FastQCFastQC Report
Thu 2 Feb 2017
SRR4064128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637052
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24500.027.9268911
GGTATCA10650.022.9298741
TAGGACG8050.018.5830334
GTAGGAC10700.018.2979133
TATCAAC37300.018.1647322
TTAATAC3400.018.1165073
ATCAACG37050.017.872023
TCAACGC37100.017.8482724
CAACGCA37800.017.5177485
AACGCAG38200.017.3343166
GTCCTAA6250.017.2482221
TTAGGAC6800.017.145983
GTATAGA2500.016.7202171
ATTTCGT4400.016.49958642
TAGGACC10950.016.2732564
AGGACGT8950.016.222755
GTACTAC2450.016.1634751
GTCCTAC9550.015.8954931
CTATACT1958.931238E-1015.7941774
TGTAGGA14850.015.5542822
Produced by FastQC (version 0.11.5)