	32.0	36.0
37	34.585981609303715	36.0	36.0	36.0	32.0	36.0
38	34.54423652609711	36.0	36.0	36.0	32.0	36.0
39	34.55074629860844	36.0	36.0	36.0	32.0	36.0
40	34.52182619142972	36.0	36.0	36.0	32.0	36.0
41	34.5147728472424	36.0	36.0	36.0	32.0	36.0
42	34.44693806860171	36.0	36.0	36.0	32.0	36.0
43	34.444626798345375	36.0	36.0	36.0	32.0	36.0
44	34.38637039829059	36.0	36.0	36.0	32.0	36.0
45	34.35584487877828	36.0	36.0	36.0	32.0	36.0
46	34.33387777751602	36.0	36.0	36.0	32.0	36.0
47	34.305530034734495	36.0	36.0	36.0	32.0	36.0
48	34.26200067532019	36.0	36.0	36.0	32.0	36.0
49	34.24585842880738	36.0	36.0	36.0	32.0	36.0
50	33.64259333422329	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	13.0
21	37.0
22	137.0
23	410.0
24	1067.0
25	2280.0
26	4520.0
27	8119.0
28	13233.0
29	20148.0
30	28693.0
31	40498.0
32	57940.0
33	93317.0
34	195414.0
35	680290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83619975044283	17.714108705705783	11.636257340558274	26.813434203293106
2	16.222705785247992	20.357082404839975	36.57603640799147	26.844175401920563
3	18.304889540362264	24.072784699682405	28.593515513209788	29.02881024674554
4	12.650125684590572	15.886863800831149	35.416704838834924	36.046305675743355
5	14.551492291839532	36.570682204266035	33.272694117472554	15.605131386421874
6	34.64133835664964	35.232025909981665	16.55661439183612	13.570021341532575
7	30.499458172864667	30.20629566485941	20.595625945579965	18.698620216695954
8	28.297050142087716	32.838622032713765	19.13564250957358	19.72868531562494
9	27.83058913039116	13.814257407428412	17.955297601545396	40.39985586063503
10	16.078721868998727	26.539837764405018	31.376891069015127	26.004549297581125
11	37.69167881632251	20.624400696958354	22.400475341651813	19.283445145067326
12	25.171491342108432	23.225188941491396	28.53151758713296	23.071802129267216
13	30.05661725635341	19.14516580767932	24.84083213144906	25.957384804518213
14	23.966076850591563	19.186821624262727	24.777510208355146	32.069591316790564
15	25.492488659040003	26.878533279467952	21.852910868212767	25.776067193279278
16	26.084923192899858	25.26554857353257	23.361468888892766	25.288059344674807
17	24.56350670914029	25.537136939054534	24.767411525639048	25.131944826166126
18	25.273052163913313	24.564050123983744	25.632177489577863	24.53072022252508
19	26.19736276123941	24.336305963670565	24.98414219067238	24.48218908441764
20	26.460287406967918	23.843871879662508	24.489097905087647	25.20674280828193
21	27.22918918824594	23.75259897934166	23.959470318630725	25.05874151378168
22	26.455735078813596	23.862206641177817	24.342085746543564	25.339972533465023
23	24.970792634247	23.81558569602527	24.993652469429335	26.219969200298397
24	25.449561039962727	24.20946185296802	24.799627961518798	25.541349145550456
25	25.427229244066428	23.91881728217111	24.85014475100907	25.80380872275339
26	25.248437476822826	24.73642373935813	25.03789058716667	24.977248196652365
27	25.693149523095677	24.355321861336066	24.436645588231446	25.514883027336815
28	25.12505682418053	24.27712225771522	25.036930263969587	25.56089065413466
29	25.203757816787668	24.359845771409077	24.915473553147176	25.520922858656075
30	25.082563540115192	24.521611361092134	24.979517261778554	25.416307837014116
31	25.723255787573308	24.200001221605312	24.25287928009054	25.823863710730844
32	25.488904381270817	24.44046373604704	24.15575486243143	25.914877020250714
33	25.126454653889006	24.1858431866311	24.709723636484206	25.97797852299568
34	25.651350691051235	24.152327935222672	24.871649448555075	25.324671925171018
35	26.065560467922595	24.189350372179426	24.822722882294276	24.922366277603707
36	25.226477491863214	24.550578955175695	24.564889226285523	25.65805432667557
37	25.942601065890457	24.341322276552155	24.340886008355408	25.37519064920198
38	25.344715353511027	24.389669272210384	24.726225940604774	25.539389433673815
39	25.80114879244167	24.16844302239037	24.407345779893603	25.62306240527436
40	26.045966601314202	24.254314440451168	24.70000253033567	24.999716427898957
41	25.224263079504432	24.521698614503087	25.00150512133896	25.25253318465352
42	26.151420549233666	24.676600593662364	24.506721018823736	24.66525783828023
43	25.472492437416182	23.976902599504584	24.7823275918482	25.76827737123103
44	25.26537592377695	24.424444425055185	24.452888466203067	25.857291184964794
45	25.346542082821095	24.60455331909967	24.54208107605821	25.506823522021026
46	25.363816656821797	24.252495172814548	24.722344131861345	25.66134403850231
47	25.53878370124771	24.222929936305732	24.86528226158276	25.3730041008638
48	25.993520640851024	24.70977688702828	24.108259219737178	25.188443252383514
49	25.29900882979095	24.80569224863016	24.23891041077723	25.65638851080166
50	25.371408827607144	24.98470913324998	24.282768916394517	25.361113122748357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	17.5
2	20.0
3	57.5
4	95.0
5	120.0
6	145.0
7	143.0
8	141.0
9	194.0
10	247.0
11	339.0
12	431.0
13	845.0
14	1259.0
15	1712.5
16	2166.0
17	2242.0
18	2318.0
19	2313.5
20	2309.0
21	2498.5
22	2688.0
23	3142.0
24	3596.0
25	4377.5
26	5159.0
27	6573.5
28	7988.0
29	9581.5
30	11175.0
31	13115.5
32	15056.0
33	17871.0
34	20686.0
35	24697.5
36	28709.0
37	33711.0
38	38713.0
39	42560.5
40	46408.0
41	50880.5
42	55353.0
43	58003.0
44	60653.0
45	66986.0
46	73319.0
47	78436.0
48	83553.0
49	86981.0
50	90409.0
51	89125.5
52	87842.0
53	87421.5
54	87001.0
55	87188.5
56	87376.0
57	85120.5
58	82865.0
59	76021.5
60	69178.0
61	60911.5
62	52645.0
63	45627.5
64	38610.0
65	32823.5
66	27037.0
67	23434.5
68	19832.0
69	17664.5
70	15497.0
71	12188.5
72	8880.0
73	7585.5
74	6291.0
75	5021.5
76	3752.0
77	3251.0
78	2750.0
79	2224.5
80	1699.0
81	1364.0
82	1029.0
83	827.5
84	626.0
85	456.0
86	286.0
87	213.0
88	140.0
89	108.0
90	76.0
91	53.5
92	31.0
93	31.5
94	32.0
95	27.5
96	23.0
97	14.5
98	6.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008114312338204538
2	8.72506703032746E-5
3	2.617520109098238E-4
4	0.0
5	0.0
6	8.72506703032746E-5
7	8.72506703032746E-5
8	0.0
9	8.725067030327461E-4
10	0.0010470080436392952
11	0.0
12	8.72506703032746E-5
13	5.235040218196476E-4
14	2.617520109098238E-4
15	0.004886037536983378
16	4.3625335151637303E-4
17	0.0
18	4.3625335151637303E-4
19	0.0
20	0.00113425871394257
21	0.0
22	8.72506703032746E-5
23	6.980053624261968E-4
24	4.3625335151637303E-4
25	0.0014832613951556682
26	0.005235040218196476
27	0.007503557646081616
28	0.0038390294933440825
29	0.0027920214497047873
30	0.0031410241309178855
31	0.007852560327294714
32	0.0034900268121309842
33	0.005235040218196476
34	0.003751778823040808
35	0.0031410241309178855
36	0.008114312338204538
37	0.0034027761418277095
38	0.009161320381843833
39	0.00436253351516373
40	0.002617520109098238
41	0.0031410241309178855
42	0.0014832613951556682
43	0.0017450134060654921
44	0.00113425871394257
45	0.0012215093842458445
46	8.725067030327461E-4
47	0.002006765416975316
48	0.0019195147466720412
49	2.617520109098238E-4
50	0.0012215093842458445
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1146123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.48581640636567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.00573455818902	51.24195273872155
2	11.206305925923782	14.004703493616821
3	2.9526767985776456	5.535012610297748
4	1.2424994603935156	3.105543726686305
5	0.6611744268434971	2.0657011924163404
6	0.4115606397454061	1.543002154513071
7	0.28967406235988535	1.2670364194814143
8	0.19907581947150735	0.9951532085154723
9	0.14888241174193903	0.8372735141619368
>10	0.7693952974588703	8.45042848448899
>50	0.05435482637561118	2.3383859999168775
>100	0.05402316203182607	6.645606034422171
>500	0.0046426108875949985	1.97020042276128
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.72506703032746E-5
2	0.0	0.0	0.0	0.0	8.72506703032746E-5
3	0.0	0.0	0.0	0.0	8.72506703032746E-5
4	0.0	0.0	0.0	0.0	8.72506703032746E-5
5	0.0	0.0	0.0	0.0	8.72506703032746E-5
6	0.0	0.0	0.0	0.0	8.72506703032746E-5
7	0.0	0.0	0.0	0.0	8.72506703032746E-5
8	0.0	0.0	0.0	0.0	8.72506703032746E-5
9	0.0	0.0	0.0	0.0	8.72506703032746E-5
10	0.0	0.0	0.0	0.0	8.72506703032746E-5
11	0.0	0.0	0.0	0.0	8.72506703032746E-5
12	0.0	0.0	0.0	0.0	8.72506703032746E-5
13	0.0	0.0	0.0	0.0	8.72506703032746E-5
14	0.0	0.0	0.0	0.0	1.745013406065492E-4
15	0.0	0.0	0.0	0.0	3.490026812130984E-4
16	0.0	0.0	0.0	0.0	4.3625335151637303E-4
17	0.0	0.0	0.0	0.0	4.3625335151637303E-4
18	0.0	0.0	0.0	0.0	4.3625335151637303E-4
19	0.0	0.0	0.0	8.72506703032746E-5	4.3625335151637303E-4
20	0.0	0.0	0.0	8.72506703032746E-5	6.980053624261968E-4
21	0.0	0.0	0.0	8.72506703032746E-5	6.980053624261968E-4
22	0.0	0.0	0.0	2.617520109098238E-4	6.980053624261968E-4
23	0.0	0.0	0.0	6.107546921229223E-4	6.980053624261968E-4
24	0.0	0.0	0.0	0.0010470080436392952	6.980053624261968E-4
25	0.0	0.0	0.0	0.0010470080436392952	6.980053624261968E-4
26	0.0	0.0	0.0	0.001308760054549119	7.852560327294715E-4
27	0.0	0.0	0.0	0.001570512065458943	7.852560327294715E-4
28	0.0	0.0	0.0	0.002704770779401513	7.852560327294715E-4
29	0.0	0.0	0.0	0.005409541558803026	7.852560327294715E-4
30	0.0	0.0	0.0	0.013523853897007564	7.852560327294715E-4
31	0.0	0.0	0.0	0.0326317506934247	7.852560327294715E-4
32	0.0	0.0	0.0	0.05313565821469424	7.852560327294715E-4
33	0.0	0.0	0.0	0.07137104830807862	7.852560327294715E-4
34	0.0	0.0	0.0	0.093096465213594	7.852560327294715E-4
35	0.0	0.0	0.0	0.11979517032639603	7.852560327294715E-4
36	0.0	0.0	0.0	0.15740020922710737	7.852560327294715E-4
37	0.0	0.0	0.0	0.21289163553999005	7.852560327294715E-4
38	0.0	0.0	0.0	0.2858331959135276	7.852560327294715E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGC	75	2.0608222E-6	23.470974	36
GTATCAA	1640	0.0	22.938227	1
GGTATCA	785	0.0	20.177643	1
ACCGTAT	55	0.0044866367	19.99756	8
ATAGACC	110	1.2677083E-7	19.996689	3
TTAACGG	80	8.975399E-5	19.25353	35
AGGATCG	80	8.999491E-5	19.24681	5
GTATAGT	130	3.5881385E-8	18.614738	1
AATCCCG	60	0.007417089	18.331097	19
GTTCGCG	60	0.0074189557	18.330297	16
GTAGGAC	330	0.0	17.997019	3
TTAGGAC	185	2.1827873E-11	17.834885	3
GTACTAT	100	2.4835716E-5	17.599388	1
TATTAGG	100	2.4863668E-5	17.597086	2
AACGCAG	2190	0.0	17.376118	6
TATATGG	115	4.27924E-6	17.21454	2
TCCTATA	115	4.27924E-6	17.21454	2
CTAGTAC	115	4.27924E-6	17.21454	3
GTTTAAT	195	4.9112714E-11	16.92249	1
TAGGACG	170	2.4720066E-9	16.820744	4
>>END_MODULE