FastQCFastQC Report
Thu 2 Feb 2017
SRR4064130_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064130_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935512
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21310.2277897023234336No Hit
TATCAACGCAGAGTACTTTTTTTTT15390.16450884649261582No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12580.1344718186404878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTC250.006027966319.00128414
TCTTATA1400.014.2456282
GCGTTAT551.9621603E-413.8154211
ACCTTAT551.9621603E-413.8154211
CGTTATT707.278173E-613.5672652
AATCGAA500.001498004913.3008997
TAAGGCC500.001499213213.2994764
GTTTTCG655.444503E-513.15473415
GTCCTAT1700.012.8503721
TAAGACT751.4776901E-512.6661674
GTATTAC604.0962166E-412.6641361
GTTATAA604.0962166E-412.6641361
TGTACCG853.940302E-612.2936335
CTAGGAC853.949739E-612.2910033
CGCGGTC550.003064091812.09237310
CAGTCCG550.003064091812.0923739
CTATGTT550.003067777312.0904344
AAGAATT1459.640644E-1111.79326916
TCCTATG658.038844E-411.688722
CGCCGGT1552.5465852E-1111.6459477
Produced by FastQC (version 0.11.5)