FastQCFastQC Report
Thu 2 Feb 2017
SRR4064127_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064127_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306493
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24770.18959152479194302No Hit
TATCAACGCAGAGTACTTTTTTTTT15890.12162330758756458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCC954.8785296E-914.00395217
CTAGACA852.706256E-713.4083594
ATTTAGA2000.013.2956051
CGATAAC905.4018165E-712.66684510
TAACGAA1001.4392936E-712.35159413
CGGTCCA2250.012.244616510
GGACCTA701.0908891E-412.2139896
ATAGTGT1459.640644E-1111.7928186
CCGATAA1052.7165333E-711.7625229
TAGACAC658.026117E-411.6915775
CAAGACG1950.011.6893384
CCGACCA1501.7644197E-1011.4005989
GTTATAA752.0797066E-411.3962321
CTAACAC1001.9389736E-611.3949233
TATAAGA1251.8464561E-811.3931782
TTGCGCG1604.5474735E-1111.28486618
GTCCTAG1104.986523E-711.2235611
TTTGCGC1701.0913936E-1111.179625517
GCGTTAT855.339066E-511.1727761
ATAGCAC855.3453172E-511.1714933
Produced by FastQC (version 0.11.5)