FastQCFastQC Report
Thu 2 Feb 2017
SRR4064123_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064123_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797032
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT68680.861696895482239No Hit
CTGTAGGACGTGGAATATGGCAAGA56490.708754479117526No Hit
GTCCTACAGTGGACATTTCTAAATT54400.6825321944413775No Hit
CTTTAGGACGTGAAATATGGCGAGG53730.6741260074877796No Hit
GTCCTACAGTGTGCATTTCTCATTT36030.4520521133404932No Hit
ATTTAGAAATGTCCACTGTAGGACG30000.3763964307581126No Hit
CTGAAGGACCTGGAATATGGCGAGA27220.3415170281745275No Hit
CTGTAGGACCTGGAATATGGCGAGA26920.33775306386694637No Hit
GTCCTTCAGTGTGCATTTCTCATTT23630.29647492196047337No Hit
GTATCAACGCAGAGTACTTTTTTTT21470.2693743789458892No Hit
TTTCTAAATTTTCCACCTTTTTCAG19990.25080548836182237No Hit
GATATACACTGTTCTACAAATCCCG17210.21592608577823724No Hit
GAATATGGCAAGAAAACTGAAAATC17140.2150478274398017No Hit
TTGTAGAACAGTGTATATCAATGAG16170.20287767617862268No Hit
TATCAACGCAGAGTACTTTTTTTTT15660.19647893685573475No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15520.1947224201788636No Hit
GGAATATGGCGAGAAAACTGAAAAT14890.18681809513294323No Hit
GTGTATATCAATGAGTTACAATGAA12720.15959208664143976No Hit
ATTCCAGGTCCTTCAGTGTGCATTT12670.1589647592568429No Hit
GCCATATTCCACGTCCTACAGTGGA12650.15871382830300415No Hit
GAAATATGGCGAGGAAAACTGAAAA12380.15532626042618114No Hit
CCATATTCCAGGTCCTTCAGTGTGC11990.15043310682632566No Hit
CTGTAGGACATGGAATATGGCAAGA11830.14842565919561573No Hit
CCATATTTCACGTCCTAAAGTGTGT11300.14177598891888907No Hit
GTGTATATCAATGAGTTACAATGAG11190.140395868672776No Hit
ACCTGGAATATGGCGAGAAAACTGA11040.13851388651898544No Hit
GTCCACTGTAGGACGTGGAATATGG10970.13763562818054983No Hit
ACAGTGGACATTTCTAAATTTTCCA10910.1368828353190336No Hit
GACCTGGAATATGGCGAGAAAACTG10760.13500085316524307No Hit
ATACACACTTTAGGACGTGAAATAT10660.13374619839604934No Hit
TTCCAGGTCCTTCAGTGTGCATTTC10520.13198968171917816No Hit
GTTCTACAGTGTGGTTTTTATCATT10210.12810025193467767No Hit
CCACTGTAGGACGTGGAATATGGCA10090.1265946662116452No Hit
GATATACACTGTTCTACAATGCCGG9960.12496361501169338No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9430.11831394473496672No Hit
GTACATGGGGTGGTATCAACGCAAA9420.11818847925804735No Hit
GTGTATTTCTCATTTTCCGTGATTT9420.11818847925804735No Hit
CACTTTAGGACGTGAAATATGGCGA9340.11718475544269238No Hit
CCTAAAGTGTGTATTTCTCATTTTC9110.11429904947354685No Hit
GAAATACACACTTTAGGACGTGAAA9090.11404811851970813No Hit
CTGTAGAACATATTAGATGAGTGAG8720.1094058958736914No Hit
AGTGTGTATTTCTCATTTTCCGTGA8400.10539100061227154No Hit
CTACAGTGGACATTTCTAAATTTTC8360.10488913870459404No Hit
GTAGGACGTGGAATATGGCAAGAAA8020.10062331248933544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTT250.006026907419.00144412
GTTCCGA307.717528E-419.0002526
AGCTCGT250.00602909319.00025211
TCCGACC350.002166941816.2879738
CGACCAT350.002166941816.28797310
CCGACCA350.002166941816.2879739
TTCCGAC350.002167850716.2869517
AGTTCCG456.75975E-414.7770465
GTTCTAA653.373265E-614.6128251
GCAATTT400.00527345614.25108213
AATCGTA400.00527345614.25108213
CCAACGT759.64741E-713.93439219
ATAATAC551.9643106E-413.8131623
TAGGACC9550.013.7270694
CACCTTT6700.012.76136314
GGCGAGG13850.012.75909219
CCACCTT6800.012.71420213
TTCCACC7100.012.71143611
TTAAGGT604.089846E-412.66603854
TTGTAGA3850.012.3355021
Produced by FastQC (version 0.11.5)