##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064128_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2637052
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312572524167138	32.0	32.0	32.0	32.0	32.0
2	30.996647013407397	32.0	32.0	32.0	32.0	32.0
3	31.003511496929146	32.0	32.0	32.0	32.0	32.0
4	31.03054016378896	32.0	32.0	32.0	32.0	32.0
5	30.977218120841	32.0	32.0	32.0	32.0	32.0
6	34.61566969479555	36.0	36.0	36.0	32.0	36.0
7	34.57698103791658	36.0	36.0	36.0	32.0	36.0
8	34.559044341939405	36.0	36.0	36.0	32.0	36.0
9	34.664483294224006	36.0	36.0	36.0	32.0	36.0
10	34.43430504972977	36.0	36.0	36.0	32.0	36.0
11	34.61294961191512	36.0	36.0	36.0	32.0	36.0
12	34.489275145124175	36.0	36.0	36.0	32.0	36.0
13	34.537645825717505	36.0	36.0	36.0	32.0	36.0
14	34.46484104219409	36.0	36.0	36.0	32.0	36.0
15	34.41701908039735	36.0	36.0	36.0	32.0	36.0
16	34.41281362673167	36.0	36.0	36.0	32.0	36.0
17	34.36426585444656	36.0	36.0	36.0	32.0	36.0
18	34.350648754745826	36.0	36.0	36.0	32.0	36.0
19	34.361645504146296	36.0	36.0	36.0	32.0	36.0
20	34.3389360543516	36.0	36.0	36.0	32.0	36.0
21	34.33309506221341	36.0	36.0	36.0	32.0	36.0
22	34.31087251976829	36.0	36.0	36.0	32.0	36.0
23	34.26211049308091	36.0	36.0	36.0	32.0	36.0
24	34.250153580589235	36.0	36.0	36.0	32.0	36.0
25	33.86311343121031	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	28.0
5	26.0
6	90.0
7	49.0
8	148.0
9	161.0
10	210.0
11	76.0
12	200.0
13	152.0
14	185.0
15	353.0
16	696.0
17	852.0
18	1047.0
19	1484.0
20	2096.0
21	2978.0
22	4630.0
23	7135.0
24	11164.0
25	16707.0
26	24622.0
27	33419.0
28	46573.0
29	63486.0
30	84360.0
31	115676.0
32	163631.0
33	235459.0
34	522430.0
35	1296925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20151956243158	16.739807179151008	11.268570596148288	26.790102662269135
2	16.562093867881416	19.09611178803748	37.653223326162085	26.688571017919017
3	19.31918114555133	22.518990322718878	28.223425019558203	29.93840351217159
4	13.132629313172675	15.216778772150107	35.074978640103666	36.57561327457355
5	14.93596650211258	35.85709014219005	33.92040345521865	15.28653990047872
6	35.1589107596617	34.64630029961695	16.521333485038117	13.67345545568324
7	30.800330139406523	29.871888917799687	20.351516539969094	18.976264402824693
8	27.979492782073823	33.749888568345064	18.880562336290303	19.39005631329081
9	27.201925794684335	14.344628470727047	18.18111803080285	40.27232770378576
10	15.866196929252915	27.088937076814208	31.93440371236741	25.11046228156546
11	37.08805239054633	20.946543278734552	22.313258131955273	19.652146198763845
12	24.721682664925243	23.142410367190294	29.026964120314595	23.108942847569867
13	30.03690594183387	19.634902107805114	24.618456461916985	25.70973548844403
14	23.320112656559147	19.142393974649995	25.166415838097556	32.3710775306933
15	25.141047750767893	27.310730025745077	21.800599896412574	25.747622327074453
16	25.53938871429341	25.68901126172448	23.783192640832272	24.988407383149838
17	24.32917204219473	25.77430371101161	25.10825681111027	24.788267435683387
18	24.599240378353514	25.390209321000867	25.830096136768326	24.18045416387729
19	25.65163882634615	24.94228843298153	24.97116793487274	24.434904805799583
20	25.885527870778624	24.69261649605197	24.875350192145525	24.546505441023886
21	26.058502497279957	24.45121888741349	24.4328512330047	25.057427382301857
22	25.866164398274144	24.612226511103124	24.72706613601095	24.79454295461178
23	24.85869295011319	24.74358268829712	25.11464534236099	25.283079019228687
24	25.025303442065077	24.708910796400936	25.152209644188257	25.113576117345733
25	25.022827653681535	24.636939735753305	25.175277670038504	25.164954940526655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	76.0
1	76.0
2	242.5
3	409.0
4	409.0
5	409.0
6	1080.5
7	1752.0
8	1752.0
9	1752.0
10	2376.0
11	3000.0
12	3000.0
13	3000.0
14	4361.5
15	5723.0
16	5723.0
17	5723.0
18	10243.0
19	14763.0
20	14763.0
21	14763.0
22	23467.0
23	32171.0
24	32171.0
25	32171.0
26	48117.0
27	64063.0
28	64063.0
29	64063.0
30	85841.0
31	107619.0
32	107619.0
33	107619.0
34	132658.5
35	157698.0
36	157698.0
37	157698.0
38	187598.5
39	217499.0
40	217499.0
41	217499.0
42	251418.0
43	285337.0
44	285337.0
45	285337.0
46	312629.0
47	339921.0
48	339921.0
49	339921.0
50	352237.5
51	364554.0
52	364554.0
53	364554.0
54	345973.5
55	327393.0
56	327393.0
57	327393.0
58	299172.0
59	270951.0
60	270951.0
61	270951.0
62	234391.0
63	197831.0
64	197831.0
65	197831.0
66	161109.0
67	124387.0
68	124387.0
69	124387.0
70	93885.5
71	63384.0
72	63384.0
73	63384.0
74	47431.0
75	31478.0
76	31478.0
77	31478.0
78	23677.0
79	15876.0
80	15876.0
81	15876.0
82	10922.0
83	5968.0
84	5968.0
85	5968.0
86	4320.0
87	2672.0
88	2672.0
89	2672.0
90	1828.5
91	985.0
92	985.0
93	985.0
94	682.0
95	379.0
96	379.0
97	379.0
98	771.0
99	1163.0
100	1163.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03905876713845612
2	0.005043510708169577
3	0.0019339777903507403
4	0.0038300344475573483
5	0.008683939490006265
6	0.01334823886673452
7	0.022373468555037974
8	0.034394467761727866
9	0.04766686436217413
10	0.05828478164253113
11	0.05544069665672122
12	0.06298700215240352
13	0.0646176108776012
14	0.06772714379542004
15	0.06056004963117906
16	0.06651366753480781
17	0.06264571195410633
18	0.07796584974433572
19	0.0745150266282197
20	0.07849674560835358
21	0.07527344929110233
22	0.07861050900778598
23	0.08293351818621704
24	0.07716950594830894
25	0.07925516827123621
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2637052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.13434613790425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.46608545971453	26.944337520992338
2	17.30981260607216	14.9329489706515
3	7.627736631779637	9.870522963718644
4	4.0568848463343254	6.999643008136132
5	2.410406042441513	5.198564428378407
6	1.5172641480502902	3.9267718166780172
7	1.0339858419026975	3.1220212244426304
8	0.6982257616964502	2.4094009349933225
9	0.5199389897199941	2.0184505517857017
>10	2.2037671263925156	15.735486481685701
>50	0.10040077668053528	2.960237661451674
>100	0.050449571635844966	4.079064499502835
>500	0.004334520726222895	1.3210152492355913
>1k	7.076768532567776E-4	0.4815346883475853
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3766	0.1428109874208017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1376339943239648E-4
2	0.0	0.0	0.0	0.0	1.1376339943239648E-4
3	0.0	0.0	0.0	0.0	1.1376339943239648E-4
4	0.0	0.0	0.0	0.0	1.1376339943239648E-4
5	0.0	0.0	0.0	0.0	1.896056657206608E-4
6	0.0	0.0	0.0	0.0	1.896056657206608E-4
7	0.0	0.0	0.0	0.0	2.2752679886479295E-4
8	0.0	0.0	0.0	0.0	2.2752679886479295E-4
9	0.0	0.0	0.0	3.792113314413216E-5	2.2752679886479295E-4
10	0.0	0.0	0.0	7.584226628826432E-5	2.2752679886479295E-4
11	0.0	0.0	0.0	7.584226628826432E-5	2.2752679886479295E-4
12	0.0	0.0	0.0	7.584226628826432E-5	5.308958640178502E-4
13	0.0	0.0	0.0	7.584226628826432E-5	6.446592634502468E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	135	1.8189894E-12	13.368594	1
GTATCAA	2200	0.0	13.255056	1
GGTATCA	850	0.0	12.404233	1
GGACCGT	155	1.8189894E-12	12.2571335	6
CGAATTA	55	0.0030694187	12.090909	10
CGTCTAT	55	0.003072474	12.089304	1
ACTATAC	80	2.8792923E-5	11.870495	3
CGAACGA	105	2.7150782E-7	11.76436	16
TTGCGCG	90	7.448627E-6	11.613536	18
CGTTATT	165	7.2759576E-12	11.510564	2
CGTGCGC	75	2.0748586E-4	11.400001	10
GTCCTAC	825	0.0	11.053077	1
ATACCGT	225	0.0	10.976945	6
TGGCGAG	685	0.0	10.958492	18
TAGCGGC	70	0.0014891668	10.859616	19
CGCATCG	140	8.352799E-9	10.85776	13
TACCGTC	220	0.0	10.795455	7
ACGCGCG	80	3.7705642E-4	10.688108	13
CGGTCGG	205	0.0	10.658536	10
CCGTCGT	215	0.0	10.604853	9
>>END_MODULE