FastQCFastQC Report
Thu 2 Feb 2017
SRR4064124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405458
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG18640.13262580596503062No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16160.11498031246753727No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC15700.11170735802848608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28800.024.6754471
TAGGACC14550.023.7372194
TGTAGGA25650.023.5851442
TACCGTA752.0658863E-623.4650737
CTGTAGG25150.022.9201951
GTAGGAC26150.022.8826263
TACCGTC2900.021.9985057
TAGGACG21900.021.7976064
ACCGTCG2750.021.5993028
GTCCTAC20150.021.4010961
ATACCGT2900.021.2399376
GGACGTG22050.021.0507246
AGGACGT22500.020.8252535
TTAGGAC14200.020.7598923
AGGACCT18250.020.7328385
GACGTGA12150.020.6405747
GTCCTAA11650.020.5852221
TCCTACA21850.020.4379712
GATATAC16050.020.4251961
CGTTCGG550.00448296120.00077612
Produced by FastQC (version 0.11.5)