FastQCFastQC Report
Thu 2 Feb 2017
SRR4064129_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064129_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT170.8881922675026124No Hit
TATCAACGCAGAGTACTTTTTTTTT100.522466039707419No Hit
GGTATCAACGCAGAGTACTTTTTTT80.41797283176593525No Hit
GTACTTTTTTTTTTTTTTTTTTTTT60.3134796238244514No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG60.3134796238244514No Hit
TTCTAGAGCTAATACATGCCGACGG50.2612330198537095No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50.2612330198537095No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50.2612330198537095No Hit
AGATAACCTCGGGCCGATCGCACGC40.20898641588296762No Hit
CTCCAATAGCGTATATTAAAGTTGC40.20898641588296762No Hit
CATCTAAGGGCATCACAGACCTGTT40.20898641588296762No Hit
ACCATACTCCCCCCGGAACCCAAAG40.20898641588296762No Hit
CTCCCGACCCGGGGAGGTAGTGACG40.20898641588296762No Hit
GCGCAAGACGGACCAGAGCGAAAGC40.20898641588296762No Hit
GAACTACGACGGTATCTGATCGTCT40.20898641588296762No Hit
CCATTATTCCTAGCTGCGGTATCCA40.20898641588296762No Hit
CTCCACTCCTGGTGGTGCCCTTCCG40.20898641588296762No Hit
CCTTCTAGCTGTCCCTATAGCACTT40.20898641588296762No Hit
ATCTGATCGTCTTCGAACCTCCGAC40.20898641588296762No Hit
GTATCTGATCGTCTTCGAACCTCCG30.1567398119122257No Hit
GCTCCACTCCTGGTGGTGCCCTTCC30.1567398119122257No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG30.1567398119122257No Hit
GTTTATGGTCGGAACTACGACGGTA30.1567398119122257No Hit
CGCTATTGGAGCTGGAATTACCGCG30.1567398119122257No Hit
TCGTAGTTCCGACCATAAACGATGC30.1567398119122257No Hit
GCATATGCTTGTCTCAAAGATTAAG30.1567398119122257No Hit
GCAGAGTACTTTTTTTTTTTTTTTT30.1567398119122257No Hit
CTTAGCTGAGTGTCCCGCGGGGCCC30.1567398119122257No Hit
GGTCATGGGAATAACGCCGCCGCAT30.1567398119122257No Hit
GTTATTGCTCAATCTCGGGTGGCTG30.1567398119122257No Hit
GCTTTGAACACTCTAATTTTTTCAA30.1567398119122257No Hit
GTATCAACGCAGAGTACATGGGAGA30.1567398119122257No Hit
CTTTAATATACGCTATTGGAGCTGG30.1567398119122257No Hit
TCTAAGGGCATCACAGACCTGTTAT30.1567398119122257No Hit
GTGTCCCGCGGGGCCCGAAGCGTTT20.10449320794148381No Hit
CCATTTGTCAACCCGGAGCCTTTTC20.10449320794148381No Hit
GTACAGGGAAGTCCTATTTGATGAA20.10449320794148381No Hit
GTGCATGGCCGTTCTTAGTTGGTGG20.10449320794148381No Hit
ACTTGGATAACTGTGGTAATTCTAG20.10449320794148381No Hit
TTCGTATTGCGCCGCTAGAGGTGAA20.10449320794148381No Hit
GTTCTATCAATGGGAGCAGTGTTTG20.10449320794148381No Hit
GTACATGGGGTGGTATCAACGCAAA20.10449320794148381No Hit
ATTCCGGAGAGGGAGCCTGAGAAAC20.10449320794148381No Hit
TTTCGGAACTGAGGCCATGATTAAG20.10449320794148381No Hit
TCTCCGAGGTCACCCCAACCGAAAT20.10449320794148381No Hit
GGACTCTTTCGAGGCCCTGTAATTG20.10449320794148381No Hit
CTTTTTAACTGCAGCAACTTTAATA20.10449320794148381No Hit
GTGTCCAGGTGGTTTACCTGAGGAG20.10449320794148381No Hit
GTGCTGCAGCAGCAGCAGCAGCAAC20.10449320794148381No Hit
CATCGAGGGGGCGCCGAGAGGCAAG20.10449320794148381No Hit
CTATTGGAGCTGGAATTACCGCGGC20.10449320794148381No Hit
ATTTAGGACATTGTATTTAAATTGC20.10449320794148381No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC20.10449320794148381No Hit
TCTGAGGACAGCTTGTTCACCTGAC20.10449320794148381No Hit
GGCATGGCCTACCTGGAAAAAGCTT20.10449320794148381No Hit
GAATAATTACAAATGAATCACTAAC20.10449320794148381No Hit
CTTAGTTGGTGGAGCGATTTGTCTG20.10449320794148381No Hit
AGACGGACCAGAGCGAAAGCATTTG20.10449320794148381No Hit
GTATCAACGCAGAGTACATGGGGAA20.10449320794148381No Hit
AGAAGACCCTATGGAGCTTAAATTA20.10449320794148381No Hit
CCGACTTTCGTTCTTGATTAATGAA20.10449320794148381No Hit
GCGTGTGCCTACCCTACGCCGGCAG20.10449320794148381No Hit
CGTCTGCCCTATCAACTTTCGATGG20.10449320794148381No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT20.10449320794148381No Hit
CCCCGGAACCCAAAGACTTTGGTTT20.10449320794148381No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20.10449320794148381No Hit
GCTTTGATGACACCCACAGCAACTG20.10449320794148381No Hit
ATGCACCACCACCCACGGAATCGAG20.10449320794148381No Hit
GTCAGTGTAGCGCGCGTGCAGCCCC20.10449320794148381No Hit
ATCTAGAGTCACCAAAGCCGCCGGC20.10449320794148381No Hit
GTCTGGCCCTCAACTTTCTCTGTGT20.10449320794148381No Hit
GTGGTGGTGCATGGCCGTTCTTAGT20.10449320794148381No Hit
CTACAGTGGCTGCAAGGCTGCGATG20.10449320794148381No Hit
CCCAAGAGCAGGTCTGGGGCTGTTA20.10449320794148381No Hit
GTACATGGGGAATAATTGCAATCCC20.10449320794148381No Hit
GCACCACCACCCACGGAATCGAGAA20.10449320794148381No Hit
ATTTTGTTGGTTTTCGGAACTGAGG20.10449320794148381No Hit
CTATCAATCTGTCAATCCTGTCCGT20.10449320794148381No Hit
CTCTTAGCTGAGTGTCCCGCGGGGC20.10449320794148381No Hit
CTCCGGAATCGAACCCTGATTCCCC20.10449320794148381No Hit
GTATTTTCACTCTTCTTTTCAGAAT20.10449320794148381No Hit
GGGTAGGCACACGCTGAGCCAGTCA20.10449320794148381No Hit
CTGCCCGGCGGGTCATGGGAATAAC20.10449320794148381No Hit
GTCAATCCTGTCCGTGTCCGGGCCG20.10449320794148381No Hit
CCATGGTAGGCACGGCGACTACCAT20.10449320794148381No Hit
AGTCTGGTGCCAGCAGCCGCGGTAA20.10449320794148381No Hit
GGTATCAACGCAGAGTACATGGGCA20.10449320794148381No Hit
ATCATGGGTCGCATGCACGCTCCCG20.10449320794148381No Hit
ACTGTGGTAATTCTAGAGCTAATAC20.10449320794148381No Hit
GTGTCAGGGTTGTAGCCAATTTTCT20.10449320794148381No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC20.10449320794148381No Hit
GGACCGCGGTTCTATTTTGTTGGTT20.10449320794148381No Hit
TCTTCGAACCTCCGACTTTCGTTCT20.10449320794148381No Hit
CCATTGGGATGTCCTGATCCAACAT20.10449320794148381No Hit
GTACATGGGTACCTGGTTGATCCTG20.10449320794148381No Hit
ATATTAAAGTTGCTGCAGTTAAAAA20.10449320794148381No Hit
GTATCAACGCAGAGTACATGGGAAT20.10449320794148381No Hit
AGCATATGCTTGTCTCAAAGATTAA20.10449320794148381No Hit
GAATAGGACCGCGGTTCTATTTTGT20.10449320794148381No Hit
GTACATGGAAGCAGTGGTATCAACG20.10449320794148381No Hit
GTATCAACGCAGAGTACATGGGAGT20.10449320794148381No Hit
GTACATGGGAGACGAACCCCGCCAG20.10449320794148381No Hit
GGCCTGCTTTGAACACTCTAATTTT20.10449320794148381No Hit
CAACGCAGAGTACTTTTTTTTTTTT20.10449320794148381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)