FastQCFastQC Report
Thu 2 Feb 2017
SRR4064129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG70.3657262277951933No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC70.3657262277951933No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG50.2612330198537095No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT40.20898641588296762No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT40.20898641588296762No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA40.20898641588296762No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG40.20898641588296762No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGC30.1567398119122257No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC30.1567398119122257No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA30.1567398119122257No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA30.1567398119122257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1567398119122257No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC30.1567398119122257No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30.1567398119122257No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCT30.1567398119122257No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG30.1567398119122257No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC30.1567398119122257No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC30.1567398119122257No Hit
GCCTGCCTCCTCACCCGAGTACAGGGTCCATCCACTAAAAGCCCTGTTCT20.10449320794148381No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20.10449320794148381No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC20.10449320794148381No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCA20.10449320794148381No Hit
GGCCTTTCTTGGCCAATTTGTAAATCTGTTCCTTCACGTCGTCAGACGTC20.10449320794148381No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA20.10449320794148381No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC20.10449320794148381No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT20.10449320794148381No Hit
GGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACT20.10449320794148381No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTTGTTCAACGATTAAAG20.10449320794148381No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA20.10449320794148381No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG20.10449320794148381No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20.10449320794148381No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10449320794148381No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC20.10449320794148381No Hit
CTCAATGAGCTGTCTTCAGAATTTATTAATGACCAGAGATATTACTATAG20.10449320794148381No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA20.10449320794148381No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG20.10449320794148381No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA20.10449320794148381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10449320794148381No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA20.10449320794148381No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA20.10449320794148381No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC20.10449320794148381No Hit
GCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT20.10449320794148381No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG20.10449320794148381No Hit
GTGAGGTACAGTGCTGTTTTCCACTGTACAGGAACTTGGGTTTTCGTTCA20.10449320794148381No Hit
GTCATAGATCCCTGTTGTTGTTGTTGCTGTTGTTGCTGCTGCTGTTGCTG20.10449320794148381No Hit
CCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTA20.10449320794148381No Hit
TTAGGGACTAGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTT20.10449320794148381No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGG20.10449320794148381No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTA20.10449320794148381No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG20.10449320794148381No Hit
GGCAAGCCCATGTGTGTTGAGAGCTTCTCTGACTACCCTCCACTTGGTCG20.10449320794148381No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT20.10449320794148381No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA20.10449320794148381No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA20.10449320794148381No Hit
GCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAA20.10449320794148381No Hit
GGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTG20.10449320794148381No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGAC20.10449320794148381No Hit
AGACCGCTGCCGCCGTGACTGGCATGCCTCTCCAGAAGGCTTACGAATAC20.10449320794148381No Hit
ATTTAAGACCTTTATTAACAGGTGCTTGCAGTTTGTTGACTTTTTTGAAA20.10449320794148381No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20.10449320794148381No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC20.10449320794148381No Hit
GCAATGGCTTCATCGAAAGCTGTTTTTGCAAGAGAGCAGGCTTTCTCTGG20.10449320794148381No Hit
TCCAGGCACCGAGGGTGGATGAAACGTGCCTCCTCAACCTCGAGGCTCTT20.10449320794148381No Hit
CGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC20.10449320794148381No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA20.10449320794148381No Hit
CCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC20.10449320794148381No Hit
TCAGTACACCAAACGACCACACATCTGACTTGCTGCTATAGCGACTAAAG20.10449320794148381No Hit
GCCCCAGGGGGACTCTGCGGAGGGACTTCAATCACATCAACGTGGAGCTG20.10449320794148381No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA20.10449320794148381No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG20.10449320794148381No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG20.10449320794148381No Hit
ATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTC20.10449320794148381No Hit
GTATGGAGGAGGTGCTCAAAGAAGTGCGGACCCTCGAGGAAGCAGTGGAC20.10449320794148381No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA20.10449320794148381No Hit
GCATTGAAGAGCTTTGGTTATTTGGCTCATGAAAAAAAATATATAAATAA20.10449320794148381No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT20.10449320794148381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)