##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935512
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323712576642524	32.0	32.0	32.0	32.0	32.0
2	31.416559060706863	32.0	32.0	32.0	32.0	32.0
3	31.4824417003737	32.0	32.0	32.0	32.0	32.0
4	31.561064956943362	32.0	32.0	32.0	32.0	32.0
5	31.50588661609899	32.0	32.0	32.0	32.0	32.0
6	35.10048828876594	36.0	36.0	36.0	36.0	36.0
7	35.125407263616076	36.0	36.0	36.0	36.0	36.0
8	35.06939194793867	36.0	36.0	36.0	36.0	36.0
9	35.17335961484193	36.0	36.0	36.0	36.0	36.0
10	35.03021767759259	36.0	36.0	36.0	36.0	36.0
11	35.17287645695619	36.0	36.0	36.0	36.0	36.0
12	35.077641975730934	36.0	36.0	36.0	36.0	36.0
13	35.1203148650151	36.0	36.0	36.0	36.0	36.0
14	35.057458375734356	36.0	36.0	36.0	36.0	36.0
15	35.04364454972251	36.0	36.0	36.0	36.0	36.0
16	35.05269841541316	36.0	36.0	36.0	36.0	36.0
17	35.0062137097119	36.0	36.0	36.0	36.0	36.0
18	35.01309229598338	36.0	36.0	36.0	36.0	36.0
19	35.00503895193221	36.0	36.0	36.0	36.0	36.0
20	34.994794294461215	36.0	36.0	36.0	36.0	36.0
21	35.00316831852504	36.0	36.0	36.0	36.0	36.0
22	34.973641171893036	36.0	36.0	36.0	36.0	36.0
23	34.92719601672667	36.0	36.0	36.0	36.0	36.0
24	34.90768263795654	36.0	36.0	36.0	32.0	36.0
25	34.866625975936174	36.0	36.0	36.0	32.0	36.0
26	34.816111391409194	36.0	36.0	36.0	32.0	36.0
27	34.80720610745774	36.0	36.0	36.0	32.0	36.0
28	34.784201592283154	36.0	36.0	36.0	32.0	36.0
29	34.76091487869744	36.0	36.0	36.0	32.0	36.0
30	34.74626087105243	36.0	36.0	36.0	32.0	36.0
31	34.73175223834649	36.0	36.0	36.0	32.0	36.0
32	34.69120652647962	36.0	36.0	36.0	32.0	36.0
33	34.67382139406015	36.0	36.0	36.0	32.0	36.0
34	34.66296316883161	36.0	36.0	36.0	32.0	36.0
35	34.61895411282806	36.0	36.0	36.0	32.0	36.0
36	34.59450012399627	36.0	36.0	36.0	32.0	36.0
37	34.57696213410411	36.0	36.0	36.0	32.0	36.0
38	34.538455947117725	36.0	36.0	36.0	32.0	36.0
39	34.54022182505409	36.0	36.0	36.0	32.0	36.0
40	34.511832023533636	36.0	36.0	36.0	32.0	36.0
41	34.494021455630715	36.0	36.0	36.0	32.0	36.0
42	34.43532418611413	36.0	36.0	36.0	32.0	36.0
43	34.42883362265797	36.0	36.0	36.0	32.0	36.0
44	34.37672525846809	36.0	36.0	36.0	32.0	36.0
45	34.34097798852393	36.0	36.0	36.0	32.0	36.0
46	34.320294127707605	36.0	36.0	36.0	32.0	36.0
47	34.27480353004558	36.0	36.0	36.0	32.0	36.0
48	34.23715462762637	36.0	36.0	36.0	32.0	36.0
49	34.228567885820816	36.0	36.0	36.0	32.0	36.0
50	33.620273176613445	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	3.0
21	31.0
22	99.0
23	296.0
24	798.0
25	1768.0
26	3607.0
27	6509.0
28	10715.0
29	16551.0
30	23535.0
31	33331.0
32	47910.0
33	76336.0
34	159452.0
35	554569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78864524626055	17.726141504673777	11.681447716135434	26.80376553293024
2	16.00734574156501	20.496029964468505	36.76494482142323	26.731679472543256
3	18.1436669371071	24.438087847176572	28.801050976418235	28.617194239298094
4	12.402620169490076	15.955220243032691	35.78992038584219	35.85223920163504
5	14.182928706419586	37.04634467542907	33.57562489845133	15.195101719700013
6	34.3357099336191	35.60368141441567	16.635204327051557	13.425404324913684
7	30.06770624000547	30.435846894534755	21.005716655692282	18.490730209767488
8	28.170593397619054	33.120401577319775	19.35028022118393	19.35872480387724
9	27.812001936934195	13.779384050651041	18.129148187224146	40.27946582519062
10	15.551733463602769	27.000086584991724	32.16130035692258	25.28687959448293
11	37.19225408118763	20.692305389989652	22.836906421296575	19.278534107526145
12	24.83490288719522	23.420807901572406	29.183119368045237	22.56116984318714
13	29.977627200535323	19.402230438765553	25.039897980889585	25.580244379809535
14	23.589190483469427	19.240114205208073	25.507825151869195	31.662870159453306
15	25.36473566285255	27.126306292438468	22.172764123351634	25.336193921357346
16	25.638986429789256	25.47383498752011	23.947707387988306	24.939471194702325
17	24.159070113477966	25.64691847886505	25.27279179743285	24.921219610224135
18	24.75390297219149	24.74866515945933	26.21642855997563	24.281003308373553
19	25.734598524229003	24.561122210214524	25.443420761487573	24.2608585040689
20	26.091232300946444	24.27317098199683	25.015339357906235	24.62025735915049
21	26.66946014588803	24.043197735571535	24.54891011553032	24.738432003010118
22	25.881976928142024	24.311606906164755	24.8180675394864	24.988348626206825
23	24.684688344131445	24.195753514419515	25.34101975087199	25.77853839057705
24	25.01298762381	24.552808854245722	25.281932985999024	25.152270535945252
25	25.13597065086329	24.225865209471767	25.516196755517928	25.12196738414702
26	24.775538957713987	24.9521360011032	25.531742035761972	24.740583005420845
27	25.144341523643575	24.69963814400479	24.947218198630612	25.208802133721026
28	24.70795616349324	24.58726894115937	25.442876140061315	25.261898755286076
29	24.856572031465966	24.740055179815432	25.35684622020402	25.046526568514583
30	24.814776411531483	24.75951131544825	25.53151232881984	24.894199944200427
31	25.22278286154109	24.49852475840246	24.879308133071067	25.399384246985374
32	25.007402656404498	24.845452844811458	24.768486597717736	25.378657901066305
33	24.812607165611222	24.552737239542132	25.211872560044814	25.42278303480183
34	25.352943754549763	24.4598279616329	25.32867837034962	24.858549913467716
35	25.649500581514673	24.467550625983446	25.37103629335705	24.51191249914483
36	24.948634127464334	24.959003512762894	25.066866500043826	25.02549585972894
37	25.492952326384362	24.6330784212698	24.841312430116538	25.0326568222293
38	24.952481949554965	24.723497328512757	25.13656762529478	25.187453096637498
39	25.396484341594405	24.60372945422204	24.733610412711442	25.26617579147211
40	25.697170271171654	24.728913859599615	25.137146497686793	24.436769371541942
41	24.916808214741167	24.756250474350846	25.427022658737837	24.899918652170147
42	25.472694465348567	25.074078983375518	25.043506704522134	24.40971984675378
43	24.96715663585608	24.453848793320372	25.274799278675275	25.304195292148275
44	24.793266517299767	24.945590825907747	24.827472677829277	25.433669978963213
45	24.879209495630164	24.967183464742	25.12421112042279	25.02939591920505
46	24.944094306384578	24.648638594810926	24.954142366040124	25.45312473276437
47	25.089845963078172	24.721589755101604	25.166062705106413	25.022501576713807
48	25.361388535835268	25.160531016398913	24.601037532242493	24.877042915523326
49	24.998637108489373	25.00205769894742	24.7056421570671	25.29366303549611
50	24.79438846736832	25.431051991337274	24.78487485863205	24.989684682662354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	72.0
2	47.0
3	128.0
4	209.0
5	218.5
6	228.0
7	201.0
8	174.0
9	249.5
10	325.0
11	494.0
12	663.0
13	1190.5
14	1718.0
15	2142.5
16	2567.0
17	2623.5
18	2680.0
19	2631.0
20	2582.0
21	2860.5
22	3139.0
23	3506.5
24	3874.0
25	4561.0
26	5248.0
27	6509.0
28	7770.0
29	9244.5
30	10719.0
31	12422.5
32	14126.0
33	16614.5
34	19103.0
35	22132.0
36	25161.0
37	29022.0
38	32883.0
39	35809.5
40	38736.0
41	42333.0
42	45930.0
43	48060.5
44	50191.0
45	55065.5
46	59940.0
47	63528.5
48	67117.0
49	69969.5
50	72822.0
51	72787.0
52	72752.0
53	72414.5
54	72077.0
55	71268.5
56	70460.0
57	67896.5
58	65333.0
59	60007.5
60	54682.0
61	47776.5
62	40871.0
63	35305.0
64	29739.0
65	24948.0
66	20157.0
67	17129.5
68	14102.0
69	12363.5
70	10625.0
71	8272.0
72	5919.0
73	5054.0
74	4189.0
75	3274.0
76	2359.0
77	2040.5
78	1722.0
79	1376.5
80	1031.0
81	843.5
82	656.0
83	535.5
84	415.0
85	297.5
86	180.0
87	128.5
88	77.0
89	56.0
90	35.0
91	32.5
92	30.0
93	25.0
94	20.0
95	20.0
96	20.0
97	13.0
98	6.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008551467004164565
2	4.275733502082282E-4
3	1.0689333755205705E-4
4	0.0
5	0.0
6	2.137866751041141E-4
7	0.0
8	1.0689333755205705E-4
9	0.0013896133881767419
10	0.0016034000632808559
11	0.0
12	2.137866751041141E-4
13	1.0689333755205705E-4
14	3.206800126561712E-4
15	0.004275733502082282
16	7.482533628643994E-4
17	0.0
18	7.482533628643994E-4
19	1.0689333755205705E-4
20	0.0010689333755205706
21	0.0
22	0.0
23	9.620400379685135E-4
24	6.413600253123424E-4
25	0.0017102934008329128
26	0.005237773540050796
27	0.006092920240467252
28	0.003848160151874054
29	0.0026723334388014264
30	0.002244760088593198
31	0.007696320303748108
32	0.003955053489426111
33	0.004703306852290511
34	0.0024585467636973122
35	0.0025654401012493696
36	0.007054960278435766
37	0.0027792267763534838
38	0.00833768032906045
39	0.004275733502082282
40	0.0017102934008329128
41	0.0024585467636973122
42	0.0025654401012493696
43	0.0011758267130726277
44	8.551467004164564E-4
45	8.551467004164564E-4
46	8.551467004164564E-4
47	0.0023516534261452554
48	0.0024585467636973122
49	1.0689333755205705E-4
50	0.0010689333755205706
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	935512.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.34335063248875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.01044117639327	52.76826572137766
2	11.179859048755398	14.386991815917414
3	2.960114764147692	5.713911065458851
4	1.2580968595795143	3.238006694622306
5	0.6655357382858423	2.141139968348911
6	0.42444916924684994	1.6386289033511174
7	0.29037888115266947	1.3078765116393192
8	0.21652520570688946	1.1145565785256113
9	0.15242846403742297	0.8826982297138446
>10	0.7437619218135875	8.44644071462043
>50	0.053727451505042906	2.468168157552926
>100	0.04284051609945887	5.1845931005159445
>500	0.001840803276293165	0.708722538355668
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.2068001265617114E-4
13	0.0	0.0	0.0	0.0	5.344666877602853E-4
14	0.0	0.0	0.0	0.0	6.413600253123423E-4
15	0.0	0.0	0.0	1.0689333755205706E-4	0.0011758267130726277
16	0.0	0.0	0.0	1.0689333755205706E-4	0.0011758267130726277
17	0.0	0.0	0.0	1.0689333755205706E-4	0.0011758267130726277
18	0.0	0.0	0.0	1.0689333755205706E-4	0.0011758267130726277
19	0.0	0.0	0.0	2.1378667510411412E-4	0.0011758267130726277
20	0.0	0.0	0.0	2.1378667510411412E-4	0.0012827200506246846
21	0.0	0.0	0.0	2.1378667510411412E-4	0.0012827200506246846
22	0.0	0.0	0.0	2.1378667510411412E-4	0.0013896133881767417
23	0.0	0.0	0.0	4.2757335020822824E-4	0.0016034000632808559
24	0.0	0.0	0.0	0.0014965067257287988	0.0016034000632808559
25	0.0	0.0	0.0	0.002244760088593198	0.0016034000632808559
26	0.0	0.0	0.0	0.0029930134514575975	0.001924080075937027
27	0.0	0.0	0.0	0.003955053489426111	0.001924080075937027
28	0.0	0.0	0.0	0.006841173603331652	0.001924080075937027
29	0.0	0.0	0.0	0.012078947143382448	0.001924080075937027
30	0.0	0.0	0.0	0.02180624086061964	0.001924080075937027
31	0.0	0.0	0.0	0.044895201771863964	0.002030973413489084
32	0.0	0.0	0.0	0.07065649612190972	0.002030973413489084
33	0.0	0.0	0.0	0.09299720367028964	0.002030973413489084
34	0.0	0.0	0.0	0.11907917803299156	0.002030973413489084
35	0.0	0.0	0.0	0.1513609659737128	0.002030973413489084
36	0.0	0.0	0.0	0.19155286089328624	0.002030973413489084
37	0.0	0.0	0.0	0.2653092638042056	0.002030973413489084
38	0.0	0.0	0.0	0.35830646747449524	0.002030973413489084
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1450	0.0	20.941034	1
AAGCGAT	65	4.9296446E-4	20.30853	25
CGTCGTA	130	1.6243575E-9	20.30636	10
TGCGTAG	55	0.0044845697	19.99869	13
ATTATAC	150	4.656613E-10	19.065414	3
GTACTAT	105	1.7892653E-6	18.859938	1
ACCGTCG	140	4.081812E-9	18.855906	8
ATACCGT	130	3.587411E-8	18.614162	6
GTGTATA	190	1.8189894E-12	18.529062	1
GTATATA	110	2.791101E-6	18.002668	1
CCGTCGT	135	5.525726E-8	17.924751	9
GACGGTA	210	0.0	17.808355	8
GTATAAA	100	2.47888E-5	17.60261	1
GTCCTAA	150	9.569703E-9	17.602608	1
TATTAGG	75	0.0012906798	17.599787	2
GTCCTAT	240	0.0	17.419247	1
GGCGTTA	165	1.6570993E-9	17.333124	42
CATTCGA	140	8.3671694E-8	17.285503	41
ATAAGGC	115	4.27347E-6	17.21626	3
ATAGGAC	385	0.0	17.141733	3
>>END_MODULE