FastQCFastQC Report
Thu 2 Feb 2017
SRR4064126_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064126_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740145
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30380.41046011254551473No Hit
TATCAACGCAGAGTACTTTTTTTTT23580.3185862229698235No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23540.3180457883252606No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14420.19482668936492176No Hit
GTACATGGGGTGGTATCAACGCAAA12210.16496767525282208No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11200.15132170047760912No Hit
GGTATCAACGCAGAGTACTTTTTTT10590.14308007214802504No Hit
GATTAAGAGGGACGGCCGGGGGCAT9420.12727235879456053No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8530.11524768795303623No Hit
GAATAGGACCGCGGTTCTATTTTGT8080.10916779820170372No Hit
GTATCTGATCGTCTTCGAACCTCCG8000.10808692891257794No Hit
GCGCAAGACGGACCAGAGCGAAAGC7780.10511453836748205No Hit
GTCCTATTCCATTATTCCTAGCTGC7750.10470921238405986No Hit
GTCTTGCGCCGGTCCAAGAATTTCA7620.1029527997892305No Hit
ATCAGATACCGTCGTAGTTCCGACC7440.10052084388869748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA400.005272777814.25116715
CGTTATT801.2872079E-714.2482772
TTAATAC602.5692363E-514.2473143
TAATCCC551.9588534E-413.8174455
CTAGAGA551.9599771E-413.816514
CTTATAC707.2643816E-613.5688693
TAATACT500.001499618413.2983914
ACCGGCG655.44423E-513.1540338
GCGTTAT801.9950457E-613.0626861
TTACACA801.9980762E-613.060924
TTACCGC1750.013.02963716
ATTACCG1800.012.66770415
AATGCCG604.0870297E-412.6668478
CTCTTAA1850.012.3244991
CCGGCGC853.9347997E-612.2942939
AAACGCT701.0885784E-412.21528519
AAGTGCG550.00306550512.0910817
TAGGGTC550.00306861112.0894474
TACCGCG1900.012.00098217
CTACACC802.8651495E-511.8735644
Produced by FastQC (version 0.11.5)