##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064124_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1405458
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280858624021494	32.0	32.0	32.0	32.0	32.0
2	30.91239510536779	32.0	32.0	32.0	32.0	32.0
3	30.921676777249836	32.0	32.0	32.0	32.0	32.0
4	30.956375786398453	32.0	32.0	32.0	32.0	32.0
5	30.873826183350907	32.0	32.0	32.0	32.0	32.0
6	34.53540482888852	36.0	36.0	36.0	32.0	36.0
7	34.47284870839257	36.0	36.0	36.0	32.0	36.0
8	34.433647252354746	36.0	36.0	36.0	32.0	36.0
9	34.56592014844983	36.0	36.0	36.0	32.0	36.0
10	34.286199943363656	36.0	36.0	36.0	32.0	36.0
11	34.53215820038735	36.0	36.0	36.0	32.0	36.0
12	34.37654771611816	36.0	36.0	36.0	32.0	36.0
13	34.43747305148926	36.0	36.0	36.0	32.0	36.0
14	34.3652183131762	36.0	36.0	36.0	32.0	36.0
15	34.29280988830687	36.0	36.0	36.0	32.0	36.0
16	34.28909935408956	36.0	36.0	36.0	32.0	36.0
17	34.23133313126397	36.0	36.0	36.0	32.0	36.0
18	34.230797362852535	36.0	36.0	36.0	32.0	36.0
19	34.20685997020188	36.0	36.0	36.0	32.0	36.0
20	34.174115484062845	36.0	36.0	36.0	32.0	36.0
21	34.154338301108965	36.0	36.0	36.0	32.0	36.0
22	34.12377032967189	36.0	36.0	36.0	32.0	36.0
23	34.08051823675983	36.0	36.0	36.0	32.0	36.0
24	34.03743263761706	36.0	36.0	36.0	32.0	36.0
25	33.66740521595096	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	6.0
5	21.0
6	54.0
7	21.0
8	84.0
9	82.0
10	138.0
11	37.0
12	90.0
13	68.0
14	119.0
15	203.0
16	384.0
17	543.0
18	701.0
19	906.0
20	1329.0
21	1881.0
22	3012.0
23	4388.0
24	6632.0
25	9844.0
26	14282.0
27	19159.0
28	26611.0
29	35931.0
30	48610.0
31	66559.0
32	94551.0
33	135366.0
34	294742.0
35	639096.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.125807167993436	17.164633799127632	11.208887104672748	25.50067192820618
2	15.426613243507287	19.181888495876212	41.052510240980574	24.33898801963593
3	20.041183242660946	23.53713393661424	28.822648791185024	27.599034029539798
4	11.768749110573502	15.022840472463356	36.92934395901523	36.27906645794792
5	13.169869831192555	37.93552968735435	34.63808118952929	14.256519291923814
6	33.446075044279524	36.990856606918065	16.766943456699284	12.796124892103123
7	29.175340124072253	30.89999708215997	20.33898184463	19.585680949137778
8	26.987388731427952	36.39651399994591	18.730559585005416	17.885537683620726
9	27.363575077932182	13.245730369952739	18.471724801690268	40.91896975042481
10	15.370103136085955	27.9319602263626	32.43529166361134	24.26264497394011
11	38.00756764068301	20.18979614356448	23.52503977816775	18.277596437584762
12	25.06302690025837	22.971267137941517	31.30662364485935	20.659082316940765
13	30.912341356237743	20.36464036475428	25.230356509716195	23.49266176929178
14	22.09018771604433	21.11938996735528	25.34155918591115	31.44886313068924
15	24.502416366934646	30.80974634554759	21.887485867741997	22.80035141977576
16	23.98427932674508	25.882863896562526	27.37938939993734	22.753467376755047
17	22.190310063721476	26.830728703143354	27.088391299704533	23.890569933430637
18	22.633143257530875	26.123009046165063	29.409934261495696	21.833913434808366
19	25.899664265853023	24.196285206691897	24.91182973329963	24.992220794155447
20	25.614131030760312	26.117284820933932	25.877243577419172	22.391340570886577
21	25.8688531594013	24.007248750338228	24.69210612512283	25.43179196513764
22	25.420962572480615	26.427698116055858	25.35288793818595	22.798451373277572
23	24.139674455633934	25.93260385645741	25.92021328867032	24.007508399238336
24	25.232618666317276	25.59328708137719	26.18979632381686	22.984297928488676
25	24.510220691747183	25.9084406600316	26.328134512805505	23.253204135415707
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	68.0
2	234.5
3	401.0
4	401.0
5	401.0
6	1211.5
7	2022.0
8	2022.0
9	2022.0
10	2197.5
11	2373.0
12	2373.0
13	2373.0
14	2638.0
15	2903.0
16	2903.0
17	2903.0
18	5293.5
19	7684.0
20	7684.0
21	7684.0
22	14439.5
23	21195.0
24	21195.0
25	21195.0
26	37509.5
27	53824.0
28	53824.0
29	53824.0
30	75870.5
31	97917.0
32	97917.0
33	97917.0
34	104838.0
35	111759.0
36	111759.0
37	111759.0
38	120521.0
39	129283.0
40	129283.0
41	129283.0
42	139371.5
43	149460.0
44	149460.0
45	149460.0
46	164439.5
47	179419.0
48	179419.0
49	179419.0
50	177855.5
51	176292.0
52	176292.0
53	176292.0
54	159105.5
55	141919.0
56	141919.0
57	141919.0
58	127964.0
59	114009.0
60	114009.0
61	114009.0
62	101368.5
63	88728.0
64	88728.0
65	88728.0
66	73800.0
67	58872.0
68	58872.0
69	58872.0
70	46338.5
71	33805.0
72	33805.0
73	33805.0
74	25813.5
75	17822.0
76	17822.0
77	17822.0
78	13393.5
79	8965.0
80	8965.0
81	8965.0
82	6160.0
83	3355.0
84	3355.0
85	3355.0
86	2500.0
87	1645.0
88	1645.0
89	1645.0
90	1163.0
91	681.0
92	681.0
93	681.0
94	492.0
95	303.0
96	303.0
97	303.0
98	528.5
99	754.0
100	754.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03884854616786841
2	0.0053363387593225835
3	0.0022768378706443026
4	0.004126768640542798
5	0.009178502666034843
6	0.012878364205831837
7	0.021985715688409044
8	0.03728322013180045
9	0.0505884914383781
10	0.05756130741722627
11	0.056422888481904125
12	0.06396491392841337
13	0.06375146037804047
14	0.06709556600054928
15	0.06190152960814197
16	0.06673981008326112
17	0.06460527457953208
18	0.0786932089041437
19	0.07634521985004177
20	0.07940472073872006
21	0.07670097576732994
22	0.07947587192217767
23	0.0828199775446865
24	0.07976047665600822
25	0.07833745298685553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1405458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.51992805218008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03016423193807	33.24327821476716
2	15.022641318948812	13.676591039845151
3	5.202153905706628	7.104050145124
4	2.3279758800130477	4.2387717826161815
5	1.2349371379004142	2.8107124833096018
6	0.7266242968887612	1.9845531429205407
7	0.44428379139654745	1.4156636353384435
8	0.30236424147497465	1.1010878813994278
9	0.23032462074565144	0.9435924148488946
>10	1.2155464860726022	10.170931252229343
>50	0.12809772020087712	4.111110649107829
>100	0.11635717914854889	10.633591513425229
>500	0.013033243507698999	3.9556967142456894
>1k	0.005495946057463432	4.610369130822481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3840	0.2732205444773163	No Hit
GTCCTACAGTGGACATTTCTAAATT	3551	0.25265785245805994	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3353	0.23856991813344833	No Hit
GATATACACTGTTCTACAAATCCCG	3273	0.23287782345683758	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3260	0.2319528580718883	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3220	0.22910681073358294	No Hit
TTGTAGAACAGTGTATATCAATGAG	2987	0.2125285849879541	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2914	0.2073345485955468	No Hit
GTGTATATCAATGAGTTACAATGAG	2478	0.1763126326080182	No Hit
GTGTATATCAATGAGTTACAATGAA	2346	0.16692067639161043	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2269	0.16144203526537257	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2227	0.1584536855601519	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1763	0.12543953643580952	No Hit
GATATACACTGTTCTACAATGCCGG	1622	0.11540721956828308	No Hit
GTATCAACGCAGAGTACATGGGCAG	1618	0.11512261483445255	No Hit
GTTCTAAGTTGGTTGTTAAGCGTAC	1463	0.10409418139851921	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1445	0.1028134600962818	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1418	0.10089237814292565	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1409	0.10025201749180694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.4230236691526891E-4
7	0.0	0.0	0.0	0.0	1.4230236691526891E-4
8	0.0	0.0	0.0	0.0	1.4230236691526891E-4
9	0.0	0.0	0.0	0.0	1.4230236691526891E-4
10	2.1345355037290334E-4	0.0	0.0	0.0	1.4230236691526891E-4
11	2.1345355037290334E-4	0.0	0.0	0.0	1.4230236691526891E-4
12	2.8460473383053783E-4	0.0	0.0	0.0	2.1345355037290334E-4
13	2.8460473383053783E-4	0.0	0.0	0.0	2.1345355037290334E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGT	35	0.0021677506	16.288311	15
GACCGTT	65	3.3672386E-6	14.617192	7
AAGCGTA	220	0.0	14.253794	18
AATTCGC	40	0.005270697	14.253286	19
CCGCGCG	60	2.5631947E-5	14.252778	9
AGCGTAC	225	0.0	13.936546	19
TAGAAAT	370	0.0	13.604947	4
AGACCGT	70	7.2600833E-6	13.571658	6
AACGTAA	85	2.6943235E-7	13.4134245	10
TTGTAGA	575	0.0	13.210087	1
ACCGTTA	65	5.4417935E-5	13.156411	8
TCCAACG	490	0.0	12.993255	18
GCGTTAT	95	7.4382115E-8	12.992816	1
CGTTATT	110	2.7757778E-9	12.944621	2
GCCCGTA	155	0.0	12.873019	17
TGTAGAA	1095	0.0	12.743655	2
CCCGTAA	150	1.8189894E-12	12.670039	18
GTTAAGC	300	0.0	12.668685	15
TTAAGCG	300	0.0	12.668685	16
ATTCCCG	90	5.397942E-7	12.667782	11
>>END_MODULE