##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064128_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2637052 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.366127402872603 32.0 32.0 32.0 32.0 32.0 2 31.427900928764394 32.0 32.0 32.0 32.0 32.0 3 31.487190241223914 32.0 32.0 32.0 32.0 32.0 4 31.562304421755808 32.0 32.0 32.0 32.0 32.0 5 31.517529802218537 32.0 32.0 32.0 32.0 32.0 6 35.1065739317996 36.0 36.0 36.0 36.0 36.0 7 35.12594480503228 36.0 36.0 36.0 36.0 36.0 8 35.068862123310424 36.0 36.0 36.0 36.0 36.0 9 35.17358815829191 36.0 36.0 36.0 36.0 36.0 10 35.03911489041551 36.0 36.0 36.0 36.0 36.0 11 35.16386821344441 36.0 36.0 36.0 36.0 36.0 12 35.09094359914025 36.0 36.0 36.0 36.0 36.0 13 35.12114929853488 36.0 36.0 36.0 36.0 36.0 14 35.0688606064651 36.0 36.0 36.0 36.0 36.0 15 35.03926202441211 36.0 36.0 36.0 36.0 36.0 16 35.05729352322214 36.0 36.0 36.0 36.0 36.0 17 35.027184901928365 36.0 36.0 36.0 36.0 36.0 18 35.02517508187173 36.0 36.0 36.0 36.0 36.0 19 35.02262298961113 36.0 36.0 36.0 36.0 36.0 20 35.00569423735292 36.0 36.0 36.0 36.0 36.0 21 35.00859672088377 36.0 36.0 36.0 36.0 36.0 22 34.990351346882804 36.0 36.0 36.0 36.0 36.0 23 34.94018017088779 36.0 36.0 36.0 36.0 36.0 24 34.922784988691916 36.0 36.0 36.0 36.0 36.0 25 34.89606765433522 36.0 36.0 36.0 32.0 36.0 26 34.83604532637202 36.0 36.0 36.0 32.0 36.0 27 34.82883121000268 36.0 36.0 36.0 32.0 36.0 28 34.81183723339547 36.0 36.0 36.0 32.0 36.0 29 34.79059608987612 36.0 36.0 36.0 32.0 36.0 30 34.76699549345254 36.0 36.0 36.0 32.0 36.0 31 34.76063763626959 36.0 36.0 36.0 32.0 36.0 32 34.72791018152088 36.0 36.0 36.0 32.0 36.0 33 34.71741778319123 36.0 36.0 36.0 32.0 36.0 34 34.70484920282194 36.0 36.0 36.0 32.0 36.0 35 34.684302774461784 36.0 36.0 36.0 32.0 36.0 36 34.65677354864447 36.0 36.0 36.0 32.0 36.0 37 34.6378804816894 36.0 36.0 36.0 32.0 36.0 38 34.61676106500744 36.0 36.0 36.0 32.0 36.0 39 34.60618524018487 36.0 36.0 36.0 32.0 36.0 40 34.6013093408852 36.0 36.0 36.0 32.0 36.0 41 34.58680905799355 36.0 36.0 36.0 32.0 36.0 42 34.53863139596792 36.0 36.0 36.0 32.0 36.0 43 34.52811093599975 36.0 36.0 36.0 32.0 36.0 44 34.47999356857582 36.0 36.0 36.0 32.0 36.0 45 34.45313592602649 36.0 36.0 36.0 32.0 36.0 46 34.43548591381588 36.0 36.0 36.0 32.0 36.0 47 34.417146495404715 36.0 36.0 36.0 32.0 36.0 48 34.37995799855293 36.0 36.0 36.0 32.0 36.0 49 34.365128560225585 36.0 36.0 36.0 32.0 36.0 50 33.77212887724626 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 3.0 18 2.0 19 8.0 20 23.0 21 59.0 22 225.0 23 760.0 24 1983.0 25 4460.0 26 9418.0 27 17128.0 28 28488.0 29 43995.0 30 64064.0 31 90862.0 32 131165.0 33 210387.0 34 439957.0 35 1594065.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.488800550358654 17.573590369633795 11.75693807630841 27.180671003699143 2 15.98716588409554 19.844605719911083 36.99921957775288 27.169008818240492 3 18.588550530744985 23.094460169098173 28.4944377197533 29.822551580403545 4 12.851699549345252 15.877047551584116 35.052323579512276 36.218929319558356 5 14.608320199980888 36.34437242799915 33.5983894136331 15.448917958386865 6 35.29892694447467 34.93105361333824 16.231021873313694 13.538997568873388 7 30.84499441989468 30.053161737352426 20.25781110461816 18.84403273813474 8 28.047152653796743 33.933953520825526 18.745060772407975 19.273833052969756 9 27.274433745073505 14.26853579742884 17.9958885392938 40.46114191820385 10 15.800145618918323 27.29577325920926 31.938286399041342 24.965794722831074 11 37.40525868120008 20.87196711172223 22.152565234851533 19.57020897222615 12 24.735774924337832 23.227575045002922 28.928526600357745 23.1081234303015 13 30.16745264773739 19.639452356502204 24.623821218000156 25.569273777760248 14 23.433700764947417 19.080986134474813 25.090480098170527 32.39483300240724 15 25.226997699210557 27.323344157826497 21.78836605268038 25.66129209028257 16 25.699216768005726 25.750335037022825 23.690359807388788 24.86008838758266 17 24.424926015869236 25.863047069227303 25.05381008793152 24.658216826971938 18 24.810261466411333 25.41517984111033 25.6458163478129 24.12874234466544 19 25.776359358859818 25.009556125552322 24.92070691059562 24.29337760499224 20 26.034469878389565 24.681696471063816 24.787952493470865 24.495881157075754 21 26.220757118175904 24.463643492809396 24.45044693847524 24.865152450539465 22 25.994957994665242 24.54788426140462 24.715040996630318 24.742116747299818 23 25.005394315964715 24.66372270371838 25.03922018479087 25.291662795526037 24 25.21307057883526 24.696505964838565 25.047885183256813 25.042538273069358 25 25.26048500495259 24.514448973152867 25.12571084457968 25.099355177314862 26 25.00152640953966 25.172332585108236 25.15139896856431 24.674742036787798 27 25.27211980780389 24.98723875474517 24.75427492235171 24.986366515099228 28 25.00884548334262 24.827005857928455 25.093830384396455 25.070318274332475 29 25.044596402556863 24.95483476577787 24.820855947552204 25.179712884113066 30 24.899998179716583 24.94220600147443 25.01175599707541 25.14603982173358 31 25.27401055273885 24.70033384518124 24.809479185589176 25.216176416490732 32 25.204590719962138 24.834355465283668 24.842015766568625 25.119038048185573 33 24.876209979426793 24.87935758506594 24.912767712392274 25.331664723114994 34 25.27922050342361 24.765679276021395 25.078574614028753 24.876525606526243 35 25.169843075047982 24.75895840451605 25.35979428083427 24.7114042396017 36 25.025712712395347 24.97910368063446 24.93787990722186 25.057303699748335 37 25.416636644608353 24.648135156146306 24.826067995221752 25.10916020402359 38 25.111136899574415 24.84885305809458 24.820371975828625 25.21963806650238 39 25.524319855590193 24.66851729656344 24.68543084029216 25.121732007554208 40 25.569786821742174 24.51745263074067 25.027995881675924 24.88476466584123 41 24.990035931322822 24.748167881758455 25.289242408441492 24.972553778477234 42 25.292426102326477 24.819652399268712 25.149460123299622 24.738461375105185 43 25.070648249834658 24.499698142743593 25.21361330720271 25.216040300219035 44 24.672215103493755 24.812525051962083 25.083057801252096 25.43220204329207 45 24.76178521818832 24.79087117952864 25.198833072747895 25.248510529535146 46 24.86460125914572 24.461609403927 25.209193993536662 25.464595343390624 47 25.036282076028726 24.44465765154569 25.30461964590514 25.214440626520446 48 25.01748198710656 24.935912021236252 24.987220326128178 25.05938566552901 49 24.88328765710329 24.77255740245025 25.084649866763442 25.25950507368302 50 24.779418819261657 25.114229709318618 24.929816371641984 25.17653509977774 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 22.0 2 38.0 3 116.0 4 194.0 5 245.0 6 296.0 7 282.0 8 268.0 9 358.0 10 448.0 11 688.0 12 928.0 13 1679.0 14 2430.0 15 3118.5 16 3807.0 17 3797.0 18 3787.0 19 3919.0 20 4051.0 21 4700.0 22 5349.0 23 6845.0 24 8341.0 25 10679.5 26 13018.0 27 16842.0 28 20666.0 29 24983.5 30 29301.0 31 35038.5 32 40776.0 33 49091.0 34 57406.0 35 67462.5 36 77519.0 37 86963.0 38 96407.0 39 104704.5 40 113002.0 41 122517.5 42 132033.0 43 139813.0 44 147593.0 45 159932.5 46 172272.0 47 181539.0 48 190806.0 49 198686.5 50 206567.0 51 206271.5 52 205976.0 53 204153.0 54 202330.0 55 199911.0 56 197492.0 57 189405.0 58 181318.0 59 166063.0 60 150808.0 61 132393.5 62 113979.0 63 99382.5 64 84786.0 65 71623.0 66 58460.0 67 49104.0 68 39748.0 69 33556.0 70 27364.0 71 21512.0 72 15660.0 73 13520.5 74 11381.0 75 9459.5 76 7538.0 77 6289.5 78 5041.0 79 4201.5 80 3362.0 81 2733.5 82 2105.0 83 1655.0 84 1205.0 85 903.5 86 602.0 87 442.5 88 283.0 89 204.5 90 126.0 91 92.5 92 59.0 93 51.5 94 44.0 95 41.5 96 39.0 97 29.5 98 20.0 99 18.5 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008570176090573868 2 6.825803965943789E-4 3 1.5168453257652863E-4 4 0.0 5 0.0 6 1.1376339943239649E-4 7 1.896056657206608E-4 8 0.0 9 0.0012513973937563613 10 0.001213476260612229 11 1.1376339943239649E-4 12 3.792113314413216E-5 13 3.0336906515305727E-4 14 3.0336906515305727E-4 15 0.004967668441881313 16 0.0011376339943239646 17 0.0 18 7.584226628826432E-5 19 0.0 20 9.480283286033039E-4 21 0.0 22 2.2752679886479298E-4 23 6.446592634502468E-4 24 5.688169971619823E-4 25 0.0018202143909183438 26 0.005498564305899163 27 0.00614322356934941 28 0.004285088045286934 29 0.002578637053800987 30 0.0037921133144132157 31 0.007622147761970564 32 0.003033690651530573 33 0.0048159839093047845 34 0.002578637053800987 35 0.0022373468555037974 36 0.008494333824285603 37 0.0038679555807014806 38 0.008115122492844282 39 0.0042471669121428015 40 0.002161504589215533 41 0.002919927252098176 42 0.002313189121792062 43 0.001668529858341815 44 0.0012513973937563613 45 0.0015547664589094185 46 9.859494617474361E-4 47 0.0028440849858099118 48 0.0019718989234948723 49 4.929747308737181E-4 50 0.0015547664589094185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2637052.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.498687203728075 #Duplication Level Percentage of deduplicated Percentage of total 1 65.22191677438526 30.327335069197254 2 16.133226725594476 15.003477262004871 3 7.119414707013674 9.931303126051507 4 3.7894032443481276 7.048091045909437 5 2.261836247970987 5.258620810022842 6 1.4122708245816025 3.9401243591502633 7 0.9200750263449952 2.994759659778446 8 0.6640914775625767 2.4703505511875083 9 0.45527381494963004 1.9052671242051744 >10 1.9067372083804242 14.581847680509426 >50 0.07526887428046636 2.371233505455766 >100 0.037448454744039114 3.178396553888324 >500 0.0027904074238321473 0.8708533631132286 >1k 2.462124197462217E-4 0.11833988952598136 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1376339943239648E-4 2 0.0 0.0 0.0 0.0 1.1376339943239648E-4 3 0.0 0.0 0.0 0.0 1.1376339943239648E-4 4 0.0 0.0 0.0 0.0 1.1376339943239648E-4 5 0.0 0.0 0.0 0.0 1.1376339943239648E-4 6 0.0 0.0 0.0 0.0 1.896056657206608E-4 7 0.0 0.0 0.0 0.0 1.896056657206608E-4 8 0.0 0.0 0.0 0.0 1.896056657206608E-4 9 0.0 0.0 0.0 3.792113314413216E-5 1.896056657206608E-4 10 0.0 0.0 0.0 7.584226628826432E-5 2.2752679886479295E-4 11 0.0 0.0 0.0 7.584226628826432E-5 3.0336906515305727E-4 12 0.0 0.0 0.0 7.584226628826432E-5 0.001213476260612229 13 0.0 0.0 0.0 7.584226628826432E-5 0.0013651607931887578 14 0.0 0.0 0.0 7.584226628826432E-5 0.0013651607931887578 15 0.0 0.0 0.0 7.584226628826432E-5 0.0016685298583418151 16 0.0 0.0 0.0 7.584226628826432E-5 0.0017443721246300794 17 0.0 0.0 0.0 7.584226628826432E-5 0.0018202143909183436 18 0.0 0.0 0.0 7.584226628826432E-5 0.0018581355240624758 19 0.0 0.0 0.0 7.584226628826432E-5 0.001896056657206608 20 0.0 0.0 0.0 7.584226628826432E-5 0.002085662322927269 21 0.0 0.0 0.0 7.584226628826432E-5 0.0022373468555037974 22 0.0 0.0 0.0 7.584226628826432E-5 0.002313189121792062 23 0.0 3.792113314413216E-5 0.0 1.1376339943239648E-4 0.002313189121792062 24 0.0 3.792113314413216E-5 0.0 3.4129019829718944E-4 0.0023511102549361937 25 0.0 3.792113314413216E-5 0.0 3.792113314413216E-4 0.0023511102549361937 26 0.0 3.792113314413216E-5 0.0 4.550535977295859E-4 0.002540715920656855 27 0.0 3.792113314413216E-5 0.0 9.48028328603304E-4 0.0027303215863775155 28 0.0 3.792113314413216E-5 0.0 0.002882006118954044 0.0027682427195216477 29 0.0 3.792113314413216E-5 0.0 0.00807720135970015 0.0027682427195216477 30 0.0 3.792113314413216E-5 0.0 0.017595405778877322 0.0027682427195216477 31 0.0 3.792113314413216E-5 0.0 0.0429267227191576 0.0028440849858099118 32 0.0 3.792113314413216E-5 0.0 0.0700403329172121 0.0029578483852423084 33 0.0 3.792113314413216E-5 0.0 0.09977050130221171 0.0029957695183864407 34 0.0 3.792113314413216E-5 0.0 0.13166217427642685 0.003147454050962969 35 0.0 3.792113314413216E-5 0.0 0.16878696362453224 0.003147454050962969 36 0.0 3.792113314413216E-5 0.0 0.22346923761837081 0.003147454050962969 37 0.0 3.792113314413216E-5 0.0 0.31133250311332505 0.003147454050962969 38 0.0 3.792113314413216E-5 0.0 0.4375340342169969 0.003261217450395366 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2450 0.0 27.926891 1 GGTATCA 1065 0.0 22.929874 1 TAGGACG 805 0.0 18.583033 4 GTAGGAC 1070 0.0 18.297913 3 TATCAAC 3730 0.0 18.164732 2 TTAATAC 340 0.0 18.116507 3 ATCAACG 3705 0.0 17.87202 3 TCAACGC 3710 0.0 17.848272 4 CAACGCA 3780 0.0 17.517748 5 AACGCAG 3820 0.0 17.334316 6 GTCCTAA 625 0.0 17.248222 1 TTAGGAC 680 0.0 17.14598 3 GTATAGA 250 0.0 16.720217 1 ATTTCGT 440 0.0 16.499586 42 TAGGACC 1095 0.0 16.273256 4 AGGACGT 895 0.0 16.22275 5 GTACTAC 245 0.0 16.163475 1 GTCCTAC 955 0.0 15.895493 1 CTATACT 195 8.931238E-10 15.794177 4 TGTAGGA 1485 0.0 15.554282 2 >>END_MODULE