FastQCFastQC Report
Thu 2 Feb 2017
SRR4064126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740145
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.13727039971897398No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8860.11970627377068006No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8280.11186997142451818No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8000.10808692891257794No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC7930.10714116828459289No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7830.10579008167318567No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7700.10403366907835628No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7580.10241236514466759No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC7570.10227725648352687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT407.038125E-427.4942574
GTATAAA803.6147176E-621.9983791
GTACTAT856.112052E-620.7043551
CGAGCCG3200.020.6276615
GATATAC1850.020.2147271
CCCGTTG550.00447514820.00528531
GTATATA550.004483908419.9985281
GAACCGT550.004487416219.9958236
ATACACT1950.019.1754824
GTACAAT708.123283E-418.8557531
GTCTTAG1303.5846824E-818.6140141
ACCCGTT600.007398170418.33817930
CGTCGAA600.007409715618.33322122
GCGTAGA600.00741260418.3319821
TGGATAC3500.018.2284624
GTATAGA1102.7954175E-617.9986741
GTATCAA24400.017.7609861
AGGCCCG3600.017.7233110
CCGTCGT3600.017.7221159
GTCGAAC750.00129028217.59989223
Produced by FastQC (version 0.11.5)