FastQCFastQC Report
Thu 2 Feb 2017
SRR4064127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306493
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18450.022.0590461
ACCGTCG3050.021.6371238
CGTCGTA3600.020.16459710
ATACCGT3400.020.0567656
GTCGTAG3300.019.99794811
CCGTCGT3350.019.699479
GTCCTAA4550.019.3401551
GTAGGAC9450.019.3207683
TACCGTC3550.019.2092977
ATAATAC1851.8189894E-1219.0250763
GATATAC3200.018.5620161
TACTAGA1203.16586E-718.3314512
TACACTA1456.315531E-918.2050275
TGTAGGA9550.018.1970842
TAGGACG7650.017.8282344
CTATTAT902.2159849E-417.1106641
GGTATCA9250.016.8860451
TAAACGC2350.016.85320528
CAAGACG4350.016.6879434
CTGTAGG9950.016.5824811
Produced by FastQC (version 0.11.5)