##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1437
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.327070285316633	32.0	32.0	32.0	14.0	32.0
2	29.521224773834376	32.0	32.0	32.0	14.0	32.0
3	30.67223382045929	32.0	32.0	32.0	32.0	32.0
4	31.05080027835769	32.0	32.0	32.0	32.0	32.0
5	30.672929714683367	32.0	32.0	32.0	32.0	32.0
6	34.068197633959635	36.0	36.0	36.0	32.0	36.0
7	34.350034794711206	36.0	36.0	36.0	32.0	36.0
8	34.09116214335421	36.0	36.0	36.0	32.0	36.0
9	34.2901878914405	36.0	36.0	36.0	32.0	36.0
10	33.87613082811413	36.0	36.0	36.0	32.0	36.0
11	34.315935977731385	36.0	36.0	36.0	32.0	36.0
12	34.32637439109256	36.0	36.0	36.0	32.0	36.0
13	34.23312456506611	36.0	36.0	36.0	32.0	36.0
14	34.447459986082116	36.0	36.0	36.0	32.0	36.0
15	34.241475295755045	36.0	36.0	36.0	32.0	36.0
16	34.30967292971469	36.0	36.0	36.0	32.0	36.0
17	34.41196938065414	36.0	36.0	36.0	32.0	36.0
18	34.22059846903271	36.0	36.0	36.0	32.0	36.0
19	34.2115518441197	36.0	36.0	36.0	32.0	36.0
20	34.26374391092554	36.0	36.0	36.0	32.0	36.0
21	34.061238691718856	36.0	36.0	36.0	32.0	36.0
22	34.04871259568546	36.0	36.0	36.0	32.0	36.0
23	34.08141962421712	36.0	36.0	36.0	32.0	36.0
24	33.946416144745996	36.0	36.0	36.0	32.0	36.0
25	34.020876826722336	36.0	36.0	36.0	32.0	36.0
26	33.822546972860124	36.0	36.0	36.0	32.0	36.0
27	34.143354210160055	36.0	36.0	36.0	32.0	36.0
28	33.93319415448852	36.0	36.0	36.0	32.0	36.0
29	33.66597077244259	36.0	36.0	36.0	27.0	36.0
30	33.67014613778706	36.0	36.0	36.0	27.0	36.0
31	33.73764787752262	36.0	36.0	36.0	32.0	36.0
32	33.47459986082116	36.0	36.0	36.0	21.0	36.0
33	33.201113430758525	36.0	36.0	36.0	21.0	36.0
34	33.494780793319414	36.0	36.0	36.0	21.0	36.0
35	33.194850382741826	36.0	36.0	36.0	21.0	36.0
36	33.03897007654837	36.0	36.0	36.0	14.0	36.0
37	33.21294363256785	36.0	36.0	36.0	21.0	36.0
38	33.14474599860821	36.0	36.0	36.0	14.0	36.0
39	33.16910229645094	36.0	36.0	36.0	14.0	36.0
40	32.72025052192067	36.0	36.0	36.0	14.0	36.0
41	32.734864300626306	36.0	36.0	36.0	14.0	36.0
42	32.99025748086291	36.0	36.0	36.0	14.0	36.0
43	32.76270006958942	36.0	36.0	36.0	14.0	36.0
44	32.489213639526795	36.0	36.0	36.0	14.0	36.0
45	32.374391092553935	36.0	36.0	36.0	14.0	36.0
46	32.51913709116214	36.0	36.0	36.0	14.0	36.0
47	32.11691022964509	36.0	36.0	36.0	14.0	36.0
48	31.997912317327767	36.0	32.0	36.0	14.0	36.0
49	32.150313152400834	36.0	36.0	36.0	14.0	36.0
50	31.28740431454419	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	5.0
24	5.0
25	15.0
26	17.0
27	25.0
28	41.0
29	59.0
30	86.0
31	103.0
32	132.0
33	220.0
34	314.0
35	415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21503131524008	21.572720946416144	12.038970076548365	23.173277661795407
2	17.536534446764094	25.191370911621437	32.080723729993046	25.191370911621437
3	21.711899791231733	26.026443980514962	28.94919972164231	23.312456506610996
4	14.961725817675713	18.232428670842033	33.6812804453723	33.12456506610995
5	17.18858733472512	38.48295059151009	29.923451635351427	14.40501043841336
6	32.21990257480863	38.48295059151009	14.613778705636744	14.683368128044538
7	28.18371607515658	34.864300626304804	18.928322894919972	18.02366040361865
8	28.18371607515658	33.95963813500348	18.37160751565762	19.485038274182322
9	27.41823242867084	15.727209464161449	16.97981906750174	39.87473903966597
10	17.81489213639527	27.279053583855255	29.923451635351427	24.982602644398053
11	33.95963813500348	23.03409881697982	21.851078636047323	21.15518441196938
12	24.495476687543494	26.722338204592898	28.94919972164231	19.832985386221296
13	31.106471816283925	19.554627696590117	24.704244954766875	24.63465553235908
14	24.843423799582464	22.059846903270703	22.546972860125262	30.549756437021575
15	25.5393180236604	28.18371607515658	22.338204592901878	23.938761308281144
16	24.356297842727905	26.791927627000696	24.63465553235908	24.21711899791232
17	21.851078636047323	29.64509394572025	23.382045929018787	25.12178148921364
18	24.008350730688935	26.65274878218511	26.65274878218511	22.686151704940848
19	26.235212247738342	27.696590118302016	24.77383437717467	21.29436325678497
20	27.00069589422408	27.139874739039666	24.008350730688935	21.851078636047323
21	25.608907446068198	24.91301322199026	23.72999304105776	25.748086290883787
22	23.521224773834376	27.00069589422408	24.28670842032011	25.191370911621437
23	21.64231036882394	29.297146833681282	26.235212247738342	22.825330549756437
24	21.64231036882394	29.575504523312457	23.938761308281144	24.843423799582464
25	23.03409881697982	30.340988169798187	23.521224773834376	23.103688239387612
26	24.21711899791232	27.20946416144746	24.843423799582464	23.72999304105776
27	22.546972860125262	30.410577592205986	23.521224773834376	23.521224773834376
28	22.616562282533053	28.81002087682672	25.887265135699373	22.686151704940848
29	23.799582463465555	29.0883785664579	24.565066109951285	22.546972860125262
30	22.89491997216423	29.784272790535837	25.191370911621437	22.129436325678498
31	23.312456506610996	30.48016701461378	23.938761308281144	22.268615170494087
32	22.477383437717467	30.967292971468336	23.451635351426585	23.103688239387612
33	22.059846903270703	30.549756437021575	24.77383437717467	22.616562282533053
34	23.521224773834376	29.436325678496868	25.191370911621437	21.851078636047323
35	25.748086290883787	30.619345859429366	21.781489213639528	21.851078636047323
36	22.546972860125262	30.758524704244955	24.495476687543494	22.19902574808629
37	21.711899791231733	31.106471816283925	23.521224773834376	23.660403618649966
38	23.59081419624217	30.062630480167012	23.938761308281144	22.407794015309673
39	22.129436325678498	29.366736256089077	25.12178148921364	23.382045929018787
40	22.129436325678498	31.315240083507305	23.173277661795407	23.382045929018787
41	22.546972860125262	30.2713987473904	26.235212247738342	20.946416144746
42	24.843423799582464	30.758524704244955	24.91301322199026	19.485038274182322
43	23.03409881697982	29.993041057759225	24.77383437717467	22.19902574808629
44	23.103688239387612	29.64509394572025	24.356297842727905	22.89491997216423
45	24.4258872651357	30.410577592205986	24.77383437717467	20.389700765483646
46	23.2428670842032	29.15796798886569	23.869171885873346	23.72999304105776
47	23.312456506610996	30.82811412665275	24.14752957550452	21.711899791231733
48	22.059846903270703	32.28949199721642	23.173277661795407	22.477383437717467
49	21.15518441196938	31.66318719554628	24.14752957550452	23.03409881697982
50	21.433542101600555	31.454418928322898	23.799582463465555	23.312456506610996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.0
4	3.0
5	3.5
6	4.0
7	5.5
8	7.0
9	9.5
10	12.0
11	12.0
12	12.0
13	13.0
14	14.0
15	15.0
16	16.0
17	17.5
18	19.0
19	18.0
20	17.0
21	20.5
22	24.0
23	23.0
24	22.0
25	17.5
26	13.0
27	15.0
28	17.0
29	24.5
30	32.0
31	38.5
32	45.0
33	46.0
34	47.0
35	51.5
36	56.0
37	50.0
38	44.0
39	47.5
40	51.0
41	53.0
42	55.0
43	55.5
44	56.0
45	54.0
46	52.0
47	69.0
48	86.0
49	89.0
50	92.0
51	88.0
52	84.0
53	77.5
54	71.0
55	74.0
56	77.0
57	75.5
58	74.0
59	73.5
60	73.0
61	69.0
62	65.0
63	58.0
64	51.0
65	43.0
66	35.0
67	30.0
68	25.0
69	28.5
70	32.0
71	24.5
72	17.0
73	14.5
74	12.0
75	9.5
76	7.0
77	5.5
78	4.0
79	6.0
80	8.0
81	5.5
82	3.0
83	2.0
84	1.0
85	0.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1437.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.75504523312458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.58430232558139	92.48434237995825
2	2.761627906976744	5.288796102992345
3	0.436046511627907	1.2526096033402923
4	0.14534883720930233	0.5567153792623522
5	0.0	0.0
6	0.07267441860465117	0.41753653444676403
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	6	0.41753653444676403	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.2783576896311761	No Hit
ACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.2783576896311761	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3	0.20876826722338201	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.20876826722338201	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	3	0.20876826722338201	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC	3	0.20876826722338201	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.20876826722338201	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3	0.20876826722338201	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC	2	0.13917884481558804	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2	0.13917884481558804	No Hit
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	2	0.13917884481558804	No Hit
ACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTC	2	0.13917884481558804	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT	2	0.13917884481558804	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	2	0.13917884481558804	No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTCTGTCTCTTATACACATCT	2	0.13917884481558804	No Hit
TACCATGGTGTACTGCCTTATCACATGCCTAAAATTAACAGGGTGAACCA	2	0.13917884481558804	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAA	2	0.13917884481558804	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2	0.13917884481558804	No Hit
TGATTATGCTACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAG	2	0.13917884481558804	No Hit
AATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGC	2	0.13917884481558804	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG	2	0.13917884481558804	No Hit
CTCCTGCTCCATCCACCGCGCCCGCGGCTCATATCTCGGATTCTCCGCGT	2	0.13917884481558804	No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG	2	0.13917884481558804	No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA	2	0.13917884481558804	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	2	0.13917884481558804	No Hit
GCGGGAGATCCGCCTGCAGCCGCAGGAGGCGCCGCTGGCCGCCGAGCCCT	2	0.13917884481558804	No Hit
GTAGAGCTCAAGAAGAATGAGTTCCAGGGCGAGCTGCAGAAGCAGAGAGA	2	0.13917884481558804	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT	2	0.13917884481558804	No Hit
GAGCAGGGCAGTGGCCGCCAGACCTCGCTCCTCCTGCGCGACGATTGGCC	2	0.13917884481558804	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	2	0.13917884481558804	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA	2	0.13917884481558804	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.13917884481558804	No Hit
GACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATG	2	0.13917884481558804	No Hit
CACTAGAGGCAAGCCTCTTCTGTAGCCTGAGCATACTCATGGCTGCGGCC	2	0.13917884481558804	No Hit
GGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGCCGCGTCCTTCGC	2	0.13917884481558804	No Hit
TATATATACTGTAAAGTCCGCGTGCACGCAGAGAGCACTCTCCGGGATTG	2	0.13917884481558804	No Hit
CAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCC	2	0.13917884481558804	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2	0.13917884481558804	No Hit
TAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAG	2	0.13917884481558804	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT	2	0.13917884481558804	No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG	2	0.13917884481558804	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.13917884481558804	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA	2	0.13917884481558804	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.13917884481558804	No Hit
GACCTGGGCCACCCCGTGTGAGTCCCTCAGGATTACACCTATCTGGGAGG	2	0.13917884481558804	No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.13917884481558804	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.2783576896311761	0.0
33	0.0	0.0	0.0	0.2783576896311761	0.0
34	0.0	0.0	0.0	0.2783576896311761	0.0
35	0.0	0.0	0.0	0.4175365344467641	0.0
36	0.0	0.0	0.0	0.5567153792623522	0.0
37	0.0	0.0	0.0	0.6263048016701461	0.0
38	0.0	0.0	0.0	0.6263048016701461	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE