FastQCFastQC Report
Thu 2 Feb 2017
SRR4064125_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064125_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT271.8789144050104383No Hit
TATCAACGCAGAGTACTTTTTTTTT231.6005567153792624No Hit
GAGTACTTTTTTTTTTTTTTTTTTT191.3221990257480862No Hit
GGTATCAACGCAGAGTACTTTTTTT151.0438413361169103No Hit
GTACTTTTTTTTTTTTTTTTTTTTT151.0438413361169103No Hit
ACGCAGAGTACTTTTTTTTTTTTTT100.6958942240779402No Hit
GTACATGGGGTGGTATCAACGCAAA50.3479471120389701No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG50.3479471120389701No Hit
GTCCTACAGTGGACATTTCTAAATT40.2783576896311761No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40.2783576896311761No Hit
GTATCAACGCAGAGTACATGGGAAG40.2783576896311761No Hit
GTACATGGAAGCAGTGGTATCAACG40.2783576896311761No Hit
TATCAACGCAGAGTACATGGGCAGT30.20876826722338201No Hit
GTACATGGGGAATAATTGCAATCCC30.20876826722338201No Hit
GAACTACGACGGTATCTGATCGTCT30.20876826722338201No Hit
GAGTACATGGAAGCAGTGGTATCAA30.20876826722338201No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT30.20876826722338201No Hit
ATCAACGCAGAGTACTTTTTTTTTT30.20876826722338201No Hit
CCCTTCACCTGACCAATGACTTCCA30.20876826722338201No Hit
AAGTAGGAGAGGAGCGAGCGACCAA30.20876826722338201No Hit
CAACGCAGAGTACTTTTTTTTTTTT30.20876826722338201No Hit
CTTTAGGACGTGAAATATGGCGAGG30.20876826722338201No Hit
TCCATGTACTCTGCGTTGATACCAC20.13917884481558804No Hit
GTATCAACGCAGAGTACATGGGGTG20.13917884481558804No Hit
AGATAACCTCGGGCCGATCGCACGC20.13917884481558804No Hit
CGGTAATTCCAGCTCCAATAGCGTA20.13917884481558804No Hit
TATCAACGCAGAGTACATGGGGCAG20.13917884481558804No Hit
TTCGTATTGCGCCGCTAGAGGTGAA20.13917884481558804No Hit
GGGCAGGCAGTGCCTCTAATACTTG20.13917884481558804No Hit
GCCTTCAACTGGTCTTGCAGGTCTC20.13917884481558804No Hit
GACCAGAGCGAAAGCATTTGCCAAG20.13917884481558804No Hit
GGTATCAACGCAGAGTACATGGGAA20.13917884481558804No Hit
CCCTCGATGCTCTTAGCTGAGTGTC20.13917884481558804No Hit
GTCAATCCTGTCCGTGTCCGGGCCG20.13917884481558804No Hit
ATTCCGGAGAGGGAGCCTGAGAAAC20.13917884481558804No Hit
GTGGTATCAACGCAGAGTACTTTTT20.13917884481558804No Hit
CTCCGAGGTCACCCCAACCGAAATT20.13917884481558804No Hit
CACTTTAGGACGTGAAATATGGCGA20.13917884481558804No Hit
CTCTTTGGCCTCGCCGGTAGAAGCA20.13917884481558804No Hit
GTTGACGTCTGACGACGTGAAGGAA20.13917884481558804No Hit
GCTTTGAACACTCTAATTTTTTCAA20.13917884481558804No Hit
CCATAACTGATTTAATGAGCCATTC20.13917884481558804No Hit
CATTCCAATTACAGGGCCTCGAAAG20.13917884481558804No Hit
AGGTTATCTAGAGTCACCAAAGCCG20.13917884481558804No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA20.13917884481558804No Hit
ACCATACTCCCCCCGGAACCCAAAG20.13917884481558804No Hit
CTCCACTCCTGGTGGTGCCCTTCCG20.13917884481558804No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG20.13917884481558804No Hit
CCCATGTACTCTGCGTTGATACCAC20.13917884481558804No Hit
CGTTTATGGTCGGAACTACGACGGT20.13917884481558804No Hit
TATCAACGCAGAGTACATGGGGTGG20.13917884481558804No Hit
GTATCAACGCAGAGTACATGGGGAG20.13917884481558804No Hit
CCTTTAAGTTTCAGCTTTGCAACCA20.13917884481558804No Hit
GTCCTACACTGGACATTTCTAAATT20.13917884481558804No Hit
GTACATGGGTGGTATCAACGCAAAA20.13917884481558804No Hit
CTTAAATAGGATTGCGCTGTTATCC20.13917884481558804No Hit
GGCGGTGACTCGCCTCGCGGCGGAC20.13917884481558804No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20.13917884481558804No Hit
GGACAACACAGTGAGGCCCTGTCTC20.13917884481558804No Hit
ACCATAAACGATGCCGACTGGCGAT20.13917884481558804No Hit
CCACCGCGCCGTCCGGCTGCACCTT20.13917884481558804No Hit
ATCAACGCAGAGTACATGGGAAGCA20.13917884481558804No Hit
CCATACTCCCCCCGGAACCCAAAGA20.13917884481558804No Hit
AGTGTATACCATGCACAAGCTGGCT20.13917884481558804No Hit
TCGTAGTTCCGACCATAAACGATGC20.13917884481558804No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)