FastQCFastQC Report
Thu 2 Feb 2017
SRR4064130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935512
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14500.020.9410341
AAGCGAT654.9296446E-420.3085325
CGTCGTA1301.6243575E-920.3063610
TGCGTAG550.004484569719.9986913
ATTATAC1504.656613E-1019.0654143
GTACTAT1051.7892653E-618.8599381
ACCGTCG1404.081812E-918.8559068
ATACCGT1303.587411E-818.6141626
GTGTATA1901.8189894E-1218.5290621
GTATATA1102.791101E-618.0026681
CCGTCGT1355.525726E-817.9247519
GACGGTA2100.017.8083558
GTATAAA1002.47888E-517.602611
GTCCTAA1509.569703E-917.6026081
TATTAGG750.001290679817.5997872
GTCCTAT2400.017.4192471
GGCGTTA1651.6570993E-917.33312442
CATTCGA1408.3671694E-817.28550341
ATAAGGC1154.27347E-617.216263
ATAGGAC3850.017.1417333
Produced by FastQC (version 0.11.5)