FastQCFastQC Report
Thu 2 Feb 2017
SRR4064125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA60.41753653444676403No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2783576896311761No Hit
ACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.2783576896311761No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30.20876826722338201No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.20876826722338201No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT30.20876826722338201No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC30.20876826722338201No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.20876826722338201No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC30.20876826722338201No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC20.13917884481558804No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG20.13917884481558804No Hit
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG20.13917884481558804No Hit
ACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTC20.13917884481558804No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT20.13917884481558804No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA20.13917884481558804No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTCTGTCTCTTATACACATCT20.13917884481558804No Hit
TACCATGGTGTACTGCCTTATCACATGCCTAAAATTAACAGGGTGAACCA20.13917884481558804No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAA20.13917884481558804No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20.13917884481558804No Hit
TGATTATGCTACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAG20.13917884481558804No Hit
AATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGC20.13917884481558804No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG20.13917884481558804No Hit
CTCCTGCTCCATCCACCGCGCCCGCGGCTCATATCTCGGATTCTCCGCGT20.13917884481558804No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG20.13917884481558804No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA20.13917884481558804No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG20.13917884481558804No Hit
GCGGGAGATCCGCCTGCAGCCGCAGGAGGCGCCGCTGGCCGCCGAGCCCT20.13917884481558804No Hit
GTAGAGCTCAAGAAGAATGAGTTCCAGGGCGAGCTGCAGAAGCAGAGAGA20.13917884481558804No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT20.13917884481558804No Hit
GAGCAGGGCAGTGGCCGCCAGACCTCGCTCCTCCTGCGCGACGATTGGCC20.13917884481558804No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG20.13917884481558804No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA20.13917884481558804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13917884481558804No Hit
GACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATG20.13917884481558804No Hit
CACTAGAGGCAAGCCTCTTCTGTAGCCTGAGCATACTCATGGCTGCGGCC20.13917884481558804No Hit
GGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGCCGCGTCCTTCGC20.13917884481558804No Hit
TATATATACTGTAAAGTCCGCGTGCACGCAGAGAGCACTCTCCGGGATTG20.13917884481558804No Hit
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCC20.13917884481558804No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA20.13917884481558804No Hit
TAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAG20.13917884481558804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT20.13917884481558804No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG20.13917884481558804No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13917884481558804No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA20.13917884481558804No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13917884481558804No Hit
GACCTGGGCCACCCCGTGTGAGTCCCTCAGGATTACACCTATCTGGGAGG20.13917884481558804No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13917884481558804No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers