##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064126_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740145
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209866985523107	32.0	32.0	32.0	32.0	32.0
2	30.830480513953347	32.0	32.0	32.0	32.0	32.0
3	30.854470407825495	32.0	32.0	32.0	32.0	32.0
4	30.871833221868688	32.0	32.0	32.0	32.0	32.0
5	30.80191313864175	32.0	32.0	32.0	32.0	32.0
6	34.441840450182056	36.0	36.0	36.0	32.0	36.0
7	34.36238034439198	36.0	36.0	36.0	32.0	36.0
8	34.32792088036803	36.0	36.0	36.0	32.0	36.0
9	34.438312763039676	36.0	36.0	36.0	32.0	36.0
10	34.16324639090989	36.0	36.0	36.0	32.0	36.0
11	34.44364685298151	36.0	36.0	36.0	32.0	36.0
12	34.254823041431074	36.0	36.0	36.0	32.0	36.0
13	34.34973282262259	36.0	36.0	36.0	32.0	36.0
14	34.23624695161083	36.0	36.0	36.0	32.0	36.0
15	34.18502050273933	36.0	36.0	36.0	32.0	36.0
16	34.1729242243074	36.0	36.0	36.0	32.0	36.0
17	34.09416668355525	36.0	36.0	36.0	32.0	36.0
18	34.10469164825811	36.0	36.0	36.0	32.0	36.0
19	34.10936505684697	36.0	36.0	36.0	32.0	36.0
20	34.08486175006249	36.0	36.0	36.0	32.0	36.0
21	34.062644481824506	36.0	36.0	36.0	32.0	36.0
22	34.05772112221254	36.0	36.0	36.0	32.0	36.0
23	34.002744056907765	36.0	36.0	36.0	32.0	36.0
24	33.98350323247472	36.0	36.0	36.0	32.0	36.0
25	33.62097832181532	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	8.0
5	7.0
6	24.0
7	13.0
8	48.0
9	52.0
10	56.0
11	22.0
12	40.0
13	41.0
14	90.0
15	160.0
16	227.0
17	280.0
18	395.0
19	503.0
20	824.0
21	1253.0
22	1888.0
23	2809.0
24	4232.0
25	6145.0
26	8430.0
27	11098.0
28	15326.0
29	20334.0
30	26442.0
31	35580.0
32	50543.0
33	70457.0
34	150554.0
35	332259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.84658469631458	17.114701274969217	11.277118269512787	25.76159575920342
2	16.798767717439773	20.24226783229067	37.16493939926225	25.794025051007306
3	18.85684020515481	24.406045473461205	28.6601974804222	28.076916840961786
4	12.615473168068942	15.207125456184038	35.61187774200217	36.56552363374485
5	14.57114713429242	36.82066373995209	33.1425428906914	15.465646235064096
6	33.67682688708629	35.496315754625066	17.075899643669352	13.750957714619286
7	29.877832885116963	30.547724907766533	21.05315080341095	18.521291403705558
8	28.328359580691025	32.2170772715723	19.83513631866617	19.619426829070502
9	28.009499877669477	13.583824795653953	18.680884943072375	39.72579038360419
10	16.291337369170993	26.19973340759707	31.279454269669298	26.229474953562633
11	37.198849334771666	20.979363186823583	22.760515095722045	19.061272382682702
12	25.10180243027953	23.61464873429701	28.78034469518757	22.503204140235887
13	29.666107407502544	19.186825458414507	25.598071569850838	25.548995564232108
14	23.96154298562391	19.558131013978592	25.39915173917805	31.081174261219445
15	25.08824511525603	27.106493028940072	22.657533246541508	25.147728609262394
16	25.4646880357365	25.41669145363395	24.17175475504167	24.946865755587883
17	23.681264752881628	25.519341991061072	25.613706356480993	25.18568689957631
18	24.674664387784905	24.516458790822455	26.74594074186255	24.06293607953009
19	25.552510279496847	24.38062735691522	25.81455125145522	24.252311112132713
20	25.960967681305025	23.740401140707547	25.36343488731524	24.935196290672188
21	27.160917830631316	23.61913003502035	24.666360182268075	24.553591952080264
22	26.101566112897512	23.767135698233222	25.211280556239164	24.920017632630103
23	24.400926550708096	23.91425754918413	25.704750533118236	25.980065366989535
24	24.818474618898108	24.761954984409776	25.67654543340283	24.74302496328928
25	24.85447290457885	24.26371064683667	25.780843293178446	25.100973155406038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	473.0
1	473.0
2	563.0
3	653.0
4	653.0
5	653.0
6	1765.0
7	2877.0
8	2877.0
9	2877.0
10	2624.5
11	2372.0
12	2372.0
13	2372.0
14	2046.0
15	1720.0
16	1720.0
17	1720.0
18	3016.5
19	4313.0
20	4313.0
21	4313.0
22	7315.0
23	10317.0
24	10317.0
25	10317.0
26	15688.0
27	21059.0
28	21059.0
29	21059.0
30	26615.5
31	32172.0
32	32172.0
33	32172.0
34	38823.0
35	45474.0
36	45474.0
37	45474.0
38	51593.5
39	57713.0
40	57713.0
41	57713.0
42	66506.0
43	75299.0
44	75299.0
45	75299.0
46	85609.5
47	95920.0
48	95920.0
49	95920.0
50	98763.0
51	101606.0
52	101606.0
53	101606.0
54	93329.5
55	85053.0
56	85053.0
57	85053.0
58	78315.5
59	71578.0
60	71578.0
61	71578.0
62	63497.0
63	55416.0
64	55416.0
65	55416.0
66	45979.5
67	36543.0
68	36543.0
69	36543.0
70	27579.5
71	18616.0
72	18616.0
73	18616.0
74	14354.5
75	10093.0
76	10093.0
77	10093.0
78	8087.5
79	6082.0
80	6082.0
81	6082.0
82	4202.5
83	2323.0
84	2323.0
85	2323.0
86	1815.5
87	1308.0
88	1308.0
89	1308.0
90	919.0
91	530.0
92	530.0
93	530.0
94	368.0
95	206.0
96	206.0
97	206.0
98	317.5
99	429.0
100	429.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03837085976396517
2	0.007430976362739733
3	0.002837281883955171
4	0.005134129123347452
5	0.00891717163528768
6	0.013781083436353687
7	0.023644015699626424
8	0.03526336055772855
9	0.04674759675468996
10	0.05931270224077714
11	0.05390835579514825
12	0.06444683136412459
13	0.06431172270298387
14	0.06944585182633133
15	0.05998824554648076
16	0.06877030852062771
17	0.0620148754635916
18	0.08120030534557417
19	0.0764715022056489
20	0.08160563132899634
21	0.0776874801559154
22	0.08201095731241852
23	0.08484823919637369
24	0.07822791480047829
25	0.07998432739530767
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	740145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.382040573945055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.44790368708331	38.51601385355675
2	13.64851530274927	13.752801035144472
3	4.146692106958573	6.267564299413335
4	1.8129816153941911	3.6536685322642626
5	0.9745224291078267	2.4549214281765
6	0.5844926683189808	1.7668759998252181
7	0.4256187691230648	1.501047946649356
8	0.2953048653415863	1.1902449365858543
9	0.2182553192331013	0.9896533514173295
>10	1.2226232195239228	11.074907135566717
>50	0.10898856928413994	3.863404682597247
>100	0.10360626753449197	10.442230819719938
>500	0.008611430028794633	2.819421302753495
>1k	0.001883750318798826	1.7072446763295026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3038	0.41046011254551473	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2358	0.3185862229698235	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2354	0.3180457883252606	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1442	0.19482668936492176	No Hit
GTACATGGGGTGGTATCAACGCAAA	1221	0.16496767525282208	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1120	0.15132170047760912	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1059	0.14308007214802504	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	942	0.12727235879456053	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	853	0.11524768795303623	No Hit
GAATAGGACCGCGGTTCTATTTTGT	808	0.10916779820170372	No Hit
GTATCTGATCGTCTTCGAACCTCCG	800	0.10808692891257794	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	778	0.10511453836748205	No Hit
GTCCTATTCCATTATTCCTAGCTGC	775	0.10470921238405986	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	762	0.1029527997892305	No Hit
ATCAGATACCGTCGTAGTTCCGACC	744	0.10052084388869748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.3510866114072243E-4	0.0
11	0.0	0.0	0.0	2.7021732228144486E-4	0.0
12	0.0	0.0	0.0	2.7021732228144486E-4	0.0
13	0.0	0.0	0.0	2.7021732228144486E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	40	0.0052727778	14.251167	15
CGTTATT	80	1.2872079E-7	14.248277	2
TTAATAC	60	2.5692363E-5	14.247314	3
TAATCCC	55	1.9588534E-4	13.817445	5
CTAGAGA	55	1.9599771E-4	13.81651	4
CTTATAC	70	7.2643816E-6	13.568869	3
TAATACT	50	0.0014996184	13.298391	4
ACCGGCG	65	5.44423E-5	13.154033	8
GCGTTAT	80	1.9950457E-6	13.062686	1
TTACACA	80	1.9980762E-6	13.06092	4
TTACCGC	175	0.0	13.029637	16
ATTACCG	180	0.0	12.667704	15
AATGCCG	60	4.0870297E-4	12.666847	8
CTCTTAA	185	0.0	12.324499	1
CCGGCGC	85	3.9347997E-6	12.294293	9
AAACGCT	70	1.0885784E-4	12.215285	19
AAGTGCG	55	0.003065505	12.091081	7
TAGGGTC	55	0.003068611	12.089447	4
TACCGCG	190	0.0	12.000982	17
CTACACC	80	2.8651495E-5	11.873564	4
>>END_MODULE