##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064125_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1437
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.16562282533055	32.0	32.0	32.0	14.0	32.0
2	26.667362560890744	32.0	21.0	32.0	14.0	32.0
3	26.730688935281837	32.0	21.0	32.0	14.0	32.0
4	26.416144745998608	32.0	21.0	32.0	14.0	32.0
5	26.58524704244955	32.0	21.0	32.0	14.0	32.0
6	30.09116214335421	36.0	21.0	36.0	14.0	36.0
7	29.95546276965901	36.0	21.0	36.0	14.0	36.0
8	29.927627000695896	36.0	21.0	36.0	14.0	36.0
9	29.848990953375086	36.0	21.0	36.0	14.0	36.0
10	28.73277661795407	36.0	21.0	36.0	14.0	36.0
11	30.393180236604035	36.0	21.0	36.0	14.0	36.0
12	29.29784272790536	36.0	21.0	36.0	14.0	36.0
13	30.239387613082812	36.0	21.0	36.0	14.0	36.0
14	29.554627696590117	36.0	21.0	36.0	14.0	36.0
15	29.346555323590813	36.0	21.0	36.0	14.0	36.0
16	29.313848295059152	36.0	21.0	36.0	14.0	36.0
17	29.366736256089073	36.0	21.0	36.0	14.0	36.0
18	29.459986082115517	36.0	21.0	36.0	14.0	36.0
19	29.157967988865693	36.0	21.0	36.0	14.0	36.0
20	29.249130132219904	36.0	21.0	36.0	14.0	36.0
21	29.356993736951985	36.0	21.0	36.0	14.0	36.0
22	28.987473903966595	36.0	21.0	36.0	14.0	36.0
23	28.97216423103688	36.0	21.0	36.0	14.0	36.0
24	28.755045233124566	36.0	21.0	36.0	14.0	36.0
25	27.72025052192067	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	9.0
16	22.0
17	32.0
18	35.0
19	43.0
20	42.0
21	50.0
22	66.0
23	55.0
24	50.0
25	44.0
26	58.0
27	58.0
28	63.0
29	50.0
30	60.0
31	82.0
32	91.0
33	108.0
34	180.0
35	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43554006968641	18.397212543554005	12.264808362369338	23.902439024390244
2	15.32033426183844	19.91643454038997	40.66852367688023	24.094707520891365
3	18.858733472512178	28.94919972164231	27.41823242867084	24.77383437717467
4	12.178148921363952	15.866388308977037	34.725121781489214	37.2303409881698
5	12.66527487821851	36.74321503131524	33.472512178148925	17.118997912317326
6	32.707028531663184	34.864300626304804	17.049408489909535	15.379262352122478
7	29.018789144050107	31.454418928322898	20.528879610299235	18.997912317327767
8	28.114126652748784	30.340988169798187	19.90257480862909	21.64231036882394
9	28.04453723034099	12.874043145441894	19.137091162143356	39.94432846207376
10	17.4669450243563	26.443980514961723	34.23799582463465	21.851078636047323
11	35.21224773834377	21.08559498956159	23.382045929018787	20.32011134307585
12	25.748086290883787	24.63465553235908	30.758524704244955	18.858733472512178
13	31.454418928322898	21.08559498956159	24.565066109951285	22.89491997216423
14	23.660403618649966	17.049408489909535	28.74043145441893	30.549756437021575
15	23.2428670842032	27.97494780793319	26.722338204592898	22.059846903270703
16	22.616562282533053	24.982602644398053	26.931106471816285	25.469728601252612
17	21.224773834377174	25.748086290883787	28.60125260960334	24.4258872651357
18	23.869171885873346	22.477383437717467	31.524008350730686	22.129436325678498
19	24.07794015309673	22.338204592901878	31.941544885177453	21.64231036882394
20	25.748086290883787	21.224773834377174	32.42867084203201	20.59846903270703
21	26.583159359777316	21.50313152400835	30.549756437021575	21.363952679192764
22	25.191370911621437	23.451635351426585	29.993041057759225	21.363952679192764
23	22.89491997216423	22.268615170494087	30.132219902574807	24.704244954766875
24	22.268615170494087	24.77383437717467	30.2713987473904	22.686151704940848
25	22.477383437717467	24.565066109951285	31.66318719554628	21.29436325678497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	2.5
3	5.0
4	5.0
5	5.0
6	11.0
7	17.0
8	17.0
9	17.0
10	21.0
11	25.0
12	25.0
13	25.0
14	15.5
15	6.0
16	6.0
17	6.0
18	8.5
19	11.0
20	11.0
21	11.0
22	21.0
23	31.0
24	31.0
25	31.0
26	50.5
27	70.0
28	70.0
29	70.0
30	77.0
31	84.0
32	84.0
33	84.0
34	84.5
35	85.0
36	85.0
37	85.0
38	88.5
39	92.0
40	92.0
41	92.0
42	119.0
43	146.0
44	146.0
45	146.0
46	167.5
47	189.0
48	189.0
49	189.0
50	187.5
51	186.0
52	186.0
53	186.0
54	170.0
55	154.0
56	154.0
57	154.0
58	129.0
59	104.0
60	104.0
61	104.0
62	100.0
63	96.0
64	96.0
65	96.0
66	74.0
67	52.0
68	52.0
69	52.0
70	41.5
71	31.0
72	31.0
73	31.0
74	30.0
75	29.0
76	29.0
77	29.0
78	22.5
79	16.0
80	16.0
81	16.0
82	10.5
83	5.0
84	5.0
85	5.0
86	2.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13917884481558804
2	0.06958942240779402
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1437.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.05636743215031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.80415667466026	82.53305497564371
2	3.4372501998401277	5.984690327070286
3	0.7993605115907274	2.0876826722338206
4	0.31974420463629094	1.1134307585247043
5	0.15987210231814547	0.6958942240779402
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.4796163069544364	7.585247042449547
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	27	1.8789144050104383	No Hit
TATCAACGCAGAGTACTTTTTTTTT	23	1.6005567153792624	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	19	1.3221990257480862	No Hit
GGTATCAACGCAGAGTACTTTTTTT	15	1.0438413361169103	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	15	1.0438413361169103	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	10	0.6958942240779402	No Hit
GTACATGGGGTGGTATCAACGCAAA	5	0.3479471120389701	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG	5	0.3479471120389701	No Hit
GTCCTACAGTGGACATTTCTAAATT	4	0.2783576896311761	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4	0.2783576896311761	No Hit
GTATCAACGCAGAGTACATGGGAAG	4	0.2783576896311761	No Hit
GTACATGGAAGCAGTGGTATCAACG	4	0.2783576896311761	No Hit
TATCAACGCAGAGTACATGGGCAGT	3	0.20876826722338201	No Hit
GTACATGGGGAATAATTGCAATCCC	3	0.20876826722338201	No Hit
GAACTACGACGGTATCTGATCGTCT	3	0.20876826722338201	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3	0.20876826722338201	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	3	0.20876826722338201	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3	0.20876826722338201	No Hit
CCCTTCACCTGACCAATGACTTCCA	3	0.20876826722338201	No Hit
AAGTAGGAGAGGAGCGAGCGACCAA	3	0.20876826722338201	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	3	0.20876826722338201	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3	0.20876826722338201	No Hit
TCCATGTACTCTGCGTTGATACCAC	2	0.13917884481558804	No Hit
GTATCAACGCAGAGTACATGGGGTG	2	0.13917884481558804	No Hit
AGATAACCTCGGGCCGATCGCACGC	2	0.13917884481558804	No Hit
CGGTAATTCCAGCTCCAATAGCGTA	2	0.13917884481558804	No Hit
TATCAACGCAGAGTACATGGGGCAG	2	0.13917884481558804	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	2	0.13917884481558804	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	2	0.13917884481558804	No Hit
GCCTTCAACTGGTCTTGCAGGTCTC	2	0.13917884481558804	No Hit
GACCAGAGCGAAAGCATTTGCCAAG	2	0.13917884481558804	No Hit
GGTATCAACGCAGAGTACATGGGAA	2	0.13917884481558804	No Hit
CCCTCGATGCTCTTAGCTGAGTGTC	2	0.13917884481558804	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	2	0.13917884481558804	No Hit
ATTCCGGAGAGGGAGCCTGAGAAAC	2	0.13917884481558804	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2	0.13917884481558804	No Hit
CTCCGAGGTCACCCCAACCGAAATT	2	0.13917884481558804	No Hit
CACTTTAGGACGTGAAATATGGCGA	2	0.13917884481558804	No Hit
CTCTTTGGCCTCGCCGGTAGAAGCA	2	0.13917884481558804	No Hit
GTTGACGTCTGACGACGTGAAGGAA	2	0.13917884481558804	No Hit
GCTTTGAACACTCTAATTTTTTCAA	2	0.13917884481558804	No Hit
CCATAACTGATTTAATGAGCCATTC	2	0.13917884481558804	No Hit
CATTCCAATTACAGGGCCTCGAAAG	2	0.13917884481558804	No Hit
AGGTTATCTAGAGTCACCAAAGCCG	2	0.13917884481558804	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA	2	0.13917884481558804	No Hit
ACCATACTCCCCCCGGAACCCAAAG	2	0.13917884481558804	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	2	0.13917884481558804	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	2	0.13917884481558804	No Hit
CCCATGTACTCTGCGTTGATACCAC	2	0.13917884481558804	No Hit
CGTTTATGGTCGGAACTACGACGGT	2	0.13917884481558804	No Hit
TATCAACGCAGAGTACATGGGGTGG	2	0.13917884481558804	No Hit
GTATCAACGCAGAGTACATGGGGAG	2	0.13917884481558804	No Hit
CCTTTAAGTTTCAGCTTTGCAACCA	2	0.13917884481558804	No Hit
GTCCTACACTGGACATTTCTAAATT	2	0.13917884481558804	No Hit
GTACATGGGTGGTATCAACGCAAAA	2	0.13917884481558804	No Hit
CTTAAATAGGATTGCGCTGTTATCC	2	0.13917884481558804	No Hit
GGCGGTGACTCGCCTCGCGGCGGAC	2	0.13917884481558804	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2	0.13917884481558804	No Hit
GGACAACACAGTGAGGCCCTGTCTC	2	0.13917884481558804	No Hit
ACCATAAACGATGCCGACTGGCGAT	2	0.13917884481558804	No Hit
CCACCGCGCCGTCCGGCTGCACCTT	2	0.13917884481558804	No Hit
ATCAACGCAGAGTACATGGGAAGCA	2	0.13917884481558804	No Hit
CCATACTCCCCCCGGAACCCAAAGA	2	0.13917884481558804	No Hit
AGTGTATACCATGCACAAGCTGGCT	2	0.13917884481558804	No Hit
TCGTAGTTCCGACCATAAACGATGC	2	0.13917884481558804	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE