##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064126_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740145
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266876085091436	32.0	32.0	32.0	32.0	32.0
2	31.428276891690142	32.0	32.0	32.0	32.0	32.0
3	31.48743962331705	32.0	32.0	32.0	32.0	32.0
4	31.56393004073526	32.0	32.0	32.0	32.0	32.0
5	31.525278154956123	32.0	32.0	32.0	32.0	32.0
6	35.0962689743226	36.0	36.0	36.0	36.0	36.0
7	35.11113092704808	36.0	36.0	36.0	36.0	36.0
8	35.07939930689257	36.0	36.0	36.0	36.0	36.0
9	35.161034662127015	36.0	36.0	36.0	36.0	36.0
10	35.02578413689209	36.0	36.0	36.0	36.0	36.0
11	35.16267623235988	36.0	36.0	36.0	36.0	36.0
12	35.06499131926852	36.0	36.0	36.0	36.0	36.0
13	35.099962845118185	36.0	36.0	36.0	36.0	36.0
14	35.047682548689785	36.0	36.0	36.0	36.0	36.0
15	35.02594896945869	36.0	36.0	36.0	36.0	36.0
16	35.035568706131905	36.0	36.0	36.0	36.0	36.0
17	34.99728161373785	36.0	36.0	36.0	36.0	36.0
18	34.99033567746861	36.0	36.0	36.0	36.0	36.0
19	34.98739301082896	36.0	36.0	36.0	36.0	36.0
20	34.97219869079707	36.0	36.0	36.0	36.0	36.0
21	34.96256409217113	36.0	36.0	36.0	36.0	36.0
22	34.959845705908975	36.0	36.0	36.0	36.0	36.0
23	34.90006958096049	36.0	36.0	36.0	32.0	36.0
24	34.88352822757703	36.0	36.0	36.0	32.0	36.0
25	34.84375358882381	36.0	36.0	36.0	32.0	36.0
26	34.777413885117106	36.0	36.0	36.0	32.0	36.0
27	34.780984807031054	36.0	36.0	36.0	32.0	36.0
28	34.75478588654926	36.0	36.0	36.0	32.0	36.0
29	34.71691763100473	36.0	36.0	36.0	32.0	36.0
30	34.70188003701978	36.0	36.0	36.0	32.0	36.0
31	34.68817191226044	36.0	36.0	36.0	32.0	36.0
32	34.63846543582677	36.0	36.0	36.0	32.0	36.0
33	34.5966128258652	36.0	36.0	36.0	32.0	36.0
34	34.57976207364773	36.0	36.0	36.0	32.0	36.0
35	34.52051152139108	36.0	36.0	36.0	32.0	36.0
36	34.498569874821825	36.0	36.0	36.0	32.0	36.0
37	34.476528247843326	36.0	36.0	36.0	32.0	36.0
38	34.426416445426234	36.0	36.0	36.0	32.0	36.0
39	34.416679164217825	36.0	36.0	36.0	32.0	36.0
40	34.3842355214181	36.0	36.0	36.0	32.0	36.0
41	34.371247525822646	36.0	36.0	36.0	32.0	36.0
42	34.283018867924525	36.0	36.0	36.0	32.0	36.0
43	34.26654371778503	36.0	36.0	36.0	32.0	36.0
44	34.19364313749333	36.0	36.0	36.0	32.0	36.0
45	34.14705091569895	36.0	36.0	36.0	32.0	36.0
46	34.10031142546393	36.0	36.0	36.0	32.0	36.0
47	34.050171250228	36.0	36.0	36.0	32.0	36.0
48	33.99274331381013	36.0	36.0	36.0	32.0	36.0
49	33.949034310844496	36.0	36.0	36.0	32.0	36.0
50	33.35848921495113	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	3.0
20	7.0
21	25.0
22	82.0
23	316.0
24	739.0
25	1709.0
26	3372.0
27	6046.0
28	9533.0
29	14594.0
30	20250.0
31	27778.0
32	39320.0
33	61677.0
34	123819.0
35	430873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10219546206315	17.986493206066438	11.78655590266893	26.124755429201485
2	16.00658795172258	20.930527914351224	36.86888543908644	26.193998694839753
3	18.141359169782056	24.94099113279461	28.942380053584237	27.975269643839095
4	12.243952198555688	15.910395935931474	35.707192509575826	36.138459355937016
5	14.068864884583427	37.083409331955224	33.06460220632444	15.783123577136912
6	33.67205211972838	35.94796674151717	16.78259036779429	13.597390770960166
7	29.65418938181032	30.78086050706281	21.12194232211256	18.443007789014317
8	28.1690749785515	32.378115099068424	19.78612298941424	19.66668693296584
9	27.928462279484584	13.502967677780314	18.632260609802724	39.936309432932376
10	16.100624342184375	26.44814014664813	31.339113328712582	26.112122182454918
11	37.309986556688216	20.897256618635538	22.864708942166736	18.928047882509507
12	25.004323482997904	23.682958991763766	28.805340582373162	22.50737694286517
13	29.671913865060844	19.199314725167234	25.664299099766126	25.4644723100058
14	23.893863896755228	19.417843014332345	25.507739034566246	31.18055405434618
15	25.14578874548717	27.05820200185918	22.713589294593252	25.082419958060402
16	25.42178002180677	25.35665694324159	24.26226495905488	24.95929807589676
17	23.76845077653703	25.523782502077296	25.525538914672126	25.18222780671355
18	24.76099116383387	24.305266571189236	26.975842408193046	23.95789985678385
19	25.7341466874734	24.142026224591127	26.03719541441204	24.08663167352343
20	26.122554861094223	23.479953846694283	25.563067627563356	24.834423664648135
21	27.20737152855184	23.491072695215127	24.893770815178108	24.40778496105493
22	26.083778518176242	23.71544919758641	25.555771730289596	24.645000553947757
23	24.373566153521892	23.594391346525395	26.087156248395576	25.94488625155713
24	24.81814251357851	24.53495284675872	25.91766422568703	24.729240413975734
25	24.944063729517058	23.8437135198236	26.072922521509796	25.13930022914955
26	24.485030935301374	24.97172652626089	26.368848239342896	24.174394299094843
27	25.070328904166228	24.509048850551416	25.622556053690477	24.79806619159188
28	24.35002904973585	24.307467808839224	26.45228411992812	24.890219021496804
29	24.345982667628675	24.27423735134127	26.20406475724141	25.175715223788643
30	24.150841423292327	24.796551887206718	26.294697445667225	24.75790924383373
31	24.865322071776884	24.618328088539506	25.235002344280964	25.28134749540264
32	24.650156664419274	24.863231083734057	25.083196305449	25.40341594639766
33	24.369923889589238	24.306960190998893	25.682973724140634	25.64014219527124
34	25.19621844595261	24.427693384442232	25.79247391297886	24.583614256626298
35	25.793458845529205	24.27112579953899	25.7908916734449	24.144523681486906
36	24.443141297870934	25.249665918558446	25.335331291236596	24.97186149233403
37	25.34102946581058	24.93163196921547	25.30157597768986	24.425762587284087
38	24.646800668025058	24.759219124108895	25.6585667727796	24.93541343508645
39	25.262596100579643	24.55945737795733	25.003985893988734	25.173960627474294
40	25.54953703641151	25.01881444030249	25.389158288554743	24.042490234731254
41	24.342334600698806	25.180309085848474	26.13150875941404	24.34584755403868
42	25.79307120785701	25.55851750167876	25.126295551180945	23.522115739283286
43	24.814256858546077	24.529173731819196	25.568038263289804	25.088531146344923
44	24.344207475663225	25.350510379863135	24.940990494977267	25.364291649496373
45	24.70356639086001	25.47059173228559	24.975544902801122	24.850296974053276
46	24.435205914580678	25.111296962589357	24.98821164189873	25.465285480931232
47	24.821584432920883	25.059652005199112	25.33960433763981	24.779159224240196
48	25.502952184075774	25.572534554740383	24.1893991596071	24.735114101576748
49	24.671887286493675	25.755733359274302	24.603386917645533	24.968992436586493
50	24.804292195737528	26.0243415381539	24.36856028773168	24.802805978376888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	151.5
2	54.0
3	179.0
4	304.0
5	326.0
6	348.0
7	318.5
8	289.0
9	398.5
10	508.0
11	715.5
12	923.0
13	1588.0
14	2253.0
15	2961.0
16	3669.0
17	3806.5
18	3944.0
19	3789.0
20	3634.0
21	3726.5
22	3819.0
23	4116.5
24	4414.0
25	4435.5
26	4457.0
27	5378.5
28	6300.0
29	7262.0
30	8224.0
31	9499.5
32	10775.0
33	12994.5
34	15214.0
35	17666.5
36	20119.0
37	23357.5
38	26596.0
39	28738.0
40	30880.0
41	33479.5
42	36079.0
43	36944.5
44	37810.0
45	41747.0
46	45684.0
47	48618.0
48	51552.0
49	54099.5
50	56647.0
51	55071.0
52	53495.0
53	53074.0
54	52653.0
55	53446.0
56	54239.0
57	52861.0
58	51483.0
59	47206.0
60	42929.0
61	37736.5
62	32544.0
63	28584.0
64	24624.0
65	20765.0
66	16906.0
67	14249.5
68	11593.0
69	10561.5
70	9530.0
71	7469.0
72	5408.0
73	4617.5
74	3827.0
75	2977.0
76	2127.0
77	1850.5
78	1574.0
79	1304.5
80	1035.0
81	847.5
82	660.0
83	532.5
84	405.0
85	297.5
86	190.0
87	132.5
88	75.0
89	56.5
90	38.0
91	29.0
92	20.0
93	20.5
94	21.0
95	15.5
96	10.0
97	8.0
98	6.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009862932263272737
2	8.106519668443346E-4
3	2.7021732228144486E-4
4	0.0
5	0.0
6	4.053259834221673E-4
7	0.0
8	0.0
9	5.404346445628897E-4
10	8.106519668443346E-4
11	0.0
12	1.3510866114072243E-4
13	5.404346445628897E-4
14	1.3510866114072243E-4
15	0.00445858581764384
16	0.0010808692891257795
17	0.0
18	6.755433057036122E-4
19	0.0
20	4.053259834221673E-4
21	0.0
22	4.053259834221673E-4
23	4.053259834221673E-4
24	6.755433057036122E-4
25	0.002296847239392281
26	0.007025650379317566
27	0.0068905417181768435
28	0.004728803139925285
29	0.0036479338507995054
30	0.004053259834221673
31	0.006214998412473231
32	0.0037830425119402284
33	0.004863911801066007
34	0.003512825189658783
35	0.004188368495362395
36	0.007566085023880457
37	0.00445858581764384
38	0.007160759040458289
39	0.004728803139925285
40	0.0029723905450958934
41	0.0036479338507995054
42	0.002161738578251559
43	0.0013510866114072244
44	0.0013510866114072244
45	0.0017564125948293914
46	0.0010808692891257795
47	0.002567064561673726
48	0.0020266299171108363
49	4.053259834221673E-4
50	0.0014861952725479467
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	740145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.896609630356785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92538179664226	42.778392647867804
2	12.970230212307202	14.24043329561776
3	3.982687097617108	6.559080566332351
4	1.6813606473144838	3.6920399641386883
5	0.8822978728988558	2.4217580953111315
6	0.5280412418882379	1.7392604354800434
7	0.37116203853731194	1.4262876277433179
8	0.2635897296949207	1.1576145994906661
9	0.19338759887589038	0.95546911705576
>10	1.0200038537726397	10.023776259245817
>50	0.0927848320701118	3.580846297782072
>100	0.08165032184943515	8.737954664106375
>500	0.0071753313138322285	2.5490851250359725
>1k	2.474252177183527E-4	0.1380013047922709
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1016	0.13727039971897398	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	886	0.11970627377068006	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	828	0.11186997142451818	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	800	0.10808692891257794	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	793	0.10714116828459289	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	783	0.10579008167318567	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	770	0.10403366907835628	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	758	0.10241236514466759	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	757	0.10227725648352687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3510866114072243E-4	0.0
5	0.0	0.0	0.0	1.3510866114072243E-4	0.0
6	0.0	0.0	0.0	1.3510866114072243E-4	1.3510866114072243E-4
7	0.0	0.0	0.0	2.7021732228144486E-4	1.3510866114072243E-4
8	0.0	0.0	0.0	2.7021732228144486E-4	1.3510866114072243E-4
9	0.0	0.0	0.0	2.7021732228144486E-4	1.3510866114072243E-4
10	0.0	0.0	0.0	5.404346445628897E-4	1.3510866114072243E-4
11	0.0	0.0	0.0	6.755433057036121E-4	1.3510866114072243E-4
12	0.0	0.0	0.0	6.755433057036121E-4	5.404346445628897E-4
13	0.0	0.0	0.0	6.755433057036121E-4	5.404346445628897E-4
14	0.0	0.0	0.0	6.755433057036121E-4	5.404346445628897E-4
15	0.0	0.0	0.0	6.755433057036121E-4	5.404346445628897E-4
16	0.0	0.0	0.0	8.106519668443345E-4	5.404346445628897E-4
17	0.0	0.0	0.0	9.45760627985057E-4	5.404346445628897E-4
18	0.0	0.0	0.0	9.45760627985057E-4	5.404346445628897E-4
19	0.0	0.0	0.0	9.45760627985057E-4	5.404346445628897E-4
20	0.0	0.0	0.0	9.45760627985057E-4	0.0012159779502665018
21	0.0	0.0	0.0	0.0010808692891257795	0.0012159779502665018
22	0.0	0.0	0.0	0.001891521255970114	0.0012159779502665018
23	0.0	0.0	0.0	0.0035128251896587833	0.0012159779502665018
24	0.0	0.0	0.0	0.004999020462206729	0.0012159779502665018
25	0.0	0.0	0.0	0.005674563767910342	0.0012159779502665018
26	0.0	0.0	0.0	0.006485215734754676	0.0013510866114072242
27	0.0	0.0	0.0	0.007836302346161901	0.0013510866114072242
28	0.0	0.0	0.0	0.010133149585554183	0.0013510866114072242
29	0.0	0.0	0.0	0.015672604692323802	0.0013510866114072242
30	0.0	0.0	0.0	0.028102601517270266	0.0013510866114072242
31	0.0	0.0	0.0	0.058907376257354975	0.001621303933688669
32	0.0	0.0	0.0	0.09295475886481704	0.001621303933688669
33	0.0	0.0	0.0	0.12119246904322802	0.001621303933688669
34	0.0	0.0	0.0	0.15199724378331272	0.0017564125948293916
35	0.0	0.0	0.0	0.19077342953070006	0.0017564125948293916
36	0.0	0.0	0.0	0.24414135068128542	0.0017564125948293916
37	0.0	0.0	0.0	0.32128839619263794	0.0017564125948293916
38	0.0	0.0	0.0	0.4357254321788298	0.0017564125948293916
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGT	40	7.038125E-4	27.494257	4
GTATAAA	80	3.6147176E-6	21.998379	1
GTACTAT	85	6.112052E-6	20.704355	1
CGAGCCG	320	0.0	20.62766	15
GATATAC	185	0.0	20.214727	1
CCCGTTG	55	0.004475148	20.005285	31
GTATATA	55	0.0044839084	19.998528	1
GAACCGT	55	0.0044874162	19.995823	6
ATACACT	195	0.0	19.175482	4
GTACAAT	70	8.123283E-4	18.855753	1
GTCTTAG	130	3.5846824E-8	18.614014	1
ACCCGTT	60	0.0073981704	18.338179	30
CGTCGAA	60	0.0074097156	18.333221	22
GCGTAGA	60	0.007412604	18.331982	1
TGGATAC	350	0.0	18.22846	24
GTATAGA	110	2.7954175E-6	17.998674	1
GTATCAA	2440	0.0	17.760986	1
AGGCCCG	360	0.0	17.72331	10
CCGTCGT	360	0.0	17.722115	9
GTCGAAC	75	0.001290282	17.599892	23
>>END_MODULE