##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306493
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33735810295195	32.0	32.0	32.0	32.0	32.0
2	31.429234599802676	32.0	32.0	32.0	32.0	32.0
3	31.498928046304112	32.0	32.0	32.0	32.0	32.0
4	31.57712440862676	32.0	32.0	32.0	32.0	32.0
5	31.53929795261054	32.0	32.0	32.0	32.0	32.0
6	35.137092965672224	36.0	36.0	36.0	36.0	36.0
7	35.152808319677185	36.0	36.0	36.0	36.0	36.0
8	35.098547791683536	36.0	36.0	36.0	36.0	36.0
9	35.20012966009003	36.0	36.0	36.0	36.0	36.0
10	35.06269073006897	36.0	36.0	36.0	36.0	36.0
11	35.204663936201726	36.0	36.0	36.0	36.0	36.0
12	35.111588810655704	36.0	36.0	36.0	36.0	36.0
13	35.15007581364768	36.0	36.0	36.0	36.0	36.0
14	35.09138740123368	36.0	36.0	36.0	36.0	36.0
15	35.065917689570476	36.0	36.0	36.0	36.0	36.0
16	35.078506352502465	36.0	36.0	36.0	36.0	36.0
17	35.04416097139441	36.0	36.0	36.0	36.0	36.0
18	35.04029795796839	36.0	36.0	36.0	36.0	36.0
19	35.038300243476236	36.0	36.0	36.0	36.0	36.0
20	35.02753784367769	36.0	36.0	36.0	36.0	36.0
21	35.02006669764017	36.0	36.0	36.0	36.0	36.0
22	35.00497285481055	36.0	36.0	36.0	36.0	36.0
23	34.95124964312859	36.0	36.0	36.0	36.0	36.0
24	34.92618712844233	36.0	36.0	36.0	36.0	36.0
25	34.89524551604946	36.0	36.0	36.0	32.0	36.0
26	34.835265095182294	36.0	36.0	36.0	32.0	36.0
27	34.823431889799636	36.0	36.0	36.0	32.0	36.0
28	34.79515313132179	36.0	36.0	36.0	32.0	36.0
29	34.775721722198284	36.0	36.0	36.0	32.0	36.0
30	34.746574991216946	36.0	36.0	36.0	32.0	36.0
31	34.74220068534619	36.0	36.0	36.0	32.0	36.0
32	34.705293484159505	36.0	36.0	36.0	32.0	36.0
33	34.692722425608096	36.0	36.0	36.0	32.0	36.0
34	34.67358263687597	36.0	36.0	36.0	32.0	36.0
35	34.64131763430803	36.0	36.0	36.0	32.0	36.0
36	34.60562130834226	36.0	36.0	36.0	32.0	36.0
37	34.5897153677823	36.0	36.0	36.0	32.0	36.0
38	34.55691534512623	36.0	36.0	36.0	32.0	36.0
39	34.533691340099026	36.0	36.0	36.0	32.0	36.0
40	34.530045702502804	36.0	36.0	36.0	32.0	36.0
41	34.52200050057674	36.0	36.0	36.0	32.0	36.0
42	34.44679075969025	36.0	36.0	36.0	32.0	36.0
43	34.443454346866	36.0	36.0	36.0	32.0	36.0
44	34.377549669229	36.0	36.0	36.0	32.0	36.0
45	34.34633480623317	36.0	36.0	36.0	32.0	36.0
46	34.309827147944915	36.0	36.0	36.0	32.0	36.0
47	34.28096974113141	36.0	36.0	36.0	32.0	36.0
48	34.25365310032277	36.0	36.0	36.0	32.0	36.0
49	34.23931471504248	36.0	36.0	36.0	32.0	36.0
50	33.65011217052062	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	4.0
20	14.0
21	48.0
22	127.0
23	448.0
24	1087.0
25	2418.0
26	5009.0
27	8946.0
28	14517.0
29	22294.0
30	32274.0
31	45319.0
32	65355.0
33	105421.0
34	222667.0
35	780542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.116561871824196	17.557614198820847	11.247188757786892	27.078635171568067
2	16.158317929406003	20.227939853882745	37.06540832845383	26.548333888257424
3	18.44228071155702	24.112907361496898	28.553517945452196	28.89129398149388
4	12.322224458914055	15.756379865793388	35.61060028641562	36.31079538887694
5	14.196325583068568	36.99606503823595	33.54078437465795	15.266825004037527
6	34.854560581834214	35.321078095567586	16.49374774682374	13.33061357577446
7	30.46088303640589	30.00745507817882	20.875902799251737	18.65575908616356
8	27.95432348609865	33.737596556274354	19.055914617158006	19.25216534046898
9	27.756371691305354	13.46233625874677	17.900667594348793	40.880624455599076
10	15.804097103370573	26.853141714709096	31.842716787959134	25.500044393961197
11	37.94106661196548	20.256381210141356	22.652186159578473	19.150366018314692
12	25.242251770389718	23.030604090955016	29.03944302758838	22.68770111106689
13	30.411677133683128	19.18881579199149	24.864273221659644	25.53523385266574
14	23.560973630893745	19.42470327005697	24.872961237390843	32.14136186165844
15	25.191857554663905	27.84433704469711	21.568800050212868	25.39500535042612
16	25.705500951791947	25.247860094620446	24.176663607563206	24.869975346024404
17	24.38819036917917	25.826544803531288	25.069479897710895	24.715784929578653
18	25.012916336582506	24.400509764750456	26.468501360520786	24.118072538146247
19	26.260760677630877	24.183137605788932	24.886623962011278	24.66947775456891
20	26.4436817539568	24.02138254830132	24.67205924900841	24.862876448733473
21	26.83274996498259	23.862125552911497	24.077052077584803	25.22807240452111
22	26.331524671811746	24.381645185462432	24.437060798734933	24.84976934399089
23	24.692150841495188	24.15881097663653	25.26276632549652	25.886271856371756
24	25.638868754348486	24.405869471537837	25.01697297106979	24.938288803043886
25	25.33565510247039	24.356761514763008	25.125853919898965	25.18172946286764
26	24.68170966287594	25.166847438789436	25.516425258700597	24.63501763963403
27	25.387146676750227	24.72885763121576	24.702679159870087	25.181316532163926
28	24.787590704509967	24.73890879030036	25.024187869324273	25.449312635865407
29	25.09443392839784	24.46426206821593	24.843834794396194	25.59746920899003
30	24.664260646257592	24.780913088572422	25.36264442820019	25.19218183696979
31	25.633343539497854	24.303735456215556	24.456674831598285	25.6062461726883
32	25.13594111667667	24.760725264227894	24.401353900040416	25.701979719055025
33	24.675988720457795	24.545634076219578	25.235143550851326	25.543233652471304
34	25.5351866876904	24.54059060361588	25.134638641826	24.789584066867718
35	25.613870722805988	24.527503245401327	25.276394910230977	24.582231121561712
36	24.70868216330333	24.985245611765546	25.03813942380191	25.267932801129216
37	25.490128539859757	24.623117885740136	24.520932026885216	25.365821547514894
38	24.989780999548376	24.942551611693293	24.871898896960325	25.19576849179801
39	25.904374815818375	24.129189294694424	24.49674883558944	25.46968705389776
40	26.075688154150207	24.466461431066712	25.005166609770175	24.452683805012914
41	25.32299088912719	24.525110202294595	25.398079238492382	24.75381967008583
42	25.92656094165121	25.216480120875246	24.690176819845217	24.16678211762833
43	25.083200916052036	24.5321766339374	25.098432730490266	25.286189719520298
44	24.85579572592003	24.95767252464638	24.58644602290123	25.600085726532363
45	24.836028996977376	25.169061011985633	24.78949177217273	25.20541821886426
46	24.907327818799573	24.480075194012645	24.894928154662082	25.717668832525696
47	25.486137847682805	24.35209047885487	25.03714204142304	25.124629632039287
48	25.598617341858095	24.991714307802177	24.270686866661208	25.138981483678524
49	25.23666501337938	24.93455737825376	24.369837304284463	25.458940304082393
50	25.013031168411683	25.673353575974584	24.233533846211547	25.08008140940219
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	17.0
2	18.0
3	61.0
4	104.0
5	141.0
6	178.0
7	178.0
8	178.0
9	223.0
10	268.0
11	397.5
12	527.0
13	1012.0
14	1497.0
15	1938.5
16	2380.0
17	2539.0
18	2698.0
19	2711.5
20	2725.0
21	3045.5
22	3366.0
23	3840.0
24	4314.0
25	5068.5
26	5823.0
27	7753.5
28	9684.0
29	12003.0
30	14322.0
31	17448.5
32	20575.0
33	25281.0
34	29987.0
35	35332.5
36	40678.0
37	45531.5
38	50385.0
39	53115.5
40	55846.0
41	59455.5
42	63065.0
43	64834.5
44	66604.0
45	73766.5
46	80929.0
47	86314.5
48	91700.0
49	96172.5
50	100645.0
51	99463.5
52	98282.0
53	97323.5
54	96365.0
55	96276.0
56	96187.0
57	93663.0
58	91139.0
59	83474.5
60	75810.0
61	67190.5
62	58571.0
63	51185.5
64	43800.0
65	37185.0
66	30570.0
67	26272.5
68	21975.0
69	19494.0
70	17013.0
71	13464.0
72	9915.0
73	8377.5
74	6840.0
75	5419.0
76	3998.0
77	3469.0
78	2940.0
79	2432.5
80	1925.0
81	1549.5
82	1174.0
83	966.5
84	759.0
85	550.0
86	341.0
87	255.0
88	169.0
89	123.0
90	77.0
91	64.5
92	52.0
93	44.0
94	36.0
95	27.5
96	19.0
97	16.5
98	14.0
99	12.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010026842853348622
2	6.123262811205265E-4
3	4.592447108403949E-4
4	0.0
5	0.0
6	3.061631405602633E-4
7	7.654078514006582E-5
8	7.654078514006582E-5
9	8.41948636540724E-4
10	6.888670662605923E-4
11	7.654078514006582E-5
12	7.654078514006582E-5
13	6.123262811205265E-4
14	1.5308157028013164E-4
15	0.004209743182703619
16	7.65407851400658E-4
17	0.0
18	6.123262811205265E-4
19	0.0
20	5.357854959804607E-4
21	0.0
22	1.5308157028013164E-4
23	6.888670662605923E-4
24	3.061631405602633E-4
25	0.0013777341325211846
26	0.0045924471084039485
27	0.005817099670645001
28	0.004056661612423488
29	0.0018369788433615795
30	0.003673957686723159
31	0.007118293018026121
32	0.0034443353313029614
33	0.004362824752983751
34	0.0025258459096221715
35	0.0022196827690619085
36	0.007807160084286713
37	0.00382703925700329
38	0.00788370086942678
39	0.0029085498353225007
40	0.002066601198781777
41	0.002449305124482106
42	0.001683897273081448
43	0.0013011933473811188
44	9.950302068208556E-4
45	9.184894216807898E-4
46	4.592447108403949E-4
47	0.001683897273081448
48	0.0018369788433615795
49	3.82703925700329E-4
50	7.65407851400658E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1306493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.721739812818576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.99172701021259	47.1752336786911
2	12.807473851497578	15.297692420372316
3	3.7201364462030164	6.665190627249602
4	1.5820826079129737	3.7793890348865635
5	0.8157505022246557	2.435901962301849
6	0.4905386484258296	1.7577492917651447
7	0.3215128648473457	1.344091536262096
8	0.23039670748265434	1.1007753774407323
9	0.1635931498770872	0.8793060778906953
>10	0.7620542015873288	7.98527409993098
>50	0.05380238134557589	2.237047270508188
>100	0.055122491041116065	6.79301674197511
>500	0.005292769578500931	2.2151209240938203
>1k	5.163677637561884E-4	0.3342109566318638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.65407851400658E-5
2	0.0	0.0	0.0	0.0	7.65407851400658E-5
3	0.0	0.0	0.0	0.0	7.65407851400658E-5
4	0.0	0.0	0.0	0.0	7.65407851400658E-5
5	0.0	0.0	0.0	0.0	1.530815702801316E-4
6	0.0	0.0	0.0	0.0	3.061631405602632E-4
7	0.0	0.0	0.0	0.0	3.061631405602632E-4
8	0.0	0.0	0.0	0.0	3.061631405602632E-4
9	0.0	0.0	0.0	1.530815702801316E-4	3.061631405602632E-4
10	0.0	0.0	0.0	1.530815702801316E-4	3.061631405602632E-4
11	0.0	0.0	0.0	1.530815702801316E-4	3.061631405602632E-4
12	0.0	0.0	0.0	1.530815702801316E-4	6.123262811205264E-4
13	0.0	0.0	0.0	1.530815702801316E-4	6.123262811205264E-4
14	0.0	0.0	0.0	1.530815702801316E-4	6.123262811205264E-4
15	0.0	0.0	0.0	1.530815702801316E-4	7.65407851400658E-4
16	0.0	0.0	0.0	2.2962235542019744E-4	8.419486365407239E-4
17	0.0	0.0	0.0	3.061631405602632E-4	8.419486365407239E-4
18	0.0	0.0	0.0	3.82703925700329E-4	9.184894216807898E-4
19	0.0	0.0	0.0	3.82703925700329E-4	9.184894216807898E-4
20	0.0	0.0	0.0	3.82703925700329E-4	0.0010715709919609214
21	0.0	0.0	0.0	5.357854959804607E-4	0.0012246525622410529
22	0.0	0.0	0.0	6.888670662605923E-4	0.0012246525622410529
23	0.0	0.0	0.0	8.419486365407239E-4	0.0012246525622410529
24	0.0	0.0	0.0	0.0013011933473811188	0.0012246525622410529
25	0.0	0.0	0.0	0.001530815702801316	0.0012246525622410529
26	0.0	0.0	0.0	0.0017604380582215136	0.0013011933473811188
27	0.0	0.0	0.0	0.0022962235542019743	0.0013011933473811188
28	0.0	0.0	0.0	0.00420974318270362	0.0013011933473811188
29	0.0	0.0	0.0	0.007654078514006581	0.0013011933473811188
30	0.0	0.0	0.0	0.014160045250912174	0.0013011933473811188
31	0.0	0.0	0.0	0.030386691700606126	0.0013011933473811188
32	0.0	0.0	0.0	0.04730220521656067	0.0013011933473811188
33	0.0	0.0	0.0	0.06498312658391588	0.0013011933473811188
34	0.0	0.0	0.0	0.08633800563799424	0.0014542749176612503
35	0.0	0.0	0.0	0.108152129402913	0.0014542749176612503
36	0.0	0.0	0.0	0.14297818664164294	0.0014542749176612503
37	0.0	0.0	0.0	0.19456667582604728	0.0014542749176612503
38	0.0	0.0	0.0	0.26383608637780687	0.001530815702801316
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1845	0.0	22.059046	1
ACCGTCG	305	0.0	21.637123	8
CGTCGTA	360	0.0	20.164597	10
ATACCGT	340	0.0	20.056765	6
GTCGTAG	330	0.0	19.997948	11
CCGTCGT	335	0.0	19.69947	9
GTCCTAA	455	0.0	19.340155	1
GTAGGAC	945	0.0	19.320768	3
TACCGTC	355	0.0	19.209297	7
ATAATAC	185	1.8189894E-12	19.025076	3
GATATAC	320	0.0	18.562016	1
TACTAGA	120	3.16586E-7	18.331451	2
TACACTA	145	6.315531E-9	18.205027	5
TGTAGGA	955	0.0	18.197084	2
TAGGACG	765	0.0	17.828234	4
CTATTAT	90	2.2159849E-4	17.110664	1
GGTATCA	925	0.0	16.886045	1
TAAACGC	235	0.0	16.853205	28
CAAGACG	435	0.0	16.687943	4
CTGTAGG	995	0.0	16.582481	1
>>END_MODULE