FastQCFastQC Report
Thu 2 Feb 2017
SRR4064124_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064124_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405458
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT38400.2732205444773163No Hit
GTCCTACAGTGGACATTTCTAAATT35510.25265785245805994No Hit
CTGTAGGACGTGGAATATGGCAAGA33530.23856991813344833No Hit
GATATACACTGTTCTACAAATCCCG32730.23287782345683758No Hit
CTTTAGGACGTGAAATATGGCGAGG32600.2319528580718883No Hit
GTATCAACGCAGAGTACTTTTTTTT32200.22910681073358294No Hit
TTGTAGAACAGTGTATATCAATGAG29870.2125285849879541No Hit
GTCCTACAGTGTGCATTTCTCATTT29140.2073345485955468No Hit
GTGTATATCAATGAGTTACAATGAG24780.1763126326080182No Hit
GTGTATATCAATGAGTTACAATGAA23460.16692067639161043No Hit
TATCAACGCAGAGTACTTTTTTTTT22690.16144203526537257No Hit
CTGTAGGACCTGGAATATGGCGAGA22270.1584536855601519No Hit
GTTCTACAGTGTGGTTTTTATCATT17630.12543953643580952No Hit
GATATACACTGTTCTACAATGCCGG16220.11540721956828308No Hit
GTATCAACGCAGAGTACATGGGCAG16180.11512261483445255No Hit
GTTCTAAGTTGGTTGTTAAGCGTAC14630.10409418139851921No Hit
GTTCTACAAATCCCGTTTCCAACGA14450.1028134600962818No Hit
CTGAAGGACCTGGAATATGGCGAGA14180.10089237814292565No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14090.10025201749180694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT350.002167750616.28831115
GACCGTT653.3672386E-614.6171927
AAGCGTA2200.014.25379418
AATTCGC400.00527069714.25328619
CCGCGCG602.5631947E-514.2527789
AGCGTAC2250.013.93654619
TAGAAAT3700.013.6049474
AGACCGT707.2600833E-613.5716586
AACGTAA852.6943235E-713.413424510
TTGTAGA5750.013.2100871
ACCGTTA655.4417935E-513.1564118
TCCAACG4900.012.99325518
GCGTTAT957.4382115E-812.9928161
CGTTATT1102.7757778E-912.9446212
GCCCGTA1550.012.87301917
TGTAGAA10950.012.7436552
CCCGTAA1501.8189894E-1212.67003918
GTTAAGC3000.012.66868515
TTAAGCG3000.012.66868516
ATTCCCG905.397942E-712.66778211
Produced by FastQC (version 0.11.5)