##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064130_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935512
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23469394299592	32.0	32.0	32.0	32.0	32.0
2	30.774322510026597	32.0	32.0	32.0	32.0	32.0
3	30.804482465216907	32.0	32.0	32.0	32.0	32.0
4	30.838913878175802	32.0	32.0	32.0	32.0	32.0
5	30.720191723890235	32.0	32.0	32.0	32.0	32.0
6	34.395778995886744	36.0	36.0	36.0	32.0	36.0
7	34.32540256030922	36.0	36.0	36.0	32.0	36.0
8	34.2740296222817	36.0	36.0	36.0	32.0	36.0
9	34.44652660788958	36.0	36.0	36.0	32.0	36.0
10	34.083412078091996	36.0	36.0	36.0	32.0	36.0
11	34.43271064401098	36.0	36.0	36.0	32.0	36.0
12	34.20906733425119	36.0	36.0	36.0	32.0	36.0
13	34.31132577668699	36.0	36.0	36.0	32.0	36.0
14	34.199464036805516	36.0	36.0	36.0	32.0	36.0
15	34.12904591282634	36.0	36.0	36.0	32.0	36.0
16	34.11882370295624	36.0	36.0	36.0	32.0	36.0
17	34.049399687016304	36.0	36.0	36.0	32.0	36.0
18	34.03964673889806	36.0	36.0	36.0	32.0	36.0
19	34.067366319192054	36.0	36.0	36.0	32.0	36.0
20	34.04471241416465	36.0	36.0	36.0	32.0	36.0
21	34.03463451030024	36.0	36.0	36.0	32.0	36.0
22	34.012984333712446	36.0	36.0	36.0	32.0	36.0
23	33.952304192784275	36.0	36.0	36.0	32.0	36.0
24	33.93102600501116	36.0	36.0	36.0	32.0	36.0
25	33.525652263145744	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	6.0
5	13.0
6	33.0
7	15.0
8	51.0
9	62.0
10	86.0
11	35.0
12	59.0
13	47.0
14	90.0
15	143.0
16	272.0
17	324.0
18	437.0
19	594.0
20	932.0
21	1388.0
22	2282.0
23	3416.0
24	5142.0
25	7552.0
26	10894.0
27	14523.0
28	19587.0
29	26681.0
30	34843.0
31	47921.0
32	67333.0
33	95144.0
34	198693.0
35	396910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79565674884938	16.875224562424076	11.108461512139204	26.220657176587338
2	16.798989167977062	19.769270321668213	37.32413863877614	26.107601871578577
3	18.935623455364855	23.823511805502108	28.488737525334155	28.752127213798882
4	12.736565070894693	15.298052747703833	35.75831238134332	36.20706980005815
5	14.597883128420191	36.576167074853785	33.81339416764571	15.012555629080312
6	34.3011210201433	35.200524268813915	16.912373128871348	13.585981582171438
7	30.244219538846114	30.259829403425186	21.002324372985953	18.493626684742747
8	28.207785041992008	32.90817363111621	19.492545804114297	19.391495522777483
9	27.720472403050533	13.999052456074496	18.300859099362707	39.97961604151226
10	15.794697807545774	26.823604660863758	32.07937672605767	25.302320805532798
11	37.11979499224055	20.85810635205334	22.757380033347985	19.264718622358124
12	24.97825963715789	23.399369128363873	29.069298543048056	22.553072691430177
13	29.808804103090687	19.51075242939122	25.024681652146473	25.655761815371612
14	23.5888966497627	19.384012433561633	25.446764268271423	31.58032664840425
15	25.225765806815275	27.102164977233567	22.213558462728187	25.458510753222974
16	25.668647686528207	25.484344380835022	23.87385652795249	24.973151404684277
17	24.168596473400754	25.70031928719268	25.286262093604094	24.84482214580247
18	24.760100388760396	24.99331383469232	25.96810324722979	24.278482529317497
19	25.69800130935418	24.681005378621585	25.33675220258191	24.284241109442327
20	26.04475568164799	24.24451519313966	24.89783517550954	24.812893949702815
21	26.54110266814582	24.12981175028269	24.522957008629938	24.80612857294155
22	25.885698208438217	24.431331310021385	24.73236760661167	24.950602874928727
23	24.691301231371625	24.279096637531694	25.266654541953294	25.762947589143387
24	24.982749601234968	24.656676947369434	25.26463983841859	25.095933612977007
25	25.011366371586746	24.395817175256077	25.34898772431869	25.24382872883849
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	255.0
1	255.0
2	331.0
3	407.0
4	407.0
5	407.0
6	1119.5
7	1832.0
8	1832.0
9	1832.0
10	1856.0
11	1880.0
12	1880.0
13	1880.0
14	2120.0
15	2360.0
16	2360.0
17	2360.0
18	4087.0
19	5814.0
20	5814.0
21	5814.0
22	9263.5
23	12713.0
24	12713.0
25	12713.0
26	19049.5
27	25386.0
28	25386.0
29	25386.0
30	31814.5
31	38243.0
32	38243.0
33	38243.0
34	47049.0
35	55855.0
36	55855.0
37	55855.0
38	65441.0
39	75027.0
40	75027.0
41	75027.0
42	86208.5
43	97390.0
44	97390.0
45	97390.0
46	109538.0
47	121686.0
48	121686.0
49	121686.0
50	125494.0
51	129302.0
52	129302.0
53	129302.0
54	122136.0
55	114970.0
56	114970.0
57	114970.0
58	105128.5
59	95287.0
60	95287.0
61	95287.0
62	82589.5
63	69892.0
64	69892.0
65	69892.0
66	56818.5
67	43745.0
68	43745.0
69	43745.0
70	32691.0
71	21637.0
72	21637.0
73	21637.0
74	16366.0
75	11095.0
76	11095.0
77	11095.0
78	8555.5
79	6016.0
80	6016.0
81	6016.0
82	4159.0
83	2302.0
84	2302.0
85	2302.0
86	1721.0
87	1140.0
88	1140.0
89	1140.0
90	830.0
91	520.0
92	520.0
93	520.0
94	369.0
95	218.0
96	218.0
97	218.0
98	379.0
99	540.0
100	540.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03848160151874054
2	0.004810200189842568
3	0.0017102934008329128
4	0.004275733502082282
5	0.00801700031640428
6	0.012185840480934504
7	0.022020027535723755
8	0.03570237474238706
9	0.04949161528660242
10	0.06178434910508898
11	0.05590521553972584
12	0.06573940259451509
13	0.06488425589409863
14	0.06659454929493154
15	0.062318815792849265
16	0.06841173603331652
17	0.0659531892696192
18	0.07974242981383456
19	0.07482533628643993
20	0.07995621648893868
21	0.07942174980117839
22	0.07920796312607428
23	0.08359058996570862
24	0.08027689650159485
25	0.07878038977586604
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	935512.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.97376327081682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91579208601496	49.337403515233085
2	11.62044893430623	14.170850048420073
3	3.197508391610154	5.848923591794633
4	1.3585793502386612	3.31350782744289
5	0.750137940678788	2.286936660770322
6	0.4477368659169494	1.6380121002022512
7	0.3285698229829951	1.4023897023149532
8	0.2151802784503908	1.0496281087026058
9	0.16816386117290297	0.922822511567678
>10	0.8756586888009087	9.43602200500925
>50	0.0629681387934507	2.658581321432817
>100	0.05590487576214248	6.3490564402505205
>500	0.0028216970708034345	1.0559537400701098
>1k	5.29068200775644E-4	0.5299124267887476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2131	0.2277897023234336	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1539	0.16450884649261582	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1258	0.1344718186404878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0689333755205706E-4
2	0.0	0.0	0.0	0.0	1.0689333755205706E-4
3	0.0	0.0	0.0	0.0	1.0689333755205706E-4
4	0.0	0.0	0.0	0.0	1.0689333755205706E-4
5	0.0	0.0	0.0	0.0	1.0689333755205706E-4
6	0.0	0.0	0.0	0.0	2.1378667510411412E-4
7	0.0	0.0	0.0	0.0	2.1378667510411412E-4
8	0.0	0.0	0.0	0.0	2.1378667510411412E-4
9	0.0	0.0	0.0	0.0	2.1378667510411412E-4
10	0.0	0.0	0.0	0.0	3.2068001265617114E-4
11	0.0	0.0	0.0	0.0	3.2068001265617114E-4
12	0.0	0.0	0.0	0.0	7.482533628643994E-4
13	0.0	0.0	0.0	0.0	7.482533628643994E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTC	25	0.0060279663	19.001284	14
TCTTATA	140	0.0	14.245628	2
GCGTTAT	55	1.9621603E-4	13.815421	1
ACCTTAT	55	1.9621603E-4	13.815421	1
CGTTATT	70	7.278173E-6	13.567265	2
AATCGAA	50	0.0014980049	13.300899	7
TAAGGCC	50	0.0014992132	13.299476	4
GTTTTCG	65	5.444503E-5	13.154734	15
GTCCTAT	170	0.0	12.850372	1
TAAGACT	75	1.4776901E-5	12.666167	4
GTATTAC	60	4.0962166E-4	12.664136	1
GTTATAA	60	4.0962166E-4	12.664136	1
TGTACCG	85	3.940302E-6	12.293633	5
CTAGGAC	85	3.949739E-6	12.291003	3
CGCGGTC	55	0.0030640918	12.092373	10
CAGTCCG	55	0.0030640918	12.092373	9
CTATGTT	55	0.0030677773	12.090434	4
AAGAATT	145	9.640644E-11	11.793269	16
TCCTATG	65	8.038844E-4	11.68872	2
CGCCGGT	155	2.5465852E-11	11.645947	7
>>END_MODULE