FastQCFastQC Report
Thu 2 Feb 2017
SRR4064128_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064128_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637052
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37660.1428109874208017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1351.8189894E-1213.3685941
GTATCAA22000.013.2550561
GGTATCA8500.012.4042331
GGACCGT1551.8189894E-1212.25713356
CGAATTA550.003069418712.09090910
CGTCTAT550.00307247412.0893041
ACTATAC802.8792923E-511.8704953
CGAACGA1052.7150782E-711.7643616
TTGCGCG907.448627E-611.61353618
CGTTATT1657.2759576E-1211.5105642
CGTGCGC752.0748586E-411.40000110
GTCCTAC8250.011.0530771
ATACCGT2250.010.9769456
TGGCGAG6850.010.95849218
TAGCGGC700.001489166810.85961619
CGCATCG1408.352799E-910.8577613
TACCGTC2200.010.7954557
ACGCGCG803.7705642E-410.68810813
CGGTCGG2050.010.65853610
CCGTCGT2150.010.6048539
Produced by FastQC (version 0.11.5)